Chemical Characterization and Effects of Oral Administration of Pelargonium graveolens Essential Oil on Testicular Histomorphometry of White Rabbits
Article Sidebar
Main Article Content
Abstract
Pelargonium graveolens is a species of pelargonium known for its aromatic leaves, which are used to produce essential oil. This essential oil is highly valued in aromatherapy for its anti-inflammatory, antibacterial, and skin-healing properties. This study was undertaken to assess the impacts of oral administration of Pelargonium graveolens essential oil (PEO) on fertility enhancement of rabbits' testicles and Gas chromatography coupled with mass spectrometry (GC-MS) examination. The chemical elements of PEO oil were characterized in the physico-chemical center (TPPCA), Algeria. Thirty-one white prepubescent male rabbits belonging to the local population aged 2 months, divided into three groups including one control batch and two experimental batches, administered orally with PEO at 0.4 mL/kg and 0.6 mL/kg /body weight doses. After 21 days of treatment, the rabbits were sacrificed and dissected, the testes were excised, defatted, weighed and preserved in Bouin Holland’s solution. Phytochemical screening of P. graveolens essential oil shows the presence of 26 and 18 bioactive molecules of the plants collected in May and November 2023, respectively. In contrast to the reference group, the macroscopic parameters were higher in the treated groups and the histological analysis demonstrated notable microscopic variations between the treated rabbits and the baseline batch, such as the appearance of the first elongated spermatids in some seminiferous tubules. These results suggest that the P.graveolens essential oil could improve the development and the reproductive process of prepubescent male rabbits.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Noh S, Go A, Kim DB, Park M, Jeon HW, Kim B. Role of Antioxidant Natural Products in Management of Infertility: A Review of Their Medicinal Potential. Antioxidants. 2020; 957 (9):2-61.
Osahon K. Ogbeide 1, Isaac U. Akhigbe, Charles A. Unuigbe, Osayemwenre Erharuyi, Vincent Imieje, Gabriel O. Benjamin, Kingsley Ikeke, Bosede Ayeni, Emmanuel Irabor, Joseph B. Owolabi, Abiodun Falodun. Haematological and Biochemical Examination of Pulcherrimin A isolated from Caesalpinia pulcherrima stem bark. Trp J Nat Prod Res .2021 ;5(11) :2011-2015.
Dayo R. Omotoso, Victoria O. Olubowale, Faith M. Aina, Oore-Oluwapo O. Dramola. Phytochemical Profiling of Basella alba Using Gas Chromatography-Mass Spectrometry.Trop J Nat Prod Res.2024 ;8(6) :7561-7565.
Musino D, Kawahara M, Konoha MK, Yamazaki K. Enhancing Cytotoxicity of Tamoxifen Using Geranium Oil. Evid Based Complementary Altern Med. 2022;2022(1): 1-9.
Al‐Sagheer AA, Mahmoud HK, Reda FM, Mahgoub SA, Ayyat MS. Supplementation of diets for Oreochromis niloticus with essential oil extracts from lemongrass (Cymbopogon citratus) and geranium (Pelargonium graveolens) and effects on growth, intestinal microbiota, antioxidant and immune activities. Aquac Nutr. 2018;24(3):1006-1014.
Laghouati A, Belabbas R, Castellini C, Mattioli S, Dal bosco A, Benkerbane A, Iguer OM. Impact of Algerian date palm pollen aqueous extract on epididymal and ejaculated rabbit sperm motility during in vitro incubation. Ital J Anim Sci. 2021;20(1):717-727.
Sharopov FS, Zhang H, Setzer WN. Composition of geranium (Pelargonium graveolens) essential oil from Tajikistan. Am. J. Essent. Oil. Nat. Prod. 2014; 2 (2): 13-16.
Maher B, Simonds MSJ, Sayadi S, Bouaziz M. Chemical composition and biological activities of polar extracts and essential oil of rose-scented geranium, Pelargonium graveolens. Phytother Res. 2012;27(8):1206-1213.
Džamić AM, Skovie MD, Ristie MS, Grujie SM, Mileski KS, Marin. PD Chemical composition, antifungal and antioxidant activity of Pelargonium graveolens essential oil. J Appl Pharm Sci. 2014;4(03):001-005.
Said AA, Nasr Y, Galal AA, Abdelhamid AE, Mohamed HA, Metwally MM, Said MA, Nassan MA, Dahran N, Mohamed AA. Concerns with Male Infertility Induced by Exposure to Titanium Nanoparticles and the Supporting Impact of Pelargonium graveolens Essential oil: Morphometric Records in Male-Wistar Rats. Life.2022; 639(12):2-19.
Kolai N, Saiah F, Boudia A. In vitro inhibitory effect of Artemisia herba alba essential oil on two strains of Fusarium oxysporum f.sp. Radicis-lycopersici. Alg J Arid Environ. 2012;2(1):71-76.
Ezzat AA, Alkahtani MA, Abdelwareth AAA. Thyme leaves as an eco-friendly feed additive improve both the productive and reproductive performances of rabbits under hot climatic conditions. Vet Med. 2020;65(12):553-563.
Martoja P M., Martoja R. Introduction to animal histology techniques. Ed. Paris: Masson et Cie; 1967.345 p.
Moura A, Souza CE, Erickson BH. Early prepubertal testis criteria, seminiferous epithelium and hormone concentrations as related to testicular development in beef bulls. Sci of Anim Repr. 2011;4(1):39-47.
Boukhatem MN, Hamaidi MS, Saidi F, Hakim Yahia. Extraction, composition, physical and chemical properties of Pelargonium graveolens L. essential oil cultivates in the plain of Mitidja (Algeria). Nat & Technol.2010 ;(3):37-45.
Ben-slima A, Ben-ali M, Barkallah M, Traore AI, Boudawara T, Allouche N, Gdoura R. Antioxidant properties of Pelargonium graveolens L’Her essential oil on the reproductive damage induced by deltamethrin in mice as compared to alpha-tocopherol. Lipids Health Dis. 2013;30(12):2-9.
Boukhris M, Bouaziz M, Feki I, Jemai H, Elfeki A, Sayadi S. Hypoglycemic and antioxidant effects of leaf essential oil of Pelargonium graveolens L’Hér in alloxan-induced diabetic rats. Lipids Health Dis. 2012;81(10):2-10.
Tembhurne SV, Feroz S, More BH, Sakarkar DM. A review on the therapeutic potential of Nigella sativa (kalonji) seeds. J Med Plant Res. 2014; 8(3):167-177.
Soy A, Sahu R, Rath S.A Histomorphological study of the effect of Mint on the testes of albinos Rats. J dental med Sci.2016;15(5):32-35.
Lakabi L and Khelfane- GK. Effects of Mentha piperita, Salvia officinalis, Rosmarinus officinalis essential oils on body weight and testicular weight of prepubescent male rabbits. Ls Res Rl Dev. 2020; 32(8) 123–130.