Antiplatelet and Antithrombotic Properties of 2-Geranyl-2',3,4,4'-tetrahydroxydihydrochalcone, A Chalcone from Artocarpus altilis (Park.) Fosberg Leaves

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Published: Dec 2, 2024
DOI: https://doi.org/10.26538/tjnpr/v8i11.5
Keywords:
Cardiovascular disease, Coronary thrombosis, Platelet aggregation, P2Y12 purinoceptor antagonists, Breadfruit, Dihydrochalcone

Main Article Content

Nanang Fakhrudin
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
Gharsina Ghaisani Yumni
Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
Farah Azura Salsabila
Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
Candra Adianto
Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
Wilda Priskila Iriani Saragi
Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
Yuli Widiyastuti
Center for Research and Development of Medicinal Plants and Traditional Medicine, Tawangmangu, Kalisoro, Karanganyar, Jawa Tengah 57792, Indonesia
Arief Nurrochmad
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
Sitarina Widyarini
Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl Fauna No. 2 Karangmalang, Yogyakarta 55281, Indonesia
Subagus Wahyuono
Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia

Abstract

Thrombosis is a major contributor to the pathophysiology of cardiovascular diseases. The involvement of platelets in the formation of thrombus has been studied extensively. Platelet aggregation inhibition is a promising strategy for the treatment of thrombosis. Artocarpus altilis is a medicinal plant containing 2-geranyl-2',3,4,4'-tetrahydroxydihydrochalcone (GTDC) with antiplatelet activity. A previous study showed that the plant extract and GTDC demonstrated antiplatelet activity in ADP-induced platelet aggregation. However, no data are available regarding its efficacy in platelet aggregation induced by other agonists. In addition, the antithrombotic activity of GTDC is unknown. This study evaluates the antithrombotic activity of GTDC and determines the antiplatelet specificity action of GTDC. Antithrombotic activity assay was done using FeCl3-induced arterial thrombosis in rats, whereas the antiplatelet assays were performed in vitro utilizing human platelet induced by several platelet receptor agonists. One-way ANOVA followed by the Tukey post-hoc test (p = 95%) in GraphPad Prism 8 software were used to analyze the data statistically. We found that GTDC (30 and 60 mg/kg) demonstrated antithrombotic activity by prolonging occlusion time due to thrombosis. GTDC showed its highest selectivity in the platelet aggregation pathway induced by ADP compared with thrombin, arachidonic acid, and epinephrine.

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How to Cite
Fakhrudin, N., Yumni, G. G., Salsabila, F. A., Adianto, C., Saragi, W. P. I., Widiyastuti, Y., Nurrochmad, A., Widyarini, S., & Wahyuono, S. (2024). Antiplatelet and Antithrombotic Properties of 2-Geranyl-2’,3,4,4’-tetrahydroxydihydrochalcone, A Chalcone from Artocarpus altilis (Park.) Fosberg Leaves. Tropical Journal of Natural Product Research (TJNPR), 8(11), 8993 – 8997. https://doi.org/10.26538/tjnpr/v8i11.5
Issue
Vol. 8 No. 11 (2024): Tropical Journal of Natural Product Research (TJNPR)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Nanang Fakhrudin, Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia

Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia

Gharsina Ghaisani Yumni, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia

Faculty of Pharmacy, Universitas Wahid Hasyim, Jl. Raya Manyaran-Gunungpati, Nongkosawit, Kec. Gn. Pati, Kota Semarang, Jawa Tengah 50232, Indonesia

Yuli Widiyastuti, Center for Research and Development of Medicinal Plants and Traditional Medicine, Tawangmangu, Kalisoro, Karanganyar, Jawa Tengah 57792, Indonesia

Research Center for Pharmaceutical Ingredients and Traditional Medicine, The National Research and Innovation Agency (BRIN), Indonesia

Subagus Wahyuono, Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia

Medicinal Plants and Natural Products Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia

Stikes ISFI Banjarmasin, Jl. Flamboyan III no. 7C, Sungai Miai, Banjarmasin, Kalimantan Selatan, 70123, Indonesia

How to Cite

Fakhrudin, N., Yumni, G. G., Salsabila, F. A., Adianto, C., Saragi, W. P. I., Widiyastuti, Y., Nurrochmad, A., Widyarini, S., & Wahyuono, S. (2024). Antiplatelet and Antithrombotic Properties of 2-Geranyl-2’,3,4,4’-tetrahydroxydihydrochalcone, A Chalcone from Artocarpus altilis (Park.) Fosberg Leaves. Tropical Journal of Natural Product Research (TJNPR), 8(11), 8993 – 8997. https://doi.org/10.26538/tjnpr/v8i11.5

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