Effects of Tacrolimus (Prograf) on the Reproductive Efficiency of Mice
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Abstract
Tacrolimus is an immunosuppressant used after organ transplantation to prevent graft rejection. It involves long-term usage which may be associated with detrimental effects. This research aims to investigate the effects of Tacrolimus intake on the reproductive efficiency of mice. Tacrolimus was administered to the test group in doses of 0.5, 1.0, 2.0, and 3.0 mg/kg, while the control groups (positive and negative) were administered 1 ml/kg of distilled water and 20 % methanol, respectively. Reproductive parameters were measured following standard procedure. Male mice in the test group experienced a significant decrease (P<0.05) in their testes, epididymis, and sperm count compared to the control group. The oestrogen levels and gestation index of female mice in the test groups were significantly decreased (P<0.05) in a dose-dependent manner, along with the litter size and weight, in relation to the control group. However, the life birth index of mice did not vary significantly (P>0.05). There was a significant decrease (P<0.05) in the mean values of male and female reproductive parameters. This study has showcases that Tacrolimus capsule intake could cause reproductive organ malfunction and impair potency and fertility.
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