Substrate Effects on the Yield, Proximate, Phytochemical and Vitamin Attributes of Pleurotus pulmonarius Mushroom
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Abstract
The yield attributes and nutrient compositions of Pleurotus pulmonarius under four different substrates (maize-kernel, Empty Palm Fruit Bunch (EPFB), Dry Banana Leaf (DBL), and the Control) were studied in a humid tropical environment. The experimental design was a completely randomized design with nine replications. Variations in the morphological, proximate, vitamin, and phytochemical attributes of the mushrooms were recorded. The data were subjected to statistical analysis. The result revealed a significant substrate effect on the studied attributes. The DBL substrate significantly improved the pileus circumference (39.07 cm), pileus area (124.6 cm), length of stipe (6.37 cm), pileus diameter (12.43 cm), and moisture content in P. pulmonarius, while the maize-kernel substrate had significantly higher protein (4.6%) and flavonoids (14.42%) contents compared to other substrates. The substrate from EPFB gave significantly higher levels of vitamin B1 (3.8 mg/100 g), vitamin B2 (5.7 mg/100 g), vitamin C (114 mg/100 g), and vitamin E (17.4 mg/100 g) than the other substrates. The correlation results reveal that the concentrations of phytochemicals in P. pulmonarius tend to decrease as the morphological attributes expand. This substrate effect can be harnessed to produce mushrooms for specific needs. EPFB substrates can be used to enhance dietary supplementation and antioxidant activity in human nutrition. Mushrooms grown on the substrate formulated with maize kernel could serve as an alternative for animal protein because of their high protein content, while mushrooms produced on the DBL substrate with the highest yield in most attributes are recommended for commercial cultivation of P. pulmonarius.
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References
Adebayo GJ, Omolara BN, Toyin AE. Evaluation of yield of oyster mushroom (Pleurotus pulmonarius) grown on cotton waste and cassava peel. Afr. J. Biotechno. 2009; 8:215-218.
Eze EI, Agbo CU. Nutrient variability and pharmaceutical values of wild mushrooms in a derived savannah zone. In Proceedings of the 38th Annual Conference of Horticultural Society of Nigeria, 2020: p. 46.
Uddin-Pk MM, Sayful-Islam M, Pervin R, Dutta S, Islam-Talukder RJ, Soma N, Rahman M. Enzyme Inhibitory and Antioxidant Activity of Combination of Two Edible Mushrooms of Ganoderma lucidum and Pleurotus ostreatus. Trop J of Nat Prod Res 2018; 2:314–319. doi:10.26538/tjnpr/v2i7.3.
Okhuoya, J.A.O. Edible Mushrooms: As Functional Foods and Nutriceuticals. Trop. J. Nat Prod Res. 2017; 1:186–187. doi:10.26538/tjnpr/v1i5.1.Chang ST, Miles PG. Mushrooms: Cultivation, Nutritional Value, Medicinal Effect and Environmental Impact; CRC Press: Boca Raton, Florida, 2004.
Chang ST, Miles PG. Mushrooms: Cultivation, Nutritional Value, Medicinal Effect and Environmental Impact; CRC Press: Boca Raton, Florida, 2004.
Okhuoya J, Akpaja E, Osemwegie O, Oghenekaro A, Ihayere C. Nigerian Mushrooms: Underutilized non-wood forest resources. J Appl Sci Env Manag 2010; 14:43-54. doi:10.4314/jasem.v14i1.56488.
Baiyeri KP, Chukwudi UP, Chizaram CA, Aneke N. Maximizing rice husk waste for Daucus carota production. Int J Recycl Org Waste Agri. 2019; 8:399-406. doi:10.1007/s40093-019-00312-9.
Ijimbili SB, Adenipekun OC. Comparative study on growth parameters, proximate analysis and mineral composition of Ganoderma lucidum cultivated on different substrates. Adv Food Sci 2022; 44:5-14.
Dávila GL, Murillo AW, Zambrano FC, Suárez MH, Méndez AJ. Evaluation of nutritional values of wild mushrooms and spent substrate of Lentinus crinitus (L.) Fr. Heliyon 2020; 6:1-5. e03502. doi:10.1016/j.heliyon.2020.e03502.
Nnorom IC, Eze SO, Ukaogo PO. Mineral contents of three wild-grown edible mushrooms collected from forests of south eastern Nigeria: An evaluation of bioaccumulation potentials and dietary intake risks. Sci Afri. 2020; 8:1-10. e00163. doi:10.1016/j.sciaf.2019.e00163.
Nwanze PI, Khan AU, Ameh JB, Umoh VJ. The effect of the interaction of various spawn grains with different culture medium on carpophores dry weights and stipe and pileus diameters of Lentinus squarrosulus (mon) singer. Afr. J. Biotechnol 2005; 4:615-619.
Bram VN. Small-scale mushroom cultivation, 2 ed.; Agromisa Foundation and CTA: Wageningen, 2007.
AOAC. Association of Official Analytical Chemists. Official methods of analysis; Washington D. C., 2005.
Madhu M, Sailaja V, Satyadev TNVSS, Satyanarayana M. Quantitative phytochemical analysis of selected medicinal plant species by using various organic solvents. J Pharm Phytochem 2016; 5:25-29.
Obadoni BO, Ochuko PO. Phytochhemical studies and comparative efficacy of the crude extracts of some home static plants in Edo and Delta States of Nigeria. Glo J Pure Appl Sci 2001; 8:203-208.
Onwuka GI. Food Analysis and Instrumentation: Theory and Practice; Napthali Prints: 2005.
Olokodona FA. Analysis of fruit drinks and fruit juices. IPAN Bulletin 2005, 6; 9-14.
Carvalho CSM, Aguiar LVB, Sales-Campos C, Minhoni MTA, Andrade MCN. Applicability of the use of waste from different banana cultivars for the cultivation of the oyster mushroom. Brazilian J Microbiol. 2012; 43:819-826. doi: 10.1590/S1517-83822012000200048
Chukwurah NF, Eze SC, Chiejina NV, Onyeonagu CC, Ugwuoke KI, Ugwu FSO, Onwuelughasi CU. Performance of oyster mushroom (Pleurotus ostreatus) in different local agricultural waste materials. Afri J Biotechnol 2012; 11:8979-8985.
Adebayo EA, Alao MB, Olatunbosun OO, Omoleye EO, Omisakin OB. Yield evaluation of Pleurotus pulmonarius (oyster mushroom) on different agricultural wastes and various grains for spawn production. Ife J Sci 2014; 16:475-480.
Jimoh MA, Akinyemi SA, Olatunji OA, Olowolaju ED, Okunlola GO. Cultivation of Pleurotus ostreatus on agricultural wastes and effects on nutritional composition of the fruiting body. Inter J Veg Sci. 2023; 29:313–321. Doi:10.1080/19315260.2023.2216684.
Garuba T, Abdukkareem KA, Ibrahim IA, Oyebamiji OI, Shoyooye OA, Ajibade TD. Influence of substrates on the nutritional quality of Pleurotus pulmonarius and Pleurotus ostreatus. Ceylon J Sci 2017; 46: 7-74 Doi: 10.4038/cjs.v46i1.7419
Elattar AM, Hassan S, Awd-Allah SF. Evaluation of oyster mushroom (Pleurotus ostreatus) cultivation using different organic substrates. Alexandria Sci Exch J 2019; 40:427-440.
Dunkwal V, Jood S. Effect of substrates on nutrient composition of oyster mushroom (Pleurotus sajor caju). J Dairying, Foods Home Sci. 2009; 28:132-136.
Rózsa S, Măniuțiu DN, Poșta G, Gocan TM, Andreica I, Bogdan I, Rózsa M, Lazăr V. Influence of the culture substrate on the Agaricus blazei Murrill mushrooms vitamins content. Plants 2019; 8:1-19. 316. Doi: 10.3390/plants8090316
Egwim EC, Elem RC, Egwuche RU. Proximate composition, phytochemical screening and antioxidant activity of ten selected wild edible Nigeria mushrooms. American J Food Nutr 2011; 1:89-94.
Tsao R. Chemistry and biochemistry of dietary polyphenol. J of Nutr 2010; 2:1231-1246.
Chukwudi UP, Agbo CU, Echezona BC, Eze EI, Kutu FR, Mavengahama S. Variability in morphological, yield and nutritional attributes of ginger (Zingiber officinale) germplasm in Nigeria. Res Crops. 2020; 21:634-642. Doi:10.31830/2348-7542.2020.099.