Effects of Different Cultural Conditions on Phosphate Solubilisation in Pseudomonas mosselii D7 and Aspergillus niger Vitaf1
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Abstract
Microbial phosphate (P) solubilisation has been reportedly impacted by various environmental conditions. Therefore, this study was aimed at investigating the effects of different cultural/environmental conditions on phosphate solubilisation in bacteria and fungi. Phosphate-solubilising microorganisms (PSM) were isolated from rhizospheric soil of tomatoes, cowpea, and corn and then screened for phosphate solubilsation on Pikovskaya (PVK) agar. Effects of different environmental conditions on phosphate solubilisation were evaluated by cultivating PSM in National Botanical Research Institute's Phosphate growth medium (NBRIP) amended with different carbon and nitrogen sources, salt concentrations and maintained at varying pH and temperature regimes. Five strains of the microbes produced halo zones on PVK agar. Isolate NF1 had the highest solubilisation index (4.0), followed by NB3 (3.5) and NF2 (3.45), NB1 (2.0), and then NB2 (1.0). In terms of supporting P solubilisation, carbon sources performed in the order: glucose>fructose>sucrose> maltose>mannitol in the best performed fungal (NF1) and bacterial (NB3) isolates (P≤0.05). Similarly, the nitrogen sources performed in the following order: (NH4)2SO4>NaNO3>Ca(NO3)2>KNO3 (P≤0.05)in both NF1and NB3. pH 7.0, 30 oC and 0% NaCl were found to support the highest quantity of P solubilised. The highest P solubilised was 0.67 mg/mL in NB3 and 0.72 mg/mL in NF1. Given the conditions employed in this study, the fungal isolate was more efficient in phosphate solubilisation than its bacterial counterpart. NF1 and NB3 were identified through microbiological and molecular methods as Pseudomonas mosseliid7 and Aspergillus niger VITAF1 respectively. Therefore, optimum solubilisation was dependent on the interplay between organisms and their environmental conditions.
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Guan F, Xia M, Tang X, Fan S. Spatial variability of soil nitrogen, phosphorus and potassium contents in moso bamboo forests in Yong'an City, China. Catena. 2017; 150: 161-172.
Zhang Y, Chen FS, Wu XQ, Luan FG, Zhang LP, Fang XM, Wan SZ, Hu, SF, Ye JR. Isolation and characterization of two phosphate-solubilising fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments. PLoS ONE. 2018; 13(7): e0199625.
Aliyat FZ, Maldani M, El Guilli M, Nassiri L, Ibijbijen J. Phosphate-Solubilising Bacteria Isolated from Phosphate Solid Sludge and Their Ability to Solubilise Three Inorganic Phosphate Forms: Calcium, Iron, and Aluminum Phosphates. Microorganisms. 2022; 10(5):980
Bouwman AF, Beusen AHW, Lassaletta L, Apeldoorn DFV, Grinsven HJMV, Zhang J. Lessons from temporal and spatial patterns in global use of N and P fertilizer on cropland. Sci Rep. 2017; 7:40366.
Heppell J, Payvandi S, Talboys P, Zygalakis KC, Fliege J, Langton D Sylverster-Bradley R, Walker R, Jones D, Roose T.Modelling the optimal phosphate fertiliser and soil management strategy for crops. Plant Soil. 2016; 401(1-2):135-149.
Bargaz A, Elhaissoufi W, Khourchi S, Benmrid B, Borden KA, Rchiad Z. Benefits of phosphate solubilising bacteria on belowground crop performance for improved crop acquisition of phosphorus. Microbiolo Res.. 2021; 252:126842.
Sarr PS, Tibiri EB, Fukuda M, Zongo AN, Compaore E, Nakamura S. Phosphate-solubilising fungi and alkaline phosphatase trigger the P solubilisation during the co-composting of sorghum straw residues with Burkina Faso phosphate rock. Front. Environ. Sci. 2020; 8:559195.
Tang X, Xia M, Guan F, Fan S. Spatial distribution of soil nitrogen, phosphorus and potassium stocks in moso bamboo forests in subtropical China. Forests. 2016; 7(11): 267.
Edi-Premono M, Moawad MA, Vleck PLG. Effect of phosphate solubilizing Pseudmonas putida on the growth of maize and its survival in therhizosphere. Indonesian J. Crop Sci 1996, 11: 13-23
Nautiyal, C.S. An efficient microbiological growth medium for screening phosphate solubilising microorganisms, FEMS Microbiol. Lett 1999,170(1) 265–270.
Danaski AI, Shugaba A, Milala MA, Ndirmbula JB, Gidado A. Isolation, identification and pathogenicity study of the microbes causing tomato post-harvest spoilage in Maiduguri metropolis, Maiduguri, Nigeria. NJBMB, 2022; 37(4): 303-313.
Mardad I, Serrano A, Soukri A. Solubilization of inorganic phosphate and production of organic acids by bacteria isolated from a Moroccan mineral phosphate deposit. Afr. J. Microbiol. Res. 2013; 7:626-635.
Bolo P, Kihara J, Mucheru-Muna M, Njeru EM, Kinyua M, Sommer R. Application of residue, inorganic fertilizer and lime affect phosphorus solubilising microorganisms and microbial biomass under different tillage and cropping systems in a Ferralsol. Geoderma. 2021, 390:114962.
Dhanya RT, Deepthi H, Pravitha MS, Lecturer D. Isolation of phosphate solubilising bacteria and fungi from rhizospheres soil from banana plants and its effect on the growth of Amaranthus cruentus L. J Pharm Biol Sci. 2013; 5:6-11.
Suleman M, Yasmin S, Rasul M, Yahya M, Atta BM, Mirza MS. Phosphate solubilising bacteria with glucose dehydrogenase gene for phosphorus uptake and beneficial effects on wheat. PLoS One. 2018;13(9): e0204408
Hii YS, San CY, Lau SW, Danquah MK. Isolation and characterisation of phosphate solubilising microorganisms from peat. BiocatalAgricBiotechnol. 2020; 1(26):101643.
Oyeagu U, Ughamba KT, Ekwem OH, Ugwu FSO, Ezeh CC. Penicillium citrinum: A Promising Candidate for Protease Production. Trop J Nat Prod Res. 2023; 7(9):4092-4098
Mardad I, Serrano A, Soukri A . Effect of Carbon, Nitrogen Sources and Abiotic Stress on phosphate solubilisation by bacterial Strains Isolated from a Moroccan rock phosphate Deposit. J Adv. Chem. Eng. (2014); 4(1): 1-10.
Vassileva M, Vassilev N, Azcon R. Rock phosphate solubilisation by Aspergillus niger on olive cake based medium and its further application in a soil plant system. World J Microbiol Biotechnol. 1998; 14:281-284.
Sanchez-Gonzalez ME, Mora-Herrera ME, Wong-Villarreal A, De La Portilla-López N, Sanchez-Paz L, Lugo J, Vaca-Paulín R, Del Aguila P, Yañez-Ocampo G. Effect of pH and carbon source on phosphate solubilisation by bacterial strains in pikovskaya medium. Microorganisms. 2022, 11(1):49.
Relwani L, Krishna P, Sudhakara RM. Effect of carbon and nitrogen sources on phosphate solubilsation by wild-types strain and UV-induced mutants of Aspergillus tubingensis. CurrMicrobiol. 2008; 5(5): 401-406.
Scervino J, Papinutti MVL, Godoy MS, Rodriguez, MA, Della Monica I, Recchi M, Pettinari MJ, Godeas AM. Medium pH, carbon and nitrogen concentrations modulate the phosphate solubilization efficiency of Penicillium purpurogenum through organic acid production. J Appl Microbiol. 2011; 110:1215-1223.
Pallavi KP. Effect of nitrogen sources on phosphate solubilising effieciencyof psychrotolerant bacterial strains. The Pharm Innov J 2023; 12(8): 486-489
Sagervanshi A, Kumari P, And AN, Kumar A. Isolation and characterization of phosphate solubilising bacteria from Anand agriculture soil. Int J Phama Life Sci. 2012; 2:256-266.
Abdelhady HM, Abou-Taleb KA, Abd el-salam SS, Amin SA, Tayeb ZH. effect of carbon and nitrogen sources on phosphate solubilisation by some local isolates from Egyptian rock phosphate deposit.Res J Pharm BiolChem Sci. 2017; 8(6):452-469.
Pallavi KP, Gupta PC. Effect of different carbon and nitrogen sources on solubilisation of insoluble inorganic phosphate by psychrotolerant bacterial strains. The Bioscan. 2013; 8(4):1299-1302.
Vikram S, Himanshu P, Shreyas B. Optimization of various culture conditions for phosphate solubilisation by Burkholderia latens isolated from Bt-cotton rhizospheric soil. Res. J. Biotech. 2022,17 (10):98-105
Sanchez-Gonzalez ME, Mora-Herrera ME, Wong-Villarreal A, De La Portilla-López N, Sanchez-Paz L, Lugo J, Vaca-Paulín R., Del Aguila P, Yañez-Ocampo, G. Effect of pH and Carbon Source on Phosphate Solubilisation by Bacterial Strains in Pikovskaya Medium. Microorg2023, 11, 49:1-11
Wang Y, Li S, Zhang, B, Wang, Y, Meng J, Gao, Y, He X, and Hu X.. Identification of phosphate solubilising microorganisms and determination of their phosphate solubilising activity and growth promoting capacity. Bioresour. 2020, 15(2): 2560-2578.
Walpola B.C., Song J.S., Keum M.J. and Yoon M.H., Evaluation of phosphate solubilising potential of three Burkholderia species isolated from green house soils. Korean J. Soil Sci. Fert. 2012, 45(4), 602-609
Srividya S, Soumya S, Pooja K.Influence of environmental factors and salinity on phosphate solubilisation by a newly isolated Aspergillus niger F7 from agricultural soil. Afr. J. Biotechnol. 2009, 8 (9): 1864-1870
Walpola BC, Arunakumara K, Yoon M. Isolation and characterization of phosphate-solubilising bacteria (Klebsiella oxytoca) with enhanced tolerance to environmental stress. Afr J. Microbiol. Res. 2014, 8(31):2970-2978
Maheswar NU, Sathiyavani G. Solubilisation of phosphate by Bacillus Spp, from groundnut rhizosphere (Arachis hypogaea L). J Chem Pharm Res. 2012; 4:4007–4011.
Patil V. Bacillus subtilis: A potential salt tolerant phosphate solubilising bacterial agent. Int J Life Sci Biotechnol Pharma Res. 2014; 3:141.