The Effect of Gambir Catechins (Uncaria gambir (Hunter) Roxb.) on Improving the Memory of Male Mice Using the Morris Water Maze Test
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Abstract
Memory is a very important component of the cognitive function of every individual. One of the causes of memory decline is the onset of oxidative stress. The Gambir plant (Uncaria gambir (Hunter) Roxb) contains catechin compounds that have been proven to be able to repair oxidative damage, so it is thought to improve memory. The purpose of this study was to determine the effect of Gambir catechins on improving the memory of male white mice using the Morris Water Maze test with the parameter of latency time (the time it takes to reach the platform). This study used 25 male white mice (Mus musculus) divided into 5 control groups of 5 animals each, namely the negative control group (Na CMC 0.5%) and the positive control group, as well as 3 treatment groups with pure catechin compounds (0.875; 1.75; 3.5 mg/kgBW). Test animals were given 10% ethanol orally as a memory-lowering inductor. Each group was trained on the Morris Water Maze (MWM) test equipment in 2 stages, namely the acquisition trial and probe trial stage. The results obtained which was analyzed using a one-way ANOVA statistical test and then with the Duncan test. The statistical results showed that there was a significant difference between the treatment group and the negative control group with a significant value of p<0.05. The results showed that each treatment with a dose of 0.875; 1,75; and 3.5 mg/kgBW of Gambir catechins, provided functional improvements in the memory of male mice.
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