GC-MS Analysis, Antidiarrhoeal, and In Vitro Antioxidant Activities of Ethanol Extract of Blumea balsamifera (L.) DC. Leaves from North Sumatra Province
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Abstract
Blumea balsamifera leaves exhibit biological activities as antibacterial, antidiarrhoeal, and antioxidant agents. This study aims to identify chemical compounds with potential antibacterial, antidiarrheal, and antioxidant activities through in vitro methods. The study used GC-MS to determine the chemical components in Blumea balsamifera leaves and evaluated antidiarrhoeal activity through an antibacterial approach using the Kirby Bauer method against Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Helicobacter pylori, and Escherichia coli with extract concentrations of 0% (DMSO), 10%, 30%, 50%, 70%, and 100%. Antioxidant activity was assessed using the DPPH method. The results of the GC-MS analysis showed that the EtOH extract of Blumea balsamifera leaves contains isopropanol, azelaic acid (AZA), palmitic acid (PA), myristyl alcohol, (Z)-hexadec-11-en-1-ol, and hendecanoic acid. The 10-100% ethanol extract demonstrated antibacterial activity against diarrhoea-causing bacteria, while the antioxidant activity of the EtOH extract showed an IC50 value of 117.560 ppm, and ascorbic acid had an IC50 value of 3.358 ppm. The EtOH extract of Blumea balsamifera leaves demonstrated potential as an antibacterial, antidiarrhoeal, and antioxidant agent in vitro.
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