GC-MS Analysis, Antidiarrhoeal, and In Vitro Antioxidant Activities of Ethanol Extract of Blumea balsamifera (L.) DC. Leaves from North Sumatra Province
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Abstract
Blumea balsamifera leaves exhibit biological activities as antibacterial, antidiarrhoeal, and antioxidant agents. This study aims to identify chemical compounds with potential antibacterial, antidiarrheal, and antioxidant activities through in vitro methods. The study used GC-MS to determine the chemical components in Blumea balsamifera leaves and evaluated antidiarrhoeal activity through an antibacterial approach using the Kirby Bauer method against Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Helicobacter pylori, and Escherichia coli with extract concentrations of 0% (DMSO), 10%, 30%, 50%, 70%, and 100%. Antioxidant activity was assessed using the DPPH method. The results of the GC-MS analysis showed that the EtOH extract of Blumea balsamifera leaves contains isopropanol, azelaic acid (AZA), palmitic acid (PA), myristyl alcohol, (Z)-hexadec-11-en-1-ol, and hendecanoic acid. The 10-100% ethanol extract demonstrated antibacterial activity against diarrhoea-causing bacteria, while the antioxidant activity of the EtOH extract showed an IC50 value of 117.560 ppm, and ascorbic acid had an IC50 value of 3.358 ppm. The EtOH extract of Blumea balsamifera leaves demonstrated potential as an antibacterial, antidiarrhoeal, and antioxidant agent in vitro.
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Yi R, Tian Y, Tan F, Li W, Mu J, Long X, Yanni P. and Zhao X. Intervention effect of Malus pumila leaf flavonoids on senna‐induced acute diarrhea in BALB/c mice. J Food Sci Nutr.2020;8(5):2535-2542.
Dawurung CJ, Usman JG, Gotep JG, and Pyne SG. The Roots of Neorautanenia mitis (A. Rich) Verdcourt: Further Evidence of Its Antidiarrhoeal Activity. Molecules.2023;28(2):1–9.
Wibowo DA, Nailufar F, and Tjandrawinata RR. Antidiarrheal effect of dlbs1y62, a bioactive fraction of Uncaria gambir roxb. Dried sap extract, in wistar rats. J Pharmacol Exp Ther.2021;13:669–675.
Argudín MÁ, Mendoza MC, and Rodicio MR. Food Poisoning and Staphylococcus aureus Enterotoxins. Toxins (Basel).2010;2(7):1751–1774.
Safavi M, Shams-Ardakani M, and Foroumadi A. Medicinal plants in the treatment of Helicobacter pylori infections. Pharm Biol.2015;53(7):939–960.
Kilmer PD. Review Article: Review Article. Journalism. 2010;11(3):369–373.
Kakiuchi T, Nakayama A, Shimoda R, and Matsuo M. Atrophic gastritis and chronic diarrhea due to Helicobacter pylori infection in early infancy: A case report. Med (United States). 2019;98(47).
Batubara Y and Suwardi, A. B. Ethnobotanical Study of Medicinal Plants by the Batak Tribe in Manisak Village, North Sumatra. J. Ethnobot. Study Tradit. Med. Dev. Med..2024;1(2):72-84.
Pang Y, Wang D, Fan Z, Chen X, Yu F, Hu X, Wang K and Yuan L. Blumea balsamifera- A phytochemical and pharmacological review. Molecules.2014;19(7):9453–9477.
Wannes WA, and Tounsi MS. Phytochemical composition and health properties of Lycium europaeum L.: A review. Acta Ecol Sin. 2021;41(5):390–401.
Kong J, Huang X, Yang X, Zhang L, He X, Liu Q, Wu H and Wang X. Network pharmacology-based prediction of potential targets of ethnic medicine Blumea balsamifera (L.) DC acting on anti-infammatory effect. J Phys Conf. Ser.2020;1486(2).
Pang YX, Fan ZW, Wang D, Yang Q, Wang K, Chen XL, Xuan H, Fu LY, and Zhen XC. External application of the volatile oil from Blumea balsamifera may be safe for liver - A study on its chemical composition and hepatotoxicity. Molecules.2014;19(11):18479–18492.
Wang J, He H, Zhou Z, Bai L, She X, He L, He Y, and Tan D. Chemical constituents and bioactivities of Blumea balsamifera (Sembung): a systematic review. Food Sci Technol. 2023,43:1-12.
Sari NM, Aryani F, Wartomo W, Hernandi MF, Rositah E, and Prayitno J. Phytochemical and Antioxidant Activity of Blumea balsamifera and Cordyline fruticosa Based on Ethnopharmacology Knowledge of Muara Tae Tribe, East Kalimantan. Biol Med Nat Prod Chem.2023;12(1):273–280.
Ismail N, A Matawali, A Kanak, F A, Lee PC, H SE, Goh L P W, and Gansau J A. Antimicrobial activities and phytochemical properties of Blumea balsamifera against pathogenic microorganisms. J. Med. Lab. .2022;15(8):951-954.
Jirakitticharoen S, Wisuitiprot W, Jitareerat P, and Wongs-Aree C. Phenolics, antioxidant and antibacterial activities of immature and mature Blumea balsamifera leaf extracts eluted with different solvents. J Trop Med.2022(1):1-12.
Masyudi, Anita N, Muhammad R, Salfauqi N, Jailani, Armi, TMR, Said U, Muhammad H and Marlina. Antibacterial activity of Blumea balsamifera leaf extracts from Aceh, Indonesia. Biodiversitas.2023;24(8):4584-4589.
Dai L, Cai S, Chu D, Pang R, Deng J, Zheng X, and Dai W. Identification of chemical constituents in Blumea balsamifera using UPLC–Q–Orbitrap HRMS and evaluation of their antioxidant activities. Molecules.2023;28(11):1-16.
Ijoma KI, Ajiwe VIE and Ndubuisi JO. Evidence-Based Preferential in vitro Antisickling Mechanism of There Native Nigerian Plants Used in The Management of Sickle Cell Disease. MJBMB. 2022(3):9-17.
Ginting B, Maulana I, Yahya M, Saidi N, Murniana M, Hasballah K, Maulidna M, and Rawati, S. Antioxidant and antiproliferative activities of n-hexane extract and its fractions from Blumea balsamifera L. leaves. JAPTR.2022;13(3):216-220.
Tarfaoui K, Brhadda N, Ziri R, Oubihi A, Imtara H, Haida SH, Ouhssine M, Asma S, Mohammad KP, Saad F and Mohammed O. Chemical profile, antibacterial and antioxidant potential of Zingiber officinale Roscoe and Elettaria cardamomum (L.) maton essential oils and extracts. Plants.2022;11(11):1-14.
Falodun A, Siraj R, and Choudhary MI. GC-MS analysis of insecticidal leaf essential oil of Pyrenacantha staudtii Hutch and Dalz (Icacinaceae). Trop J Pharm Res.2009;8(2):139-143.
Islam ME, Islam KMD, Billah MM, Biswas R, Sohrab MH, and Rahman SM. Antioxidant and anti-inflammatory activity of Heritiera fomes (Buch.-Ham), a mangrove plant of the Sundarbans. ADTM.2020; 20:189-197.
Aboualizadeh E, Violet VB, Daniella SMM, Janis T, Carol J, Chukuka S and Enwemwka. Understanding the antimicrobial activity of selected disinfectants against methicillin-resistant Staphylococcus aureus (MRSA). Plos One.2017:1-15.
Dinler BS, and Cetinkaya H. An overview on Azelaic Acid: Biosynthesis, signalling and the action under stress conditions in plants. J. Plant Sci. Phytochem .2024;10:8-12.
Ghasemi S, Sabour Z, Moazen M, and Manoochehri S. Preparation. Investigation of Physicochemical Properties and Stability of Azelaic Acid Gel Formulation. J Guilan Univ Med Sci.2024;33(1),74-83.
Spaggiari C, Giannamaria A, Costanza S, Sabrina LM, Mattia I, Clotilde SC and Gabrielle C. Extraction and Quantification of Azelaic Acid from Different Wheat Samples (Triticum durum Desf.) and Evaluation of Their Antimicrobial and Antioxidant Activities. Molecules. 2023;28:1-13.
Wang X, Chaonan Z and Na B. Molecular mechanism of palmitic acid and its derivates in tumor progression. Front oncol.2023;13:1-10.
Dash S, Sahoo N, Pattnaik G, Das C, Pattanaik S, Bhar K, and Kar B. GC-MS Profiling, Antimicrobial Activity of Annona squamosa: An In-silico and Inv-itro Approach.Trop J Nat Prod Res.2023;7(8): 3691-3700.
Davoodbasha M, Edachery B, Nooruddin T, Lee S. Y, and Kim JW. An evidence of C16 fatty acid methyl esters extracted from microalga for effective antimicrobial and antioxidant property. Microb Pathog.2018;(115):233-238.
Cetin B, Ahmet C, Hakan O, Ozkan A and Ebru M. Antimicrobial Activities of Essential Oils and Hexane Extracts of Two Turkish Spice Plants, Cymbocarpum erythraeum (DC.) Boiss. and Echinophora tenuifolia L. Against Foodborne Microorganisms. Rec Nat Prod.2016;10(4):426-436.
Kim TJ, Sinn DH, Min YW, Son HJ, Kim JJ, Chang Y, Sun YB, Soo HA, Hyuk L and Ryu S. A cohort study on Helicobacter pylori infection associated with nonalcoholic fatty liver disease. J Gastroenterol.2017;(52):1201-1210.
Mustapha MB, Afifa ZB, Ikbel C, Guido F, Roberta A and Hichem BJ. Chemical Composition and Insecticidal Activity of Crithmum Maritimum L. Essential Oil against Stored-Product Beetle Tribolium Castaneum. Chem Biodiversity.2020;17(4):1-13.
Masyudi, Hanafiah M, Rinidar, Usman S, Marlina. Phytochemical screening and GC-MS analysis of bioactive compounds of Blumea balsamifera leaf extracts from South Aceh, Indonesia. Biodiversitas.2022;23(3):1346–1352.
Godlewska ZB, Świsłocka R, Kalinowska M, Golonko A, Świderski G, Arciszewska Ż, Sieliwoniuk ED, Naumowicz M and Lewandowski W. Biologically active compounds of plants: Structure-related antioxidant, microbiological and cytotoxic activity of selected carboxylic acids. Materials.2020;13(19):1-37.
Mykhailenko O, Gudžinskas Z, Romanova S, Orlova T, Kozyra S, Harna S, and Volochai, V. The comparative analysis of carboxylic acid composition of four Iris species from Ukraine. Chem Biodiversity.2020;18(3):1-9.
Marchyshyn S, Slobodianiuk L, Budniak L, and Skrynchuk, O. Analysis of carboxylic acids of Crambe cordifolia Steven. PharmD.2020;68(1):15-21.
Bredael K, Geurs S, Clarisse D, De Bosscher K, and D’hooghe M. Carboxylic acid bioisosteres in medicinal chemistry: synthesis and properties. J Chem.2022(1):1-21.
Heryanto R, Anindya W, Heliawati L, Septaningsih DA, Karomah AH, Rahminiwati M, Dyah I and Rafi M. Phytochemical Profiling by Lc-Hrms and Antioxidant Activity of Blumea balsamifera Leaves Extract Based on Differences in The Growing Location and Extracting Solvent. Farmacia.2024;72(3):613-621.
Sukma W, Boonyakong C, Chaiphet T, and Chamni S. A simple and rapid colorimetric detection of methanol, ethanol, and isopropanol and its application toward alcohol-based hand sanitizer quality control. Green Chem. Lett. Rev. 2024;17(1):1-12.
Cikoš AM, Flanjak I, Bojanić K, Babić S, Čižmek L, Čož-Rakovac R, Stela J and Jerković I. Bioprospecting of coralline red alga Amphiroa rigida JV Lamouroux: Volatiles, fatty acids and pigments. Molecules.2021;26(3):1-21.
Bergsson G, Hilmarsson H, and Thormar H. Antibacterial, antiviral and antifungal activities of lipids. Lipids and essential oils as antimicrobial agents. Wiley Online Library.2011; 47-80p.
Xiong Y, Yi P, Li Y, Gao R, Chen J, Hu Z, Lou H, Du C, Zhang J, Zhang Y, Yuan C, Huang L, Hao X and Gu W. New sesquiterpeniod esters form Blumea balsamifera (L.) DC. and their anti-influenza virus activity. Nat Prod Res [Internet].2022;36(5):1-10.
Jabri MA, Rtibi K, Sakly M, Marzouki L, and Sebai H. Role of gastrointestinal motility inhibition and antioxidant properties of myrtle berries (Myrtus communis L.) juice in diarrhea treatment. Biomed Pharmacother.2016;84:1937-1944.
Radulovic NS, Blagojevic PD, Stojanovic-Radic ZZ, and Stojanovic NM. Antimicrobial plant metabolites: structural diversity and mechanism of action. Curr Med Chem. 2023;20(7):932-952.
Song L, Hu X, Ren X, Liu J, and Liu, X. Antibacterial modes of herbal flavonoids combat resistant bacteria. Front Pharmacol.2022;13:1-11.
Rasonabe ZMP, Cruz JD, Tiausas CG, Areza F. Chemical composition and antimicrobial activity of the extracts and essential oil of Blumea balsamifera from the Philippines. Int J Herb Med.2023;11(1):06–14.
Simanjuntak HA, and Rahmiati R. Antibacterial and antifungal activities of patikan kebo (Euphorbia hirta L.) Herb Ethanol Extract. Asian J. Pharm. Res. Dev. 2021;9(5), 6-9.
Kusumawati IGAW, Atikawati, Putra IMW, Yogeswara IBA. Antioxidant activity and α-amylase inhibitory of herbal drink from the combination of Blumea balsamifera L and Coccinia grandis L. Food Res.2022;6(4):329–336.
Kusumawati IGAW, Reyunika IN, Yogeswara IBA, Mustika IG, Adi Putra IMW, Santoso U, and Yustinus M. Effect of loloh sembung (Blumea balsamifera) maturity stage on antioxidant activity. Indonesian J Nutr Diet.2019;6(1):1-6.
Okolie N P, Falodun A, and Davids O. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetia tripetala) and it’s potential for the inhibition of lipid peroxidation. Afr. J. Tradit. Complement. Altern. Med. 2014;11(3):221-227.
Yuan Y, Huang M, Pang YX, Yu FL, Chen C, Liu LW, Zhen XC, Ying BZ, Xiao LC and Hu X. Variations in essential oil yield, composition, and antioxidant activity of different plant organs from Blumea balsamifera (L.) DC. at different growth times. Molecules.2016;21(8):1-13.
Njus D, Kelley PM, Tu YJ, and Schlegel HB. Ascorbic acid: The chemistry underlying its antioxidant properties. Free Radic Biol Med.2020;159:37