Ameliorative Effect of Ethanol Extract Gel of Kirinyuh (Chromolaena odorata L.) on Aphthous Stomatitis in Wistar Rats (Rattus novergicus)
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Abstract
Aphthous stomatitis is a self-limiting condition, but its presence interferes with chewing, swallowing, and speaking. Kirinyuh (Chromolaena odorata L.) leaves are natural ingredient that is used traditionally as a wound healing medicine. This study aimed to evaluate the ameliorative effect of ethanol extract gel of kirinyuh on aphthous stomatitis in rats. Twenty-five (25) Wistar rats were divided into five groups of 5 animals each: Group I (HPMC), Group II (Hyaluronic Acid), Groups III - V (2%, 4%, and 8% C. odorata ethanol extract gel, respectively). A wound was made on the ventral tongue of the rats with a heated burnisher. Following wound induction, the rats were administered HPMC, hyaluronic acid, 2%, 4%, and 8% C. odorata leaves ethanol extract gel according to the grouping. The body weights of the rats were monitored, the wound healing, and inflammation were assessed on days 3, 5, and 7. Histopathological examination of the tongue tissues was also carried out. The results showed that the treatments had no significant influence on the body weight of the rats. C. odorata ethanol extract gel resulted in a concentration-dependent increase in the percentage wound healing and inflammation recovery, with the 8% C. odorata gel exhibiting the highest activity (100% on day 7). Histopathological examination showed that the mean epidermal thickness, the number of fibroblasts and neurovasculature were highest in the 8% C. odorata ethanol extract gel treated group. Therefore, C. odorata ethanol extract gel is effective in the treatment of aphthous stomatitis wounds.
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