LC-MS Analysis, Total Phenolics Content, and DPPH Radical Scavenging Activity of Juvenile Juglans regia L. Fruit (walnut) Acetonitrile Extract
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Abstract
Juglans regia L. is a fragrant transitory tree. Numerous health advantages are linked to the different components of the tree's phytochemistry, which has been the subject of substantial research. The current study evaluated the chemical composition of Jordanian Juglans regia Linn. acetonitrile extracts. The extract from the Juglans regia L. juvenile fruit, which grows in Amman, Jordan, was examined to determine its photochemical components. The extract was subjected to high-performance liquid chromatography-tandem mass spectrometry analysis. The fruit was harvested in May 2023. The juvenile fruit was extracted using Soxhlet, yielding 5.5 per cent. The
semi-quantitative analysis identified secondary metabolites, including tannins. The total phenol content reached 9.83 gallic acid equivalents. Spectrophotometric analysis assessed antiradical activity after 2,2-diphenyl-1-picrylhydrazyl reduction, with an IC50 value of 0.75 mg/mL. The study revealed 79 chemical compositions, including gingerol, hyperoside flavonoids, prostaglandin derivatives, quercetin, naringenin, and ginkgolic acid II. These compounds have previously shown antibacterial, anti-inflammatory, and ulcer-prevention properties. Overall, this work shows that several phenolic acids, flavonoids, and fatty acids were identified that correspond
to the pharmacological action of Juglans regia L. immature fruit extract previously described. The structural elucidation of unknown peaks requires more research.
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