Phytoconstituents Screening and Analysis of Antioxidant, Membrane Stabilizing, and Thrombolytic Properties of Leaves and Rhizomes of Schumannianthus dichotomus (Roxb.) Gagnep

Main Article Content

Syeda F. T. Zahra
Mohammad Shahriar
Fatema T. Zohora
Sajan Das
Rumana Akter
Mohiuddin A. Bhuiyan

Abstract

The initial screening of various leaf and rhizome extracts of Schumannianthus dichotomus (Roxb) indicated the presence of saponins, phenols, flavonoids, glycosides, phytosterols, and tannins. To verify the therapeutic potential of this plant, known locally as Shital Pati, the antioxidant activity of the different extracts from its leaves and rhizomes were evaluated using eight methodologies. Using a Soxhlet extraction device, solvents like methanol, ethanol, and chloroform were used to extract the powdered leaves and rhizomes of this plant. The membrane stabilizing and thrombolytic potential of the extracts were also assessed using the clot lysis method. In the DPPH
scavenging test, the ethanol extracts of the leaf and rhizome showed the highest levels of free radical scavenging activity (IC50 22.54 and 19.78 μg/mL, respectively), compared to the standard (IC50 19.51 μg/mL). In the H2O2 scavenging assay, the chloroform leaf extract and methanol rhizome extract exhibited the highest inhibition with IC50 values of 2.03 and 1.85 µg/mL, respectively, compared to the standard L-Ascorbic acid at 1.69 µg/mL. The leaf and rhizome extracts displayed promising potential in total antioxidant, total phenolic content, and cupricreducing antioxidant capacity. The results indicated that all leaf and rhizome extracts have significant potential for membrane stabilizing activity compared to the standard. The methanol leaf and ethanol rhizome extracts of S. dichotomus have demonstrated better thrombolytic activity with 23.74±10.71 and 23.56±1.65% clot lysis, respectively, compared to the standard
streptokinase at 24.14±9.5%. This investigation confirms the therapeutic potential of S. dichotomus's leaves and rhizome.

Downloads

Download data is not yet available.

Article Details

How to Cite
Zahra, S. F. T., Shahriar, M., Zohora, F. T., Das, S., Akter, R., & Bhuiyan, M. A. (2024). Phytoconstituents Screening and Analysis of Antioxidant, Membrane Stabilizing, and Thrombolytic Properties of Leaves and Rhizomes of Schumannianthus dichotomus (Roxb.) Gagnep. Tropical Journal of Natural Product Research (TJNPR), 8(9), 8474-8481. https://doi.org/10.26538/tjnpr/v8i9.33
Section
Articles

How to Cite

Zahra, S. F. T., Shahriar, M., Zohora, F. T., Das, S., Akter, R., & Bhuiyan, M. A. (2024). Phytoconstituents Screening and Analysis of Antioxidant, Membrane Stabilizing, and Thrombolytic Properties of Leaves and Rhizomes of Schumannianthus dichotomus (Roxb.) Gagnep. Tropical Journal of Natural Product Research (TJNPR), 8(9), 8474-8481. https://doi.org/10.26538/tjnpr/v8i9.33

References

Radha SP, Pundir A. Review on ethnomedicinal plant: Trillium govanianum Wall. Ex D. Don. Int. J. Theor. Appl. Sci. 2019; 11:4-9.

Veldkamp JF and Turner IM. The correct name for Schumannianthus dichotomus (Marantaceae). Kew Bull. 2016; 71:1-4.

Barbhuiya AH and Ismail K. Effect of fiber length and loading on the properties of Schumannianthus dichotomus (murta) fiber–reinforced

epoxy composites. Int J Polym Anal Charact. 2016; 21(3):221-227.

Endale HT and Mengistie TA. ROS induced lipid peroxidation and their role in ferroptosis. Front Cell Dev Biol. 2023; 11:1226044.

Zohora FT, Islam SN, Khan SA, Hasan CM, Ahsan M. Antioxidant, cytotoxic, thrombolytic and antimicrobial activity of Zanthoxylum rhetsa root bark with two isolated quinolone alkaloids. Pharmacol Pharm. 2019; 10(3):137-145.

Noor S, Prodhan A, Zohora FT, Tareq FS, Ahsan M, Hasan CM, Islam SN. Phytochemical, antioxidant, antimicrobial, thrombolytic as well as cytotoxic studies on the stem bark of Manilkara zapota (sapotaceae). Asian J Chem. 2014; 26(18):6138.

Abo-Dola M, Lutfi M, Bakhiet A, Mohamed A. Anti-inflammatory, analgesic, antipyretic and the membrane-stabilizing effects of Tamarix

aphylla ethanolic extract. Eur J Med Plants. 2015; 5(4):341-348.

Ali M, Akhter R, Narjish SN, Shahriar M, Bhuiyan MA. Studies of preliminary phytochemical screening, membrane stabilizing activity,

thrombolytic activity and in-vitro antioxidant activity of leaf extract of Citrus hystrix. Int J Pharm Sci Res. 2015; 6(6):2367.

Tania UH, Hassan MR, Eshita NJ, Akhter R, Shahriar M. Evaluation of in vitro antioxidant and in vivo pharmacological activity of leaf extracts of Hoya parasitica (Wall.). J Appl Pharm Sci. 2016; 6(5):163-170.

Shahriar M, Akhter S, Hossain MI, Haque MA, Bhuiyan MA. Evaluation of in vitro antioxidant activity of bark extracts of Terminalia arjuna. J Med Plant Res. 2012; 6(39):5286-5298.

Adebayo SA, Ondua M, Shai LJ, Lebelo SL. Inhibition of nitric oxide production and free radical scavenging activities of four South African medicinal plants. J Inflamm Res. 2019; 12:195-203.

Lorigooini Z, Sadeghi Dehsahraei K, Bijad E, Habibian Dehkordi S, Amini-Khoei H. Trigonelline through the attenuation of oxidative

stress exerts antidepressant-and anxiolytic-like effects in a mouse model of maternal separation stress. Pharmacology. 2020;105(5-6):289-299.

Zhang Y, Shen Y, Zhu Y, Xu Z. Assessment of the correlations between reducing power, scavenging DPPH activity and anti-lipidoxidation capability of phenolic antioxidants. LWT-Food Science and Technology. 2015; 63(1):569-574.

Laboni FR, Mahmud S, Karim S, Das S, Shahriar, M., 2017. Biological investigations of different leaf extracts of Litsea liyuyingi (Family-Lauraceae). Iosr J. Pharm. and Biol. Sci, 12, pp.08-17.

Shahriar M, Khair N, Sheikh Z, Chowdhury S, Kamruzzaman M, Bakhtiar M, Chisty S, Narjish S, Akhter R, Akter N. Phytochemical

analysis, cytotoxic and in vitro antioxidant activity of Erythrina variegate bark. Eur J Med Plants. 2016; 11(3):1-5.

Manukumar HM and Umesha S. Assessment of membrane stabilizing activity from honey. An in-vitro approach. Acta Sci Pol Technol Aliment. 2015; 14(1):85-90.

e Silva PE, de Barros RC, Albuquerque WW, Brandão RM, Bezerra RP, Porto AL. In vitro thrombolytic activity of a purified fibrinolytic enzyme from Chlorella vulgaris. J Chromatogr B. 2018; 1092:524-529.

Jahan N, Shaari K, Islam SN, Azam AZ, Ahsan M. Chemical and Biological Profiling of Three Ferulic Acids Alkyl Esters Isolated from Jatropha pandurifolia (Family: Euphorbiaceae) Stem Bark. Biomed. Pharmacol. J. 2023 Jun 30;16(2):817-826.

Tung NT, Quan NV, Anh NP, Phuong NV, Hung NQ. Preliminary Phytochemical Evaluation and In Vitro Xanthine Oxidase Inhibitory

Activity of Balanophora subcupularis PC Tam and Balanophora tobiracola Makino (Balanophoraceae). Trop J Nat Prod Res. 2019; 3(1):6-9.

Mfotie Njoya E, Munvera AM, Mkounga P, Nkengfack AE, McGaw LJ. Phytochemical analysis with free radical scavenging, nitric oxide inhibition and antiproliferative activity of Sarcocephalus pobeguinii extracts. BMC Complement Altern Med. 2017; 17:1-9.

Shahriar M, Hossain I, Sharmin FA, Akhter S, Haque MA, Bhuiyan MA. In Vitro antioxidant and free radical scavenging activity of Withania Somnifera root. Iosr J Pharm. 2013; 3(2) 38-47.

Canabady-Rochelle LL, Harscoat-Schiavo C, Kessler V, Aymes A, Fournier F, Girardet JM. Determination of reducing power and metal

chelating ability of antioxidant peptides: Revisited methods. Food Chem Adv. 2015; 183:129-135.

Kupina S, Fields C, Roman MC, Brunelle SL. Determination of total phenolic content using the Folin-C assay: Single-laboratory validation,

first action 2017.13. J AOAC Int. 2018; 101(5):1466-1472.

Matić P, Sabljić M, Jakobek L. Validation of spectrophotometric methods for the determination of total polyphenol and total flavonoid

content. J AOAC Int. 2017; 100(6):1795-1803.

Kamath SD, Arunkumar D, Avinash NG, Samshuddin S. Determination of total phenolic content and total antioxidant activity in locally consumed foodstuffs in Moodbidri, Karnataka, India. Adv Appl Sci Res. 2015; 6(6):99-102.

Sheidu AR, Umar ZA, Abubakar A, Ahmed CB, Garba MM, Ogere AI, Murtala SO. Antioxidant and Hepatoprotective Potentials of

Methanol Extract of Ficus platyphylla Stem Bark (Moraceae) in Wistar Rats. Trop J Nat Prod Res. 2019; 4(3):91-97.

Manukumar HM and Umesha S. Assessment of membrane stabilizing activity from honey. An in-vitro approach. Acta Sci Pol Technol Aliment. 2015; 14(1):85-90.

Tabassum F, Chadni SH, Mou KN, Hasif KI, Ahmed T, Akter M. In-vitro thrombolytic activity and phytochemical evaluation of leaf extracts of four medicinal plants of Asteraceae family. J Pharmacogn Phytochem. 2017; 6(4):1166-1169.