Phytoconstituents Screening and Analysis of Antioxidant, Membrane Stabilizing, and Thrombolytic Properties of Leaves and Rhizomes of Schumannianthus dichotomus (Roxb.) Gagnep
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Abstract
The initial screening of various leaf and rhizome extracts of Schumannianthus dichotomus (Roxb) indicated the presence of saponins, phenols, flavonoids, glycosides, phytosterols, and tannins. To verify the therapeutic potential of this plant, known locally as Shital Pati, the antioxidant activity of the different extracts from its leaves and rhizomes were evaluated using eight methodologies. Using a Soxhlet extraction device, solvents like methanol, ethanol, and chloroform were used to extract the powdered leaves and rhizomes of this plant. The membrane stabilizing and thrombolytic potential of the extracts were also assessed using the clot lysis method. In the DPPH
scavenging test, the ethanol extracts of the leaf and rhizome showed the highest levels of free radical scavenging activity (IC50 22.54 and 19.78 μg/mL, respectively), compared to the standard (IC50 19.51 μg/mL). In the H2O2 scavenging assay, the chloroform leaf extract and methanol rhizome extract exhibited the highest inhibition with IC50 values of 2.03 and 1.85 µg/mL, respectively, compared to the standard L-Ascorbic acid at 1.69 µg/mL. The leaf and rhizome extracts displayed promising potential in total antioxidant, total phenolic content, and cupricreducing antioxidant capacity. The results indicated that all leaf and rhizome extracts have significant potential for membrane stabilizing activity compared to the standard. The methanol leaf and ethanol rhizome extracts of S. dichotomus have demonstrated better thrombolytic activity with 23.74±10.71 and 23.56±1.65% clot lysis, respectively, compared to the standard
streptokinase at 24.14±9.5%. This investigation confirms the therapeutic potential of S. dichotomus's leaves and rhizome.
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