Biological Properties And GC-MS Identification of Compounds of Ethanol Extracts and Volatile Oils From Citrus sinensis, Citrus paradisi and Citrus reticulata

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Folasade B. Oluwatobi
Olakunle B. Afolabi
Pius A. Okiki
Oghenerobor B. Akpor

Abstract

The numerous health advantages of citrus fruits are generally well-known. Therefore, this study investigated bioactive properties of ethanol extracts and volatile oils of three citrus fruits (orange, grape and tangerine) wastes. The antioxidant activities of the extracts and volatile oils were assessed using the 2, 2-diphenylpicrylhydrazyl (DPPH) radical scavenging test, while the antimicrobial qualities were determined using agar-well diffusion method. Gas chromatographymass spectrometry (GC-MS) was used to identify the chemicals constituents. The sample peel extracts exhibited effective inhibition against certain bacterial isolates, according to the results of the antibacterial assay. The mycelial inhibitory activity of all the citrus fruit waste extracts against Corynespora sp. was significantly higher (60–84%) than that of any other fungal pathogens under investigation. All the extract exhibited significant (p < 0.05) increases in antioxidant activity in a concentration-dependent manner. Bioactive compounds with known antibacterial properties were identified in the extracts' GC-MS fingerprints. 1,2-benzenedicarboxylic acid and diisooctyl ester were prevalent in grapes, accounting for 22.9% in pomace, 21.64% in seed, 15.07 percent in peel, and 23.15% in volatile oil, respectively. However, in the tangerine samples, n-hexadecanoic acid was the most prevalent compound with peel (25.42%) and seed (23.31%) extracts, and (+)- spathulenol (22.30%) being the most dominant in the volatile oil. In contrast, n-hexadecanoic acid was most dominant in the orange samples with percent composition in pomace (20.94%), seed (21.00%), peel (21.07%), and volatile oil (19.71%). Thus, these findings revealed that the citrus fruits wastes could be alternative sources of bioactive ingredients with antimicrobial and antioxidant potentials.

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How to Cite
Oluwatobi, F. B., Afolabi, O. B., Okiki, P. A., & Akpor, O. B. (2024). Biological Properties And GC-MS Identification of Compounds of Ethanol Extracts and Volatile Oils From Citrus sinensis, Citrus paradisi and Citrus reticulata. Tropical Journal of Natural Product Research (TJNPR), 8(9), 8453-8460. https://doi.org/10.26538/tjnpr/v8i9.30
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How to Cite

Oluwatobi, F. B., Afolabi, O. B., Okiki, P. A., & Akpor, O. B. (2024). Biological Properties And GC-MS Identification of Compounds of Ethanol Extracts and Volatile Oils From Citrus sinensis, Citrus paradisi and Citrus reticulata. Tropical Journal of Natural Product Research (TJNPR), 8(9), 8453-8460. https://doi.org/10.26538/tjnpr/v8i9.30

References

Amutha R, Kavusik T, Sudha A. Analysis of bioactive compounds in citrus fruit peels. Int J Sci Res. 2017; 6:19-27.

Bayram B, Ozkan G, Kostka T, Capanoglu E, Esatbeyoglu T. Valorization and application of fruit and vegetable wastes and by-products for food packaging materials. Molecules. 2021; 26(13):4031.

Abayomi OJ, Oyewole AO, Olagunju JA, Akinola OO, Omotosho OE. Curative effect of aqueous seed extract of Citrus paradisi against carbon tetrachloride-induced nephrotoxicity in Wistar rats. Trop J Nat Prod Res. 2021; 5(4):749-752.

Eneke IC, Essien EB, Wegu MO. Gas chromatography-Mass spectrometry (GC-MS) analysis of bioactive components present in grape citrus peel in Nigeria. GSC Adv Res Rev. 2021; 08(01):166–174.

Saini RK, Ranjit A, Sharma K, Prasad P, Shang X, Gowda KGM, Keum YS. Bioactive compounds of citrus fruits: A review of composition and health benefits of carotenoids, flavonoids, limonoids, and terpenes. Antioxidants. 2022; 11(2):239.

Dosoky NS and Setzer WN. Biological activities and safety of Citrus spp. volatile oils. Int J Mol Sci. 2018; 19(7): 1966.

Afroja S, Falgunee FN, Jahan MM, Akanda KM, Mehjabin S, Parvez GMM. Antibacterial activity of different citrus fruits. Specialty J Med Res Health Sci. 2017; 2(1): 25-32.

Sania N, Naveed A, Javeed A, Nasir MA, Attarad A, Muhammad Z. Management of citrus waste by switching in the production of nanocellulose. IET Nanobiotechnol. 2016; 1-5.

Khanal L, Sharma K, Pokharel Y, Kalauni S. Assessment of phytochemical, antioxidant and antimicrobial activities of some medicinal plants from Kaski district of Nepal. Am J Plant Sci. 2020; 11:1383-1397.

Mahcene Z, Khelil A, Hasni S, Akman PK, Bozkurt F, Birech K, Goudjil MB, Tornuk F. Development and characterization of sodium alginate based active edible films incorporated with volatile oils of some medicinal plants. Int J BiolMacromol. 2020; 145:124-132.

Association of Official Analytical Chemists. Official Method of Analysis. AOAC. 2004; 7: 56-132.

Javed S, Javaid A, Mahmood Z, Javaid A, Nasim F. Biocidal activity of citrus peel volatile oils against some food spoilage bacteria. Int J Enol Vitic. 2020; 7(7): 001-005.

Odebode AC, Mdachi SJ, Joseph CC, Irungu BN. Antibacterial activities of constituents from Isolona cauliflora and Cleistochlamyskirkii. Tanz J Sci. 2003; 29(2):19-26.

Ferdous N, Rahman M, Alamgir AN. Investigation on phytochemical, cytotoxic and antimicrobial properties of ethanolic extracts of Centella asiatica (L.) Urban J Med Plants Stud. 2017; 5:187-188.

Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Gen Pharmacol: Vascul Syst. 1999; 32(6):661-667.

Oluwatobi F, Afolabi O, Okiki P, Adeniyi F, Akpor O. Bioactive properties of the extracts of peels, pomace, seeds, and essential oils of Citrus limon and Citrus aurantifolia. J Appl Biol Biotechnol. 2024; 12(1):182-188.

Afolabi OB, Olasehinde OR, Owolabi OV, Jaiyesimi KF, Adewumi FD, Idowu OT, Mabayoje SO, Obajuluwa AO, Akpor OB. Insight into antioxidant-like activity and computational exploration of identified bioactive compounds in Talinum triangulare (Jacq.) aqueous extract as potential cholinesterase inhibitors. BMC Compl Med Ther. 2024; 24(1):134.

Subba MS, Somithri TC, Suryanarayana RAO. Antioxidant and antibacterial activities of Nephelium lappaceum I. Extracts. Food Sci Technol. 2008; 46:2029-2035.

Ahmed M, Alaa MA, Najwa MJAA. Study the effect of some citrus peel extracts against plant pathogenic fungi. Asian J Water Environ Pollut. 2022; 19(6): 103-110.

Cheng YJ, Wu YJ, Lee FW, Ou LY, Chen CN, Chu YY, Kuan YC. Impact of storage condition on chemical composition and antifungal activity of pomelo extract against Colletotrichum gloeosporioides and anthracnose in postharvest mango. Plants. 2022; 11(15): 2064.

Momodu IB, Okungbowa ES, Agoreyo BO, Maliki MM. Gas chromatography–mass spectrometry identification of bioactive compounds in methanol and aqueous seed extracts of azanza garckeana fruits. Nig J Biotechnol. 2022; 38(1):25-38.

Olasehinde OR, Afolabi OB, Owolabi OV, Akawa AB, Omiyale OB. GC–MS analysis of phytochemical constituents of methanolic fraction of Annona muricata leaf and its inhibition against two key enzymes linked to type II diabetes. Sci Afr. 2022; 16: 01178.