Isolation and Characterization of Anti-Inflammatory Compounds from <i>Radix bupleuri</i>

Quoc Hung Dinh; Xuan Phong Pham; Thi Tuyet Nhung Tran; Duy Nhat  Lai; Xuan Minh Ha; Thi Thu Hien Tran; Nam Trung Trinh; Van Thu Nguyen; Van Anh Cao

doi:10.26538/tjnpr/v8i8.43

Authors

Quoc Hung Dinh Military Institute of Traditional Medicine, Ha Noi 100000, Vietnam
Xuan Phong Pham Military Institute of Traditional Medicine, Ha Noi 100000, Vietnam
Thi Tuyet Nhung Tran Military Institute of Traditional Medicine, Ha Noi 100000, Vietnam
Duy Nhat Lai Military Institute of Traditional Medicine, Ha Noi 100000, Vietnam
Xuan Minh Ha Military Institute of Traditional Medicine, Ha Noi 100000, Vietnam
Thi Thu Hien Tran Military Institute of Traditional Medicine, Ha Noi 100000, Vietnam
Nam Trung Trinh Institute of Pharmaceutical Education, Vietnam Military Medical University, Hanoi 100000, Vietnam
Van Thu Nguyen Institute of Pharmaceutical Education, Vietnam Military Medical University, Hanoi 100000, Vietnam
Van Anh Cao Institute of Pharmaceutical Education, Vietnam Military Medical University, Hanoi 100000, Vietnam

DOI:

https://doi.org/10.26538/tjnpr/v8i8.43%20

Keywords:

Anti-inflammatory, Phenol, Saikosaponin, Radix bupleuri

Abstract

Radix bupleuri is traditionally used in Vietnam for its hepatoprotective activity. The herb was mainly imported from China, until it was recently introduced Vietnam. The plant is found mainly in the Northern region of Vietnam. The aim of this research was to isolate and characterize anti-inflammatory compounds from Radix bupleuri. The ethanol (EtOH) extract of Radix bupleuri was phytochemical investigation and the structure of five isolated known compounds was identified by 1D and 2D NMR data as well as by comparison with data reported in literature. The isolatd compounds were evaluated for their inhibitory effect on NO production in RAW 264.7 cells and the compounds showed moderate activity. Therefore, Radix bupleuri could serve as a potential therapeutic herb for the treatment of diseases related to inflammation.

Views | PDF Download | EPUB Download: 78 / 40 / 0

References

Yang F, Dong X, Yin X, Wang W, You L, Ni J. Radix bupleuri: A Review of Traditional Uses, Botany, Phytochemistry, Pharmacology, and Toxicology. BioMed Res Int. 2017; 2017, 7597596. Doi: 10.1155/2017/7597596.

Wang Y, Guo Q, Cheng Z, Zeng K, Liang H, Tu P, Chen S, Zhang Q. New saikosaponins from the roots of Bupleurum chinense. Phytochem Lett. 2017; 21: 183-189. Doi: https://doi.org/10.1016/j.phytol.2017.06.005

Yuan B, Yang R, Ma Y, Zhou S, Zhang X, Liu Y. A systematic review of the active saikosaponins and extracts isolated from Radix bupleuri and their applications. Pharm Biol. 2017; 55,(1): 620-635. Doi: 10.1080/13880209.2016.1262433

Nguyen HT, Nguyen Thi TH, Braire J, Dang Thi TA, Nguyen Van T. Microwave-Assisted Multicomponent Synthesis of New 6-Arylated 5-Hydroxy-benzo[a]phenazine Derivatives and Their Potential Anti-inflammatory Activity. ChemistrySelect. 2023; 8, (1): e202204376. Doi: https://doi.org/10.1002/slct.202204376

Si-Qi L, Long-Ze LA, Cordell G. Saikosaponin derivatives from Bupleurum wenchuanense. Phytochem. 1993; 33, (5): 1197-1205. Doi: https://doi.org/10.1016/0031-9422(93)85049-W

Nakahara Y, Okawa M, Kinjo J, Nohara T. Oleanene Glycosides of the Aerial Parts and Seeds of Bupleurum falcatum and the Aerial Parts of Bupleurum rotundifolium, and Their Evaluation as Anti-hepatitis Agents. Chem Pharm Bull. 2011; 59, (11): 1329-1339. Doi: 10.1248/cpb.59.1329

Zhang HM, Wang CF, Shen SM, Wang GL, Liu P, Liu ZM, Wang YY, Du SS, Liu ZL, Deng ZW. Antioxidant Phenolic Compounds from Pu-erh Tea. Molecules 2012; 17, (12): 14037-14045. Doi: 10.3390/molecules171214037

Ma T, Sun Y, Wang L, Wang J, Wu B, Yan,T, Jia Y. An Investigation of the Anti-Depressive Properties of Phenylpropanoids and Flavonoids in Hemerocallis citrina Baroni. Molecules 2022; 27, (18): 5809. Doi: 10.3390/molecules27185809