Morpho-Genetic Relatedness Among Wild and Cultured Clarias gariepinus (Buchell, 1822) Collected from Ogbomoso, South-western Nigeria.

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Ogundiran M. Akinloye
Fawole O. Olubanjo
Olanipekun S. Abimbola
Oduoye T. Olusola
Adebayo E. Adegoke
Majolagbe N. Olusola
Amao J. Ayobami
Balogun H. Adewale
Oladele V. Folashade

Abstract

Clarias species used in aquaculture are suffering from inbreeding depression with its attendant poor reproductive attributes and there is the need to explore the natural population to improve reproductive performance. Therefore, this work aimed at evaluating the morpho-genetic variability among wild and cultured Clarias garipinus available in Ogbomoso, South-Western, Nigeria. Fifty C. gariepinus samples were obtained from the wild and cultured populations and only ten were favoured for this work. The DNA was extracted from the caudal peduncles of five representatives of the two sampled populations and DNA amplification was done using cytochrome oxidase region’s COX1. Predictive Analytics Software version 20.0 was used for data analyses. Results showed that virtually all morphometric parameters measured were significantly higher in the cultured populations compared to the wild except the dorsal fin length (p=0.346). The A260/280 ratio ranges from 1.85 (C1) to 2.11 (W2), with most samples having a ratio close to 2.0, indicating high-purity DNA, which is around standard recommended DNA concentration for the amplification. The DNA amplified at 700 bp from a portion of mitochondrial DNA (mtDNA) of wild and cultured Clarias gariepinus. The genetic distance between each strain is represented by the length of the branch that connects them. Therefore, these suggest that C. gariepinus breeders and farmers should source C. gariepinus species from the wild in order to genetically enrich the gene pool. Additionally, head region-based morphometric traits might be more informative in distinguishing wild and cultured populations of C. gariepinus.

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How to Cite
Akinloye, O. M., Olubanjo, F. O., Abimbola, O. S., Olusola, O. T., Adegoke, A. E., Olusola, M. N., Ayobami, A. J., Adewale, B. H., & Folashade, O. V. (2024). Morpho-Genetic Relatedness Among Wild and Cultured Clarias gariepinus (Buchell, 1822) Collected from Ogbomoso, South-western Nigeria. Tropical Journal of Natural Product Research (TJNPR), 8(8), 8176-8182. https://doi.org/10.26538/tjnpr/v8i8.39
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How to Cite

Akinloye, O. M., Olubanjo, F. O., Abimbola, O. S., Olusola, O. T., Adegoke, A. E., Olusola, M. N., Ayobami, A. J., Adewale, B. H., & Folashade, O. V. (2024). Morpho-Genetic Relatedness Among Wild and Cultured Clarias gariepinus (Buchell, 1822) Collected from Ogbomoso, South-western Nigeria. Tropical Journal of Natural Product Research (TJNPR), 8(8), 8176-8182. https://doi.org/10.26538/tjnpr/v8i8.39

References

Salgotra RK, Chauhan BS. Genetic Diversity, Conservation, and Utilization of Plant Genetic Resources. Genes

(Basel). 2023; 14:1:174-181. doi: 10.3390/genes14010174

Raffard A, Santoul F, Cucherousset J, Blanchet S. The community and ecosystem consequences of intraspecific diversity: A meta-analysis. Biol Rev. 2019;94:648–661. doi: 10.1111/brv.12472

Davies ID, Cary GJ, Landguth EL, Lindenmayer DB, Banks SC. Implications of recurrent disturbance for genetic diversity. Ecol and Evol. 2016; 6:1181–1196

Dwivedi AK, Dubey VK. Advancements in morphometric differentiation: A review on stock identification among fish populations. Rev Fish Biol Fish. 2013; 23:1:23-39

Adah PM, Onyia LU, Obande RA. Fish Hybridization in Some Catfishes: A Review. Biotechnology. 2014;13; 6:248–251. https://doi.org/10.3923/biotech.2014.248.251

Agbolade O. Nazri A. Yaakob R. Ghani AA. Cheah YK. Morphometric approach to 3D soft-tissue craniofacial analysis and classification of ethnicity, sex, and age. PLoS ONE. 2020; 15:4: 231-242 https://doi.org/10.1371/journal.pone.0228402

Ogunbode TO. Ifabiyi PI. Rainfall trends and its implications on water resources management: a case study of Ogbomoso city in Nigeria. Int J Hydro. 2019; 3:210-215. DOI: 10.15406/ijh.2019.03.00182

Teugels GG. A systematic revision of the African species of the genus Clarias (Pisces; Clariidae). Annals Mus r. Afr Centr Tervuren (Sc. Zool). 247, 1986: 199 pp., 96

Abdel-Latif A. Osman G. Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize. Plant Meth. 2017; 13:1-9. https://doi.org/10.1186/s13007-016-0152-4

Solomon S. Okomoda V. Ogbenyikwu A. Intraspecific morphological variation between cultured and wild Clarias gariepinus (Burchell) (Clariidae, Siluriformes). Arch of Pol Fish. 2015;23. https://doi.org/10.1515/aopf-2015-0006

Brraich O. Akhter S. Morphometric characters and meristic counts of a fish, Crossocheilus latius latius (Hamilton-Buchanan) from Ranjit Sagar Wetland, India. Inter Jour of Fish and Aqua Stud. 2015; 45:66-72. https://www.semanticscholar.org/paper/Morphometric-characters-and-meristic-Counts-of-a-Brraich-Akhter/419939b6d48957df88647075113357e16a2307b2

Gonzalez-Martinez A. De-Pablos-Heredero C. González M. Rodriguez J. Barba C. García A. Usefulness of discriminant analysis in the morphometric differentiation of six native freshwater species from Ecuador. Animl. 2021;11:111. https://doi.org/10.3390/ani11010111

Agbolade O. Ojo A. Adedeji O. Oguntola J. Comparative analysis of size-related traits between wild and cultured fish populations. J Aqua Bio. 2024; 29:3:156-165

Ikpeme EV. Udensi OU. Okolo MC. Ogban FU. Ufford NG. Odo EU. Asuquo BO. Genetic relatedness of Clarias gariepinus (L.) from cultured and wild populations using multivariate analyses. Asi Jour of Ani Sci. 2016;10(2):131–138. https://doi.org/10.3923/ajas.2016.131.138

Langer S. Tripathi NK. Khajuria B. Morphometric and meristic study of Golden Mahseer, Tor putitora from Jhajjar Stream (J & K), India. Res J Anim Vet Fish Sci. 2013; 1(7):1-4

Wright J. Lee S. Zaikova E. Walsh D. Hallam S. DNA extraction from 0.22 μM Sterivex filters and cesium chloride density gradient centrifugation. Jour. of Vis. Expt. 2009; 31:10.3791/1352

Aboul-Maaty NAF. Oraby HAS. Extraction of high-quality genomic DNA from different plant orders applying a modified CTAB-based method. Bulletin of the National Research Centre. 2019; 43(1):25. https://doi.org/10.1186/s42269-019-0066-1

Joseph LJ. Setting up a laboratory. In: Weiss RE. Refetoff S. editors. Genetic Diagnosis of Endocrine Disorders. Academic Press; 2010. p. 303–314. https://doi.org/10.1016/B978-0-12-374430-2.00027-4

Setiati N. Partaya P. Hidayah N. Widiyaningrum P. Polymerase Chain Reaction-Amplification of COI Gene of Stingray and Shark Using Four Primer Pairs. Trop J Nat Prod Res. 2023; 7(7):3285-3291

Watson SA. McStay GP. Functions of cytochrome c oxidase assembly factors. Int Jour of Mol Sci. 2020; 21(19):7254. https://doi.org/10.3390/ijms21197254

Timón-Gómez A. Nývltová E. Abriata LA. Vila AJ. Hosler J. Barrientos A. Mitochondrial cytochrome c oxidase biogenesis: Recent developments. Seminars in Cell & Developmental Biology. 2018; 76:163–178. https://doi.org/10.1016/j.semcdb.2017.08.055

Rodrigues MS. Morelli KA. Jansen AM. Cytochrome c oxidase subunit 1 gene as a DNA barcode for discriminating Trypanosoma cruzi DTUs and closely related species. Par & Vect. 2017; 10(1):488. https://doi.org/10.1186/s13071-017-2457-1

Baum DA, Smith SD. Donovan SSS. The tree-thinking challenge. Science. 2005; 310(5750):979–980. https://doi.org/10.1126/science.1117727

Suleiman IO. Moruf RO. Usman BI. Population genetic structure of feral and cultured African catfish (Clarias gariepinus) inferred from random amplified polymorphic DNA in Kano, Nigeria. Trop J Nat Prod Res. 2023; 7(3):2650–2654. http://www.doi.org/10.26538/tjnpr/v7i3.27