Assessment of the Antioxidant Capacity and Cytotoxic Activity of <i>pomoea pes-tigridis</i>
DOI:
https://doi.org/10.26538/tjnpr/v8i8.5Keywords:
MTT assay, Cytotoxicity, Antioxidant, Phytochemical profile, Ipomoea pes-tigridisAbstract
Cancer, one of the deadliest diseases, is the second leading cause of mortality all over the world. The study aims to investigate the phytochemical profile and in vitro antioxidant activity of ethanol extracts from Ipomoea pes-tigridis and assess its cytotoxicity against three cell lines. Its qualitative and quantitative phytochemical analysis revealed that the plant possesses various secondary
metabolites like tannins and flavonoids, which have potent antioxidant properties. The DPPH (2,2-Diphenyl-1-picrylhydrazyl) and nitric oxide (NO) free radical assays showed promising in vitro antioxidant potential. The ethanol extracts demonstrated significant cytotoxicity against A375, B16-F10, and NHDF cell lines, with IC50 values ranging from 12.02 ± 3.14 μg/mL to 136.42 ± 2.92 μg/mL. These findings suggest that Ipomoea pes-tigridis may contain compounds with potential anticancer properties, which warrant further investigation for identification and characterisation. The observed activities of the plant can be credited to the polyphenolic compounds, and future research is needed to assess the detailed phytochemistry and biological activities of Ipomoea pestigridis.
References
Dokuparthi SK, Reddy TRM. Antioxidant and Nephroprotective Activity of Flavonoid Rich Fraction of Alphonsea sclerocarpa Thw. Int J Pharm Sci Drug Res. 2021; 13(4):384-394. DOI: 10.25004/IJPSDR.2021.130404
Sudheer Kumar D, Rama Mohan Reddy T. Antioxidant activity and hepatoprotective potential of flavonoid rich content of Alphonsea sclerocarpa Thw. Leaves. Int J Pharm Res. 2021; 13(3): 1309-1318.
Singamaneni V, Dokuparthi SK, Banerjee N, Kumar A, Chakrabarti T. Phytochemical investigation and antimutagenic potential of ethanolic extracts of Emblica officinalis, Terminalia chebula and Terminalia bellirica. The Nat Prod J. 2020; 10(4):488-94.2019; 9(1):1-7.
Muz B, de la Puente P, Azab F, Azab AK. The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia (Auckl). 2015; 3:83-92. doi: 10.2147/HP.S93413.
Gao X, Liu J, Cho KB, Kedika S, Guo B. Chemopreventive Agent 3,3′-Diindolylmethane Inhibits MDM2 in Colorectal Cancer Cells. Int J Mol Sci. 2020; 21(13):4642. https://doi.org/10.3390/ijms21134642.
Siegel RL, Miller KD, Wagle NS, Jemal A. Cancer statistics 2023. CA Cancr J Clin. 2023; 73:17– 48.
Nikolaou V, Stratigos AJ. Adjuvant treatment in advanced melanoma: How far have we come? JEADV. 2023; 37(5):851-852. 10.1111/jdv.19010.
Yu M, Gouvinhas I, Rocha J, Barros AIRNA. Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources. Sci Rep. 2021; 11(1):10041. doi: 10.1038/s41598-021-89437-4.
Okolie NP, Falodun A, Oluseyi D. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennettia Tripetala) and its potential for the inhibition of Lipid peroxidation. Afr J Trad Complement Altern Med 2014; 11(3):221-227.
Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, FalodunA. Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphyta jamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.
Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014; 10(4):177. doi: 10.3389/fphar.2013.00177.
Sandhya S, Sravanthi E, Vidya Vinod KR, Gouthami G, Saikiran M, Banji David. Alkaloids and Flavonoids of Aerial Parts of Ipomoea pes-tigridis (Convolvulaceae) are Potential Inhibitors of Staphylococcus epidermidis and Propionibacterium acnes. J Herbs Spices Med Plants. 2012); 8(4):370-386.
Anaha VI, Nithin Manohar R, Reshma BV. A review on Ipomoea pes-tigridis. World J Pharm Res. 2018; 7(10Spec.Iss.):1-10.
Selvam NT, Acharya MV. Review of Ipomoea pes-tigridis L.: traditional uses, botanical characteristics, chemistry and biological activities. Int J Pharm Sci Res. 2015; 6(12): 1443- 1448.
Karunakar S, Sujatha E, Anuradha S, VanithaN. Phytochemical and in vitro anti-inflammatory screening of Indigofera species. IJBPAS. 2021; 10(9):672-681. Doi:10.31032/IJBPAS/2021/10.9.1052
Sujatha E, Veena BS, Sowmyya T. The antioxidant and antihypertensive potential of Ledebouria hyderabadensis. Int J Health Sci. 2022; 6(S2):12617–12628. Doi: 10.53730/ijhs.v6nS2.8907.
Abbagoni S, Edupuganti S, Rani G. Phytochemical and antioxidant screening of Cocculus hirsutus and Calycopteris floribunda. Int J Health Sci. 2021;(S1): 576–584. https://doi.org/10.53730/ijhs.v5nS1.13642
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants (Basel). 2019; 8(4):96.
Broadhurst RB, jones WT. Analysis of condensed tannins using acidified vanillin. J Sci Food Agric. 1978; 48(3):788–794
Alam, Md Nur et al. Review on in vivo and in vitro methods evaluation of antioxidant activity.” Saudi Pharm J. 2013; 21(2):143-52.
Marcocci JJ. Marguire MT. Droy-lefaiz L. The nitric oxide scavenging properties of Ginkgo biloba extract Biochem. Biophys. Res. Commun., 1994;201: 748-755
Pravalika Kuchana, Sujatha E. Phytochemical Investigation, Antioxidant and Cytotoxic Potential of Dracaena reflexa Lam. IJBPAS. 2021; 10(9):698-708.
Lourenço SC, Moldão-Martins M, Alves VD. Antioxidantsof Natural Plant Origins: From Sources to Food Industry Applications. Molecules. 2019; 24(22):4132. doi: 10.3390/molecules24224132.
Rahman MM, Islam MB, Biswas M. et al. In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes. 2015; 8:621. https://doi.org/10.1186/s13104-015- 1618-6
Pola KK & Rada SK. Acute Dermal Toxicity Study of Acacia concinna Pods Extract in Wistar Rats. Trop J Nat Prod Res. 2023; 7(7):3398–3401.
Rada SK and Kusuma A. Acute and Sub-Acute Toxicity Studies of Starch Hyaluronate in Wistar Rats. Trop J Nat Prod Res. 2023;7(5):2965–2968.
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