Olive Cake Prevents Inflammation, Modulates Glomerulopathy, Glomerular Filtration Rate and Improves Renal Functions in Adult Obese Rats Fed a High-Fat Diet Since Weaning

Mansourou Samba Garba; Sherazede Bouderbala; Omar Kharoubi

doi:10.26538/tjnpr/v8i8.4

Authors

Mansourou Samba Garba Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Life and Natural Sciences, University Oran1 Ahmed Ben Bella, Oran 31100, Algeria
Sherazede Bouderbala Laboratory of Clinical and Metabolic Nutrition, Department of Biology, Faculty of Life and Natural Sciences, University Oran1 Ahmed Ben Bella, Oran 31100, Algeria
Omar Kharoubi Laboratory of Biotoxicology Experimentale, Biodepollution and Phytoremediation, Department of Biology, Faculty of Life and Natural Sciences, University Oran1 Ahmed Ben Bella, Oran 31100, Algeria.

DOI:

https://doi.org/10.26538/tjnpr/v8i8.4

Keywords:

Morphometric, Inflammation, Redox status, Olive cake, Obesity-related kidney injury, Rat

Abstract

Cleistocalyx operculatus is ubiquitous and known by folk experiences for its antimicrobial properties, thus promising as a low-cost green pesticide. In this work, the plant ethanol extract was investigated against Cercospora nicotianae, a pathogenic fungus implicated in the leaf spot in Pennywort. Characterisation of the plant extract showed 21 identifiable constituents (1-21), with the major components: 4’,5'-dimethoxy-2'-hydroxy-4-methylchalcone (21; 41.71 %), phytol (15; 8.46 %), and 5.7-dimethoxyflavanone (20; 5.20 %). The in vitro antifungal screening of the extract revealed potent inhibitory capacity up to 7 days after inoculation (concentration = 9.54 mg.mL^-1). The inhibitory potential was predicted by docking simulation, i.e., 19-Q6DQW3 ( -10.7 kcal.mol^-1; -12.7 kcal.mol^-1) > 21-Q6DQW3 ( -10.3 kcal.mol^-1; -11.9 kcal.mol^-1) > 20-Q6DQW3 ( -10.3 kcal.mol^-1_;-11.7 kcal.mol^-1). The chemical potentiality was derived from quantum calculation, i.e., 22 (-1172.77 a.u.) > 21 ≈ 19 (approx. -1000 a.u.) > 20 (-ca. -950 a.u.) for chemical stability, and 3 (3.40 Debye), 15 (2.47 Debye), and 5 (2.03 Debye) for physical compatibility. The suitability for biological and pesticidal applications was justified by physicochemical analyses. The results revealed a firm correlation between the extracted major constituents (20 and 21) and the total antifungal activity against C. nicotianae, thus encouraging its local usage.

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