Phytochemical Profiling and GC- MS Analysis of Lantana camara Leaf Extract
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Abstract
Identification of the phytochemical composition of plants is vital in new drug discovery and lead compound development for the management of infectious and non-infectious diseases. Lantana camara is an important medicinal essential oil-producing plant used in folklore medicine for the treatment of several diseases. This study evaluated the phytochemical composition and profiled the biologically active principles present in L. camara leaf extracts collected from Orba, Enugu State of Nigeria. The profiling of L. camara leaves methanol and aqueous extracts was done using qualitative and quantitative phytochemical and GC-MS techniques. The phytochemical screening showed the presence of alkaloids, terpenoids, flavonoids and other phenolic compounds. There were no significant differences (p > 0.05) in the steroids, saponins, terpenoids, glycosides and phenolic contents of methanol and aqueous extracts. The phenolic content in methanol (1563.85±0.07 mg/100g) and aqueous (1425.23±0.36 mg/100g) were significantly higher than other phytochemicals tested in this study. There was significantly (p < 0.05) higher alkaloids (506.74±5.52 mg/100g), flavonoids (372.04±8.39 mg/100g) and tannins (1426.43±7.43 mg/100g) in the methanol compared with the aqueous extracts with 349.92±13.10 mg/100g, 231.99±6.16 mg/100g and 1351.62±20.6 mg/100g respectively. The GC-MS analysis showed the presence of 39 biologically important compounds which included bis(2-ethylhexyl) phthalate, 9,19-cyclolanostan-3-ol-11-one acetate, 4H-1-benzopyran-4-one-5-hydroxy-2-(4-hydroxyphenyl), n-hexadecanoic acid, (Z)-7-hexadecenal, phytol acetate, phytol, 3,7,11,15-tetramethyl-2-hexadecen-1-ol and bicyclo [4.4.0] dec-2-ene-4-ol 2-methyl-9-(prop-1-en-3-ol-2-yl). The phytochemical analysis and the GC-MS profiling of the extracts of L. camara showed the presence of biologically important phytochemicals which could be responsible for the ethnomedicinal effects of L. camara.
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References
Ayoka TO, Nnadi CO. Lesser-known leafy vegetables of Southeastern Nigeria (Vitex doniana and Zanthoxylum zanthoxyloides). Not Sci Biolo. 2022; 14(2): 11777.
Nnadi CO, Ngwu SO, Ohagwu MBZ. In-vivo and in-silico evidence of antitrypanocidal activities of selected plants from Asteraceae family against Trypanosoma brucei brucei. Bioint Res Appl Chem. 2023; 13(1): 30.
Ayoka TO, Nwachukwu N, Ene AC, Igwe CU, Nnadi CO. Hepatocurative and histopathological evaluations in albino rats exposed to Vitex doniana alkaloids. Lett Appl NanoBioSci. 2023; 12(2): 56
Okorie NH, Ali IJ, Nnadi CO, Ani DI. Anti-ulcer activity of crude extract and fractions of Acanthus montanus (Nees) T. Anderson on indomethacin-induced ulcer. Trop J. Nat Prod Res. 2023; 7(5): 3017-3021.
Ayoka TO, Nwachukwu N, Ene AC, Igwe CU, Nnadi CO. The hepatocurative effects of Zanthoxylum zanthoxyloides alkaloids on tetrachloromethane-induced hepatotoxicity on albino rats. Indian J. Clin Biochem. 2024; 39(2): 186-197.
Odoemelam EI, Ugorji CO, Ezema EE, Agbo MO, Nnadi CO, Orjiocha SI, Okonkwo VI, Nwafor FI, Ugwu GN, Chukwuma MO. Estimation of total phenolics, total flavonoids content and in vitro antioxidant activities of extract and fractions of Asplenium platyneuron (Carl Linnaeus). Trop J. Nat Prod Res. 2024; 8(3): 6723-6730.
Yusuf ATM, Shaikat MAM, Bhuiya MAM, Dutta M, Nath AK, Dash PR. Utilizing the medicinal properties of Delonix regia for diseases and beyond: a review of its phytochemistry and pharmacology. Trop J Nat Prod Res. 2023; 7(12): 5355-5365.
Priyanka N, Joshi PK. A review of Lantana camara studies in India. Int J Sci Res Pub. 2013; 3(10): 42-52.
Vardien W, Richardson DM, Foxcroft LC, Thompson GD, Wilson JR, Le Roux JJ. Invasion dynamics of Lantana camara L. (sensu lato) in South Africa. S Afr J Bot. 2012; 81: 81-94.
Zheng H, Wei N, Wang L, He P. Effects of Lantana camara leaf extract on the activity of superoxide dismutase and accumulation of H2O2 in water hyacinth leaf. J Plant Physiol Mol Bio. 2006; 32(2): 189-194.
Jyothi MJ, Vamsi S, Satish C, Nagaveni K. Lantana camara Linn: a review. Int J Phytother. 2012; 2(2): 66-73.
Bhuvaneswari E, Giri RS. Physicochemical and phytochemical screening in Lantana camara leaves. J Pharmacogn Phytochem. 2018; 7(6): 1962-1966.
Ganjewala D, Sam S, Khan KH. Biochemical compositions and antibacterial activities of Lantana camara plants with yellow, lavender, red and white flowers. Eurasia J Biosci. 2009; 3(1): 69-77.
Ganesh T, Sen S, Thilagam E, Thamotharan G, Loganathan T, Chakraborty R. Pharmacognostic and anti-hyperglycemic evaluation of Lantana camara (L.) var. aculeate leaves in alloxan-induced hyperglycemic rats. Int J Res Pharm Sci. 2010; 1(3): 247-252.
Pour BM, Srinivasan S, Jegathambigai RN, Suresh R. A comparative study: antimicrobial activity of methanol extracts of Lantana camara various parts. Pharmacogn Res. 2009; 1(6): 348-351.
Ahmad F, Rather MA, Siddiqui MA. Nematicidal activity of leaf extracts from Lantana camara L. against Meloidogyne incognita (Kofoid and White) chitwood and its use to manage roots infection of Solanum melongena L. Braz Arch Biol Technol. 2010; 53(3): 543-548.
Ogba OM, Nyambi ES, Olorode OA, Asemota E. Antibacterial activity of Lantana camara montevidensis leaf extract on wound isolates using animal models. Trop J Nat Prod Res. 2021; 5(12): 2160-2164.
Thamotharan G, Sekar G, Ganesh T, Sen S, Chakraborty R, Kumar SN. Antiulcerogenic effects of Lantana camara Linn. leaves on in vivo test models in rats. Asian J Pharm Clin Res. 2010; 3(3): 57-60.
Sathish R, Vyawahare B, Natarajan K. Antiulcerogenic activity of Lantana camara leaves on gastric and duodenal ulcers in experimental rats. J Ethnopharmacol. 2011; 134(1):195-197.
Falodun A, Siraj R, Choudhary MI. GC-MS Analysis of insecticidal leaf essential oil of Pyrenacantha staudtii Hutch and Dalz (Icacinaceae). Trop J Pharm Res. 2009; 8(2): 139-143.
Sousa EO, Rocha JB, Barros LM, Barros AR, Costa JG. Phytochemical characterization and in vitro antioxidant properties of Lantana camara L. and Lantana montevidensis Briq. Ind Crops Prod. 2013; 43: 517-522.
Bashir S, Jabeen K, Iqbal S, Javed S, Naeem A. Lantana camara: phytochemical analysis and antifungal prospective. Planta Daninha. 2019; 37: e019193526.
Nnadi CO, Okorie HN, Nwodo NJ. Evaluation of in vitro antiprotozoal and cytotoxic Activities of selected medicinal plants used in Nigerian folk medicine. Trop J. Nat Prod Res. 2021; 5(4): 609-612.
Udeh NE, Nnadi CO, Anaga AO, Asuzu IU. Bioactivity-guided fractionation of a methanol leaf extract from Gnetum africanum with potential anti-diabetic activity: (-)- 24.Epicatechin as the active principle. J. Res Pharm. 2021; 25(1): 72-79
Harborne JB. Textbook of phytochemical methods. A guide to modern techniques of plant analysis. (3rd ed.). London: Chapman and Hall Limited; 1998. 302 p.
Udeani TK, Ugwu LO, Nnadi CO, Okwuosa CN. Evaluation of anti-microbial activity and proximate composition of alkaloids from Vitex doniana seed. Dhaka Univ J. Pharm Sci. 2021; 20(1): 81-86.
Obonga WO, Omeje EO, Nnadi CO and Ocheme WG. Phytochemical evaluation of extracts and GC-MS analysis of oil from Monodora myristica seed. Dhaka Univ J. Pharm Sci. 2019; 18(1): 69-73.
Obonga WO, Nnadi CO, Okafor SN, Omeje EO, Igbinnaduwa P, Okoli CO, Osadebe PO (2019). Anti-inflammatory activity and accelerated stability studies of crude extract syrup of Cannabis sativa. Trop J. Pharm Res. 2019; 18(2): 295-301.
Ngassoum MB., Yonkeu S, Jirovetz L, Buchbauer G, Schmaus G, Hammerschmidt FJ. Chemical composition of essential oils of Lantana camara leaves and flowers from Cameroon and Madagascar. Flavour Fragr J. 1999; 14(4): 245-250.
Ayoka TO, Ngwu N, Ene AC, Igwe CU, Nnadi CO. Liquid Chromatography-Mass Spectrometric Analyses of Potential Antioxidant Constituents from Zanthoxylum zanthoxyloides Leaves: Probing into the Role of Alkaloids. Trop J. Nat Prod Res. 2020; 4(10): 817-823.
Nnadi CO, Ozioko LU, Eneje GC, Onah CM, Obonga WO. In-vivo antitrypanosomal effect and in-silico prediction of chronic toxicity of N-methylholaphyllamine in rats. Trop J. Pharm Res. 2020; 19(11), 2369-2375.
Nnadi CO, Onoja SO, Ezeh GC, Ikpa CB, Ezeja MI, Udem SC, Anaga AO, Asuzu IU. Antioxidant and antidiabetic potentials of a dimethoxylated flavone obtained from Helianthus annuus L. leaf extract. Pak J. Pharmacol. 2022; 37(2): 59-69.
Munhoz VM, Longhini R, Souza JR, Zequi JA, Mello EV, Lopes GC, Mello JC. Extraction of flavonoids from Tagetes patula: process optimization and screening for biological activity. Rev Bras Farmacogn. 2014; 24: 576-583.
Kalita S, Kumar G, Karthik L, Rao KVB. Phytochemical composition and in vitro hemolytic activity of Lantana camara L. (Verbenaceae) leaves. Pharmacologyonline. 2011; 2011(1): 59-67.
Patil SP, Kumbhar ST. Evaluation of terpene-rich extract of Lantana camara L. leaves for antimicrobial activity against mycobacteria using Resazurin Microtiter Assay (REMA). Beni-Suef Univ J Basic Appl Sci. 2018; 7(4): 511-515.
Obonga WO, Nnadi CO, Agbo MO, Kenechukwu FC, Nwodo U. (2014). Distribution of methicillin resistant Staphylococcus aureus (MRSA) in apparently healthy population and its susceptibility to saponins from Dialium guineense. Br J. Pharm Res (now J. Pharm Res Int). 2014; 4(18): 2200-2209.
Nnadi CO. Nwodo NJ, Kaiser M, Brun R, Schmidt TJ. Steroid alkaloids from Holarrhena africana with strong activity against Trypanosoma brucei rhodesiense. Molecules. 2017; 22(7): 1129.
Khan H, Pervaiz A, Intagliata S, Das N, Nagulapalli Venkata KC, Atanasov AG, Najda A, Nabavi SM, Wang D, Pittalà V, Bishayee A. The analgesic potential of glycosides derived from medicinal plants. DARU J. Pharm Sci. 2020; 28: 387-401.
Tracanna MI, Amani SM, Romano E, Raschi AB, Molina LR, Piro OE, Castellano EE, Benavente AM. Crystal structure, spectroscopic properties and antimicrobial activity of 4h-1-benzopyran-4-one, 5-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-6, 8-dimethyl from Miconia ioneura Griseb. Melastomataceae. Mol Med Chem. 2010; 21: 94-104.
Huang Z, Lin Y, Fang J. Biological and pharmacological activities of squalene and related compounds: potential uses in cosmetic dermatology. Molecules. 2009; 14(1): 540-554.
Brigelius-Flohé R. Bioactivity of vitamin E. Nutr Res Rev. 2006; 19(2): 174-186.
Penduka D, Buwa L, Mayekiso B, Basson AK, Okoh AI. Identification of the antilisterial constituents in partially purified column chromatography fractions of Garcinia kola seeds and their interactions with standard antibiotics. Evid Based Complement Alternat Med. 2014; 2014: 850347.
Kumar D, Karthik M, Rajakumar R. GC-MS analysis of bioactive compounds from ethanolic leaves extract of Eichhornia crassipes (Mart) Solms. and their pharmacological activities. Pharma Innov J. 2018; 7(8): 459-462.
Zanatta N, Borchhardt DM, Alves SH, Coelho HS, Squizani AM, Marchi TM, Bonacorso HG, Martins MA. Synthesis and antimicrobial activity of new (4, 4, 4-trihalo-3-oxo-but-1-enyl)-carbamic acid ethyl esters, (4, 4, 4-trihalo-3-hydroxy-butyl)-carbamic acid ethyl esters, and 2-oxo-6-trihalomethyl-[1, 3] oxazinane-3-carboxylic acid ethyl esters. Bioorg Med Chem. 2006; 14(9): 3174-3184.
Mazzari S, Canella R, Petrelli L, Marcolongo G, Leon A. N-(2-hydroxyethyl) hexadecanamide is orally active in reducing edema formation and inflammatory hyperalgesia by down-modulating mast cell activation. Eur J Pharmacol. 1996; 300(3): 227-236.
Kim BR, Kim HM, Jin CH, Kang SY, Kim JB, Jeon YG, Park KY, Lee IS, Han AR. Composition and antioxidant activities of volatile organic compounds in radiation-bred Coreopsis cultivars. Plants. 2020; 9(6): 717.
Santos CC, Salvadori MS, Mota VG, Costa LM, de Almeida AA, de Oliveira GA, Costa JP, de Sousa DP, de Freitas RM, de Almeida RN. Antinociceptive and antioxidant activities of phytol in vivo and in vitro models. Neurosci J. 2013; 2013: 949452.
Guerrero RV, Vargas RA, Petricevich VL. Chemical compounds and biological activity of an extract from Bougainvillea x Buttiana (var. rose) Holttum and Standl. Int J Pharm Pharm Sci. 2017; 9(3): 42-46.
Thomas JA, Thomas MJ, Gangolli SD. Biological effects of di-(2-ethylhexyl) phthalate and other phthalic acid esters. Crit Rev Toxicol. 1984; 13(4): 283-317.
Huang L, Zhu X, Zhou S, Cheng Z, Shi K, Zhang C, Shao H. Phthalic acid esters: natural sources and biological activities. Toxins. 2021; 13(7): 495.
Tan M, Zhou L, Huang Y, Wang Y, Hao X, Wang J. Antimicrobial activity of globulol isolated from the fruits of Eucalyptus globulus Labill. Nat Prod Res. 2008; 22(7): 569-575.
Nithya M, Ragavendran C, Natarajan D. Antibacterial and free radical scavenging activity of a medicinal plant Solanum xanthocarpum. Int J Food Prop. 2018; 21(1): 313-327.
Kohoude MJ, Gbaguidi F, Agbani P, Ayedoun M, Cazaux S, Bouajila J. Chemical composition and biological activities of extracts and essential oil of Boswellia dalzielii leaves. Pharm Biol. 2017; 55(1): 33-42.
Vyas S, Pandya D, Mankad A. A review on Prosopis cineraria as an important plant of arid regions of India. EPRA Int J Multidiscip Res. 2020; 6(3): 1-6.
Abubakar MN, Majinda RRT. GC-MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines. 2016; 3(1): 3.
Priya ES, Selvan PS, Tamilselvan R. Phytochemical investigation of Allophylus serratus Kurz leaves by UV and GC-MS analysis. Res J Phytochem. 2012; 6(1): 17-24.
Ambrosio CM, Diaz-Arenas GL, Agudelo LP, Stashenko E, Contreras-Castillo CJ, Da Gloria EM. Chemical composition and antibacterial and antioxidant activity of a57.citrus essential oil and its fractions. Molecules. 2021; 26(10): 2888.
Ibrahim EA, Wang M, Radwan MM, Wanas AS, Majumdar CG, Avula B, Wang YH, Khan IA, Chandra S, Lata H, Hada GM, Abdel Salam RA, Ibrahim AK, Ahmed SA, Elsohly MA. Analysis of terpenes in Cannabis sativa L. using GC/MS: method development, validation, and application. Plant Med. 2019; 85(5): 431-438.
Chan WK, Tan LT, Chan KG, Lee LH, Goh BH. Nerolidol: a sesquiterpene alcohol with multi-faceted pharmacological and biological activities. Molecules. 2016; 21(5): 529.
Akpuaka A, Ekwenchi MM, Dashak DA, Dildar A. Biological activities of characterized isolates of n-hexane extract of Azadirachta indica A. Juss (Neem) leaves. Nat Sci. 2013; 11(5): 141-147.
Premjanu N, Jaynthy C. Antimicrobial activity of diethyl phthalate: an in-silico approach. Asian J Pharm Clin Res. 2014; 7(4): 141-142.
Jahan I, Tona MR, Sharmin S, Sayeed MA, Tania FZ, Paul A, Chy MN, Rakib A, Emran TB, Simal-Gandara J. GC-MS phytochemical profiling, pharmacological properties, and in silico studies of Chukrasia velutina leaves: a novel source for bioactive agents. Molecules. 2020; 25(15): 3536