Ameliorative Effect of Pomegranate Molasses on Phenylhydrazine-Induced Anemia in Rats
Article Sidebar
Main Article Content
Abstract
Pomegranate molasses and peels contain minerals, antioxidants and several vitamins. The aim of this study was to investigate the effect of pomegranate molasses to protect against phenylhydrazine induced anemia in rats. 40 male rats were divided into four equal groups; a control group, Pomegranate molasses treated group, Phenylhydrazine (PHZ) treated group, Pomegranate molasses + Phenylhydrazine treated group. Hematology, hemoglobin electrophoresis, G6PD, Iron, and erythropoietin were evaluated. Spleen was obtained for histopathological examination. The results revealed a significant increase in reticulocytes, osmotic fragility, iron and erythropoietin with a significant decrease in Glucose-6-Phosphate Dehydrogenase in Phenylhydrazine group, compared with Control group. Co-administration of Pomegranate molasses with Phenylhydrazine showed significant decrease in reticulocytes, osmotic fragility, iron and erythropoietin and significant increase in Glucose-6-Phosphate Dehydrogenase compared with Phenylhydrazine group. It could be concluded that pomegranate molasses could improve hematological parameters and had anti-inflammatory effect on spleen.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Berger J.Phenylhydrazine haematotoxicity. J. Appl. Biomed (2007); 5: 125-130. DOI: 10.32725/jab.2007.017.
Akpinar-Bayizit A., Ozcan T., Yilmaz-Ersan L. and Yildiz E. (2016): Evaluation of Antioxidant Activity of Pomegranate Molasses by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Method. IJCEA..(2016); 7(1) 71:74. DOI:10.7763/IJCEA.2016.V7.545.
Hasan S.M.H.,Abou-Rawash A.E.A. and Bekheet M.S. Protective Role of an Aqueous Extract of Punica Granatum (Pomegranate) Peel on Lead-Induced Anemia In Rats Alex. J. Vet. Sci.. (2016); 50(1): 99-108. Doi: 10.5455/ajvs.228349.
Riaz A. and Khan R.A.Anticoagulant, antiplatelet and antianemic effects of Punica granatum (pomegranate) juice in rabbits. Blood Coagulation and Fibrinolysis. (2016); 27(3):287-93. doi: 10.1097/MBC.0000000000000415.
Feldman B.F., Zinkl J.G. and Jain N.C. Schalm's veterinary hematology, 5th edition. Lippincott Williams and Wilkins A Wolters Company Philadelphia, Baltimore, New York, London, (2000); pp: 1219-1224.
Mafuvadze B. and Erlwanger K.H."The Effect of EDTA, Heparin and Storage on The Erythrocyte Osmotic Fragility, Plasma Osmolality and Haematocrit of Adult Ostriches (Struthio camelus). Veterinarski arhiv (2007); 77(5): 427-434. https://hrcak.srce.hr/24763.
Owren P.A.Congenital hemolytic jaundice. the pathogenesis of the ˝hemolytic crisis˝. Blood. (1948); 3(3): 231-248. https://doi.org/10.1182/blood.V3.3.231.231.
Schneider R.G.Laboratory Identification of the Hemoglobins, Lab Management. (1981); 29-43.
Schreiber W.E.Iron and Porphyrin Metabolism. Clinical Chemistry: Theory Analysis, Correlation, 5th Ed., Kaplan, L.A., Pesce, A.J., (Mosby, Inc.). (2010); 755 and appendix.
Ciniselli C.M., De Bortoli M., Taverna E., Varinelli L., Pizzamiglio S., Veneroni S., Bonini C., Orlandi R., Verderio P. and Bongarzone I. Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone. Expert Review of proteomics (2015); 12(6): 695-701. doi: 10.1586/14789450.2015.1099436.
Rodak B.F.Diagnostic Hematology. Philadelphia, PA: W.B. Saunders Company., (1995); 218.
Bancroft J.D. and Gamble M.Theory and practice of histological technique. 4th edition. Churchil Livingston, New York, London, San Francisco, Tokyo, (2008).
Quintero D., Ancel T., Cassie G., Ceron R. and Darwish A. "Workload Optimized Systems: Tuning POWER7 for Analytics";.(2013).
Mubaraki M.A., Hafiz T.A., Dkhil M.A. and Al-Quraishy S.Beneficial effect of Punica granatum peel extract on murine malaria-induced spleen injury. CAM – NCI, (2016); 16: 221. doi: 10.1186/s12906-016-1207-9.
Criswell K.A., Sulkanen A.P., Hochbaum A.F. and Bleavins M.R. Effects of phenylhydrazine or phlebotomy on peripheral blood, bone marrow and erythropoietin in Wistar rats. J. Appl. Toxicol. (2000) ; 20(1): 25-34. doi: 10.1002/(sici)1099-
(200001/02)20:1<25::aid-jat624>3.0.co;2-7.
Crosby W.H. The Hemolytic States. Bull. N. Y. Acad. Med. (1954) ; 30 (1): 27- 42.
Goheen S.C., Larkin E.C. and Rao G.A. Morphology and fatty acid composition of reticulocytes from phenylhydrazine-treated rats. Lipids. (1982) ;17(9):594-597.
Gordon A.S. Erythropoietin. In: Harris, R.S., Diczfalusy, E., Munson, P.L., Glover, J., eds. Vitamins and Hormones. New York, Academic Press. (1973) ;pp. 105-174.
Ashour T.H. Hematinic and anti-anemic effect of Thymoquinone against phenyl hydrazine-induced hemolytic anemia in rats. Res J Med Sci. (2014); 8(2):67-72. DOI: 10.36478/rjmsci.2014.67.72.
Elwej A., Ben Salah G., Kallel C., Fakhfakh F., Zeghal N. and Ben Amara I. Protective Effects of Pomegranate Peel against Hematotoxicity, Chromosomal Aberrations, and Genotoxicity induced by Barium Chloride in Adult Rats. Pharm.Biol..
(2016);54(6):96474.doi:10.3109/13880209.2015.1087035.
Achraf A.Hamdi C., Turki M., Abdelkarim O., Ayadi F., Hoekelmann A., Yaich S. and Souissi N.Natural pomegranate juice reduces inflammation, muscle damage and increase platelets blood levels in active healthy Tunisian aged men. Alexandria Med J. (2018);54(1):45-48.http://dx.doi.org/10.1016/j.ajme. 2017.03.005.
Kharchoufi S., Licciardello F., Siracusa L., Muratore G., Hamdi M. and Restuccia C. Antimicrobial and antioxidant features of ‘Gabsi & pomegranate peel extracts. Ind. Crops. Prod.(2018);111:345-352. https://doi.org/10.1016/j.indcrop.2017.10.037.
Aboelsoued D., Abo-Aziza F. A. M., Mahmoud M. H., Abdel Megeed K. N., Abu El Ezz N. M. T. and Abu-Salem F. M. Anticryptosporidial effect of pomegranate peels water extract in experimentally infected mice with special reference to some biochemical parameters and antioxidant activity.J.Parasit.Dis.(2019);43(2):215-228. https://doi.org/10.1007/s12639-018-01078-z.
Sood R.Clinical Haematology, Medical Laboratory technology methods and interpretations volume 1.Textbook sixth edition. (2009); Pg: 318-319.
Gamit M.J. and Talwelkar H.S. Survey of different types of anemia. Int. J. Med. Sci. Public Health. (2017); 6(3): 493-6. DOI: 10.5455/ijmsph.2017.0851807092016.
Gbolahan A. O., Victor A. S.A., Clara T. F., Wole A. O. and Adewunmi A. K. Ameliorative Effects of Aqueous Extract of Citrullus Lanatus Fruit on Phenylhydrazine Induced Anemia In Male Wistar Rats. A.J.V.S. (2020); 64 (1): 97-103. DOI: 10.5455/ajvs.39300.
Chauhan S.P., Sheth N.R. and Suhagia B.N. Hematinic effect of fruits of Opuntia elatior Mill. on phenylhydrazine-induced anemia in rats. AYU. (2015); 36(2): 208-13. doi: 10.4103/0974-8520.175549.
Gracious Ross R., Selvasubramanian S. and Jayasundar S. Immunomodulatory Activity of Punica granatum in Rabbits - A Preliminary Study. J. Ethnopharmacol. (2001); 78 (1): 85-7. doi: 10.1016/s0378-8741(01)00287-2.
Yamasaki M., Kitagawa T., Koyanagi N., Chujo H. , Maeda H.., Kohno-Murase J., Imamura J., Tachibana H., Yamada K. Dietary Effect of Pomegranate Seed Oil on Immune Function and Lipid Metabolism in Mice. Nutrition, (2006) ; 22 (1): 54-9. doi: 10.1016/j.nut.2005.03.009.
Doostan F., Vafafar R. Zakeri-Milani P., Pouri A., Afshar R.A., Abbasi M.MEffects of pomegranate (Punica granatum L.) seed and peel methanolic extracts on oxidative stress and lipid profile changes induced by methotrexate in rats. Adv. Pharm. Bull. (2017) ; 7:269. doi: 10.15171/apb.2017.032.
Aduwamai U.H., Abimbola M.M. and Ahmed Z.H. Effect of Solanum nigrum Methanol Leaf Extract on Phenylhydrazine Induced Anemia in Rats. Jordan J Biol Sci. (2018); 11(1): 65-71.
Jain S.K. and Subrahmanyam D. On the mechanism of phenylhydrazine-induced hemolytic anemia. Biochem Biophys Res Commun. (2004); 82(4): 1320-1324.
Stockham L. and Scott A. Fundamental of veterinary clinical pathology. Lowa State Press, first edition.. A Blackwell Puplishing Company., (2002); pp.87