Antimicrobial Potentials of Secondary Metabolites of Endophytic Fungi Isolated from Synclisia scabrida Miers
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Abstract
The increase in the development of multi-drug resistance among pathogens calls for the development of novel therapeutic agents with better effectiveness and low toxicity. The plant-associated endophytic fungi are producers of bioactive secondary metabolites with enormous potential. The study aimed to isolate, identify, and characterize the secondary metabolites of endophytic fungi isolated from different non-diseased plant parts (leaf, stem, and root bark) of Synclisia. scabrida Miers. The isolation of endophytic fungi was carried out using standard protocols and identified by molecular techniques. The bioactive secondary metabolites were extracted using ethyl acetate. The quantitative phytochemical screening of the fungal metabolites was carried out using Gas chromatography. The antimicrobial activities of ethyl acetate extracts of the isolates were analyzed via agar well diffusion technique and the minimum inhibitory concentrations (MICs) were determined by broth dilution method. The different endophytic fungal metabolites exhibited a good presence of different phytochemical compounds. The fungal metabolites showed antimicrobial activities against several of the six tested human pathogenic bacteria or fungi. Among these metabolites, L1, R1 and R2 metabolites were the most potent, exhibiting significant activities against the test organisms. L1 and R1 metabolites showed the lowest MIC of 0.53±0.13 and 0.70±0.07 mg/ml against A. niger and C. albicans respectively. A total of five endophytic fungi were isolated from the leaf, stem, and root bark of S. scabrida Miers, and three different fungal species were identified using molecular techniques. The plant, S. scabrida Miers harbors endophytic fungi yielding bioactive metabolites with potent antimicrobial activities against pathogenic microorganisms.
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References
Visalakchi S, Muthumary J. Antimicrobial activity of the new endophytic Monodictys castaneae SVJM139 pigment and its optimization. Afr J Microbiol Res. 2010; 4(9):38-44.
Tang KWK, Millar BC, Moore JE. Antimicrobial resistance (AMR). Brit J of Biomed Sci. 2023; 80:11387. https://doi.org/10.3389/bjbs.2023.11387
Llor C, Bjerrum L (2014). Antimicrobial resistance: Risk associated with antibiotic overuse and initiatives to reduce the problem. Therap Adv in Dru Saf. 2014; 5(6):229–241. https://doi.org/10.1177/2042098614554919
World Health Organization (WHO). Outlines 40 research priorities on antimicrobial resistance, n.d. Accessed on 27th March 2024.
Gouda S, Das, Sen S, Shin H, Patra JK. Endophytes: a treasure house of bioactive compounds of medicinal importance. Front in Microbio. 2016; 7. https://doi.org/10.3389/fmicb.2016.01538
Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbio and Mol Bio Rev. 2003; 67(4): 491–502. https://doi.org/10.1128/MMBR.67.4.491-502.2003
Specian V, Sarragiotto MH, Pamphile JA, Clemente E. Chemical characterization of bioactive compounds from the endophytic fungus Diaporthe helianthi isolated from Luehea divaricata. Brazil J of Microbio. 2012; 43(3): 1174–1182. https://doi.org/10.1590/S1517-838220120003000045
Stępniewska Z, Kuźniar A. Endophytic microorganisms—Promising applications in bioremediation of greenhouse gases. App Microbio and Biotech. 2013; 97(22):9589-9596. https://doi.org/10.1007/s00253-013-5235-9
Dudeja SS, Giri, R. Beneficial properties, colonization, establishment and molecular diversity of endophytic bacteria. Afri J of Microbio Res. 2014; 8:1562-1572. https://doi.org/10.5897/AJMR2013.6541
Bhandari V, Suresh A. Next-generation approaches needed to tackle antimicrobial resistance for the development of novel therapies against the deadly pathogens. Front in Pharmac. 2022; 13. https://www.frontiersin.org/articles/10.3389/fphar.2022.838092
Hashem AH, Attia MS, Kandil EK, Fawzi MM, Abdelrahman AS, Khader MS, Khodaira MA, Emam AE, Goma MA, Abdelaziz AM (2023).
Bioactive compounds and biomedical applications of endophytic fungi: A recent review. Microb Cel Facto. 2023; 22(1):107.
https://doi.org/10.1186/s12934-023-02118-x
Gunatilaka AAL. Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity, and implications of their occurrence. J of Nat Prod. 2006; 69(3): 509–526. https://doi.org/10.1021/np058128n
Onwudiwe TC, Ughachukwu PO, Unekwe PC, Ogamba JO. Evaluation of antiulcer properties of ethanolic and hot aqueous stem extracts of
Synclisia Scabrida on experimentally induced ulcer models in albino mice. Ann of Med and Healt Sci Res. 2012; 2(2):134-139 https://doi.org/10.4103/2141-9248.105660
Anowi FC, Onyekaba TU, Eze CC, Ike C, Ezenachi VC. Preliminary phytochemical investigations and evaluation of antimicrobial activity of n-hexane extract of the leaves of Synclisia scabrida family menispermaceae. Res J Pharmaceutical Sci. 2013; 2(3):1-5.
Afonne OJ, Orisakwe OE, Obi E, Orish C, Akumka DD. Some pharmacological properties of Synclisia scabrida Miers. India J Pharmacol. 2000; 32:239-241.
Okezie U, Eze P, Ajaghaku D, Okoye F, Esimone C. Isolation and screening of secondary metabolites from endophytic fungi of Vernonia amygdalina and Carica papaya for their cytotoxic activity. Plant Medic. 2015; 81(16):s-0035-1565554. https://doi.org/10.1055/s-0035-1565554
Maksun R. Molecular identification of endophytic fungi isolated from Garcinia porrecta and Garcinia forbesii. J Farma Indon. 2009; 4(4):156-160.
Wu J, Shen W, Sun W, Tueller P. (2002). Empirical patterns of the effects of changing scale on landscape metrics. Landsca Ecolo. 2002; 17:761-782. https://doi.org/10.1023/A:1022995922992
Kumar S, Kaushik N. Batch culture fermentation of endophytic fungi and extraction of their metabolites. Bio-protocol. 2013; 3(19). https://doi.org/10.21769/BioProtoc.926
Aida P, Rosa V, Blamea F, Tomas A, Salvaador C. Paraguyan plants used in traditional medicine. Shor Com. J. Ethnopharm. 2001: 16:93-98.
Deeksha S, Avijit P, Pavan KA. Evaluation of bioactive secondary metabolites from Endophytin fungus Pestalotiopsis neglecta-5510 isolated from leaves of Cupressus torulosa D. Don. 3 Biotech. 2016; 6:210.
Deepthi VC, Sumathi S, Athikkavil F, Elyas, K. Isolation and identification of endophytic fungi with antimicrobial activities from the leaves of Elaeocarpus sphaericus (gaertn.) k. Shum. and Myristica fragrans houtt. Intern J of Pharmaceu Sci and Res. 2018. https://doi.org/10.13040/IJPSR.0975-8232.9(7).2783-91
Bisht DR, Sharma D, Agrawal PK. Antagonistic and antibacterial activity of endophytic fungi isolated from needle of Cupressus torulosa D. Don. Asian J Pharm Clin Res. 2016; 9(3):282-288.
Thakur D, Sahani K (2019). Qualitative and quantitative phytochemical analysis of endophytic fungi (ef8; Aspergillus sp.3) isolated from Boerhavia diffusa l., stem. Asian J of Pharmaceut and Clin Res. 2019; 111-116. https://doi.org/10.22159/ajpcr.2019.v12i3.28242
Nurunnabi, T. R., Sabrin, F., Sharif, D. I., Nahar, L., Sohrab, Md. H., Sarker, S. D., Rahman, S. M. M., & Billah, Md. M. (2020). Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest. Ad. Trad. Med., 20(3), 419–425. https://doi.org/10.1007/s13596-019-00422-9
Bhardwaj A, Sharma D, Jadon N, Agrawal P. Antimicrobial and phytochemical screening of endophytic fungi isolated from spikes of Pinus rouxburghii. Archiv of Clin Microb. 2015; 6.
Devi NN, Prabaka JJ, Wahab F. Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica. Asian Pac J Trop Biomed. 2012; 2:1280-1284. https://doi.org/10.1016/S2221-1691(12)60400-6
Kumar V, Prasher IB. Phytochemical analysis and antioxidant activity of endophytic fungi isolated from Dillenia indica Linn. Appl Biochem and Biotech. 2024; 196(1):332–349. https://doi.org/10.1007/s12010-023-04498-7
Dzoyem J, Mcgaw L, Kuete V, Bakowsky U. Anti-inflammatory and Anti-nociceptive Activities of African Medicinal Spices and Vegetables. In: Medicinal Spices and Vegetables from Africa: Therapeutic Potential Against Metabolic, Inflammatory, Infectious and Systemic Diseases. 2017; 239-270. https://doi.org/10.1016/B978-0-12-809286-6.00009-1
Zerroug A, Sadrati N, Demirel R, Sabrina B, Harzallah D. Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L. Afr. J. Micr, Res,. 2018; 12:1056-1066. https://doi.org/10.5897/AJMR2018.8887
Munaganti, R. K., Muvva, V., Konda, S., Naragani, K., Mangamuri, U. K., Dorigondla, K. R., & Akkewar, Dattatray. M. (2016). Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards. Brazilian Journal of Microbiology, 47(4), 1030–1038. https://doi.org/10.1016/j.bjm.2016.07.010