Effect of African Leaves (Vernonia amygdalina Delile) on the Development of T47D Breast Cancer Cells
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Abstract
Cancer is still a health problem in the world. The number of sufferers and deaths from cancer
continues to increase from year to year, especially breast cancer. Cancer treatment is often
associated with high toxicity and low selectivity, and the emergence of resistance to
chemotherapeutic agents is a serious problem. Therefore, the development of new anticancer
agents is a priority. This research provides information regarding the potential of the Vernonia
amygdaline Delile plant for the development of breast cancer. Several studies show that this
plant has the potential to be used as an anticancer agent. The research was conducted by
extracting and fractionating this plant's leaves and then assessing the cytotoxic effect on T47D
cell viability using Microtetrazolium. Next, cell cycle inhibition, apoptosis, p53 and Akt
protein expression were analyzed using flow cytometry techniques. The results showed that
the dichloromethane fraction had the highest yield. The dichloromethane fraction had the best
IC50 value, 76.72 ± 1.79 μg/mL. Able to inhibit the cell cycle in the G2/M phase, stimulate
apoptosis, increase p53 protein expression and inhibit Akt protein expression in T47D cells.
The dichloromethane fraction can potentially be developed in treating breast cancer
development.
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