Extract and Fixed Oil of Moroccan Lavandula dentata Aerial Parts as Potential Antioxidants

Main Article Content

Abid Houssni
El Abdali Youness
Jalte Meryem
El battioui Fadoua
Omaima El Bouazzi
Allali Aimad
Mikou Karima

Abstract

Aromatic and medicinal plants constitute a vast source of bioactive compounds, with remarkable
chemical diversity. The aim of the present study is to investigate the antioxidant activity of
extracts and fixed oils of Lavandula dentata L. aerial parts. Dried powdered aerial parts of
Lavandula dentata was extracted with methanol by refluxing. Different solvent fractions of
aqueous methanol extract of L. dentata aerial part were prepared by successive extraction in
chloroform, ethyl acetate, and n-butanol in order of increasing polarity. Fixed oil of L. dentata
aerial parts was obtained by maceration in chloroform at room temperature. Total phenolic and
flavonoid contents of the crude methanol extract was determined using standard methods. The
antioxidant activity of the crude methanol extract, fractions, and fixed oil was evaluated using
the 2,2-diphenyl-1-picryl hydrazyl (DPPH) free radical scavenging assay. Total polyphenolic
and flavonoid contents determination revealed that L. dentata crude methanol extract have high
contents of polyphenols (27.43 mg GAE/g), and flavonoids (20.62 mg QE/g). The ethyl acetate
fraction of L. dentata exhibited the highest antioxidant activity with IC50 value of 16.41 ± 0.11
µg/mL as measured by the DPPH radical scavenging assay. The n-butanol extract also
demonstrated notable antioxidant activity with IC50 value of 42.32 ± 0.86 µg/mL. The fixed oil
extracted from L. dentata aerial parts demonstrated mild antioxidant activity, with IC50 of 414.2
mg/mL. The findings from this study have shown that Lavandula dentata aerial parts contains
bioactive compounds with antioxidant potentials.

Article Details

How to Cite
Houssni, A., Youness, E. A., Meryem, J., Fadoua, E. battioui, Bouazzi, O. E., Aimad, A., & Karima, M. (2024). Extract and Fixed Oil of Moroccan Lavandula dentata Aerial Parts as Potential Antioxidants. Tropical Journal of Natural Product Research (TJNPR), 8(7), 7704–7708. https://doi.org/10.26538/tjnpr/v8i7.12
Section
Articles
Author Biographies

Abid Houssni, Higher Institute of Nursing Professions and Health Techniques of Rabat - Ministry of Health and Social Protection – Morocco.

Biology and Health Laboratory, Faculty of Sciences, Ibn Tofail University, Kenitra, Morocco.

Allali Aimad, High Institute of Nursing Professions and Health Techniques, Fez, Morocco.

Laboratory of Plant, Animal, and Agro-industry Productions, Faculty of Sciences, University of Ibn Tofail, Kenitra, Morocco.

References

El Abdali Y, Mahraz AM, Beniaich G, Mssillou I, Chebaibi M,

Bin Jardan YA, Lahkimi A, Nafidi HA, Aboul-Soud MAM,

Bourhia M, Bouia A. Essential oils of Origanum compactum

Benth: Chemical characterisation, in vitro, in silico, antioxidant,

and antibacterial activities. Open Chem. 2023a; 21:20220282.

El Abdali Y, Beniaich G, Mahraz AM, El Moussaoui A, Bin

Jardan YA, Akhazzane M, Chebaibi M, Nafidi HA, Eloutassi N,

Bourhia M, Bouia A. Antibacterial, Antioxidant, and in silico

NADPH Oxidase Inhibition Studies of Essential Oils of

Lavandula dentata against Foodborne Pathogens. Evid Based

Complement Alternat Med. 2023b; 2023: 1-12.

Hmamouchi M. Moroccan medicinal and aromatic plants.

Traditional uses, Market, Biology, Chemistry, Pharmacology,

Toxicology, Lexicon. 2nd ed. Mohammedia: Fédala; 2001. 450

p.

Mikou K, Rachiq S, Oulidi AJ. Ethnobotanical survey of

medicinal and aromatic plants used by the people of Fez in

Morocco. Phytothér. 2016; 14(1):35-43.

Ben El Jilali S, Ihamdane R, Moubchir T, Beniaich G, Mssillou

I, El Abdali Y, Allali A, Khadmaoui A. Lavandula dentata

Essential Oils: A Bio-Insecticide for an Agroecological

Approach to Protecting Chickpea Seeds against Callosobruchus

maculatus. Trop J Nat Prod Res. 2023; 7(11):5123-5127.

Allali A, Bourhia M, Hadin H, Rezouki S, Salamatullah AM,

Soufan W, Hail ZR, Ouahmane L, El abdali Y, Eloutassi N,

Fadli M. Essential oils from Artemisia herba alba Asso.,

Maticaria recutita L., and Dittrichia viscosa L. (asteraceae): A

promising source of eco-friendly agents to control

Callosobruchus maculatus fab. Warehouse pest. J Chem. 2022;

:1-14.

Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N,

Boulaaba M, Abdelly C. Phenolic composition of Cynara

cardunculus L. organs, and their biological activities. C R Biol.

; 331(5):372-379.

El Abdali Y, Allali A, Agour A, Mikou K, Lahkimi A, Eloutassi

N, Bouia A. Phytochemical Screening, and in vitro Antiradical

and Immunostimulant Potential of Linum Usitatissimum L.

Pharmacol Online. 2021; 3:602-614.

Merghem R, Jay M, Viricel MR, Bayet C, Voirin B. Five 8-Cbenzylated flavonoids from Thymus hirtus (Labiateae).

Phytochem. 1995; 38(3):637-640.

Bettaieb RI, Bourgou S, Saidani TM, Fauconnier ML, Ksouri R.

Study of the chemical composition and antioxidant activity of

various extracts of Dentate Lavender (Lavandula dentata). J

New Sci Agric Biotech. 2017; 39(2):2096-2105.

Bachiri L, Echchegadda G, Ibijbijen J, Nassiri L. Phytochemical

Study and Antibacterial Activity of Two Species of Lavender

Native to Morocco: «Lavandula stoechas L. and Lavandula

dentata L.». Eur Sci J. 2016; 12:313.

Amaral JS, Valentão P, Andrade PB, Martins RC, Seabra RM.

Phenolic composition of hazelnut leaves: Influence of cultivar,

geographical origin and ripening stage. Sci Horticult. 2010;

(2):306–313.

Wang J and Mazza G. Effects of anthocyanins and other

phenolic compounds on the production of tumor necrosis factor

α in LPS/IFN-γ-activated RAW 264.7 macrophages. J Agric

Food Chem. 2002; 50(15):4183-4189.

Marambe PWMLHK, Shand PJ, Wanasundara JPD. An in‐vitro

investigation of selected biological activities of hydrolysed

flaxseed (Linum usitatissimum L.) proteins. J Am Oil Chem

Soc. 2008; 85(12):1155-1164.

Liu F, Liao S, Zou Y, Xiao G. Antioxidant activity of Phellinus

igniarius extracts (830.32). FASEB J. 2014; 28(1):830-832.

Belyagoubi-Benhammou N, Belyagoubi L, El Zerey-Belaskri A,

Zitouni A, Ghembaza N, Benhassaini H, Atik-Bekkara F, Piras

A, Falconieri D, Rosa, A. Fatty acid composition and

antioxidant activity of Pistacia lentiscus L. fruit fatty oil from

Algeria. J Food Meas Charact. 2018; 12:1408–1412.

Kaur P, Waghmare R, Kumar V, Rasane P, Kaur S, Gat Y.

Recent advances in utilization of flaxseed as potential source for

value addition. OCL. 2018; 25(3):A304.

Most read articles by the same author(s)