Evaluation of the Antioxidant, Antinociceptive and Anti-inflammatory Activities of Combretum nigricans Ethanol Leaf Extract
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Abstract
Combretum nigricans is a known medicinal plant employed in Nigerian ethnomedicine for the treatment of pain, inflammation and other ailments. This study aims at evaluating the antioxidant, antinociceptive and anti-inflammatory effects of C. nigricans ethanolic leaf extract.
The antioxidant activity of C. nigricans ethanolic leaf extract was evaluated in vitro using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) model, while acetic acid writhing test and formalin nociception test were used in evaluating the antinociceptive activity in mice. Formalin-induced inflammation was used to evaluate the anti-inflammatory effect in mice. HPLC analysis of the extract showed the presence of several compounds including ferulic acid. The extract exhibited good DPPH antioxidant activity. Its IC50 value was 0.0003971 mg/mL. Oral acute toxicity test showed the LD50 is greater than 2000 mg/kg. In the acetic acid test, all extract test doses (200 – 800 mg/kg) had significant inhibitory effect against abdominal writhes usually evoked by acetic acid. The different extract doses had antinociceptive effect against phases 1 and 2 pain. Also, the extract doses used for the study (200 – 800 mg/kg) had anti-allodynia effect with the 800 mg/kg dose having a better effect of 44.44 and 79.83 % on days 1 and 2 respectively. Furthermore, the extract demonstrated significant anti-inflammatory activity against formalin induced inflammation in the experimental animals. The study outcome shows that C. nigricans extract has good antioxidant, anti-nociceptive and anti-inflammatory effects.
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Pandelani FF, Nyalunga SLN, Mogotsi MM, Mkhatshwa VB. Chronic pain: its impact on the quality of life and gender. Front. Pain Res. 2023, 4:1253460.
Tompkins DA, Hobelmann JG, Compton P. Providing chronic pain management in the “fifth vital sign” era: historical and treatment perspectives on a modern-day medical dilemma. J Drug Alc Dep 2017;173(1):S11–21.
Castroman P, Quiroga O, Mayoral Rojals V, Gómez M, Moka E, Pergolizzi JJ, Varrassi G. Reimagining How We Treat Acute Pain: A Narrative Review. Cureus. 2022;14(4):e23992.
Megwas AU, Akuodor GC, Chukwu LC, Aja DO, Okorie EM, Ogbuagu EC, Eke DO, Chukwumobi AN. Analgesic, anti-inflammatory and antipyretic activities of ethanol extract of Annona senegalensis leaves in experimental animal models. Int J Basic Clin Pharmacol 2020;9(10):1477 – 1484.
Sofidiya MO, Ikechukwu JU, Nnah VE, Olaleye OO, Basheeru K, Sowemimo AA, Ajayi AM. Anti-inflammatory and antinociceptive activities of Daniellia oliveri (Fabaceace) stem bark extract. J Ethnopharmacol 2023;2(309):116337.
Gias ZT, Afsana F, Debnath MP, Alam S, Ena TN, Hossain HM, Jain P, Reza MH. A mechanistic approach to HPLC analysis, antinociceptive, anti-inflammatory and postoperative analgesic activities of Panch phoron in mice. BMC Complement Med Ther 2020;20(102):1-9
Chinedu E, Okhale SE. Ethnomedicinal, phytochemical and pharmacological review of Asclepidiaceae. J Prev Diagnos Treat Strateg Med 2023;2:3-18.
Chinedu E, Akah PA, Ofili CC, Agatemor UM, Ameh SF, Jacob DL,Onah AI. Evidence supporting the use of Combrertum nigricans as an antimalarial agent in ethnomedicine. Int J Current Resear Physio Pharmacol 2021;5(2):13-20.
Handa SS, Khanuja SPS, Longo G, Rakesh DD. Extraction technologies for medicinal and aromatic plants. International Centre for Science and High Technology ICS-UNIDO, Trieste, Italy; 2008.
Evans WC. Trease and Evans Pharmacognosy, (14th ed), WB Saunders Company Limited, London;2005:357-358.
NIH. Guide for the care and use of Laboratory animal (Revised). Washington: NIH Publication; 1985:83-123.
Moriasi G, Ireri A, Ngugi MP. In Vitro Antioxidant Activities of the Aqueous and Methanolic Stem Bark Extracts of Piliostigma thonningii (Schum). J Evid Based Integr Med. 2020; 25: 2515690X20937988. doi:10.1177/2515690X20937988.
Organization for Economic Co-operation and Development OECD guidelines for the testing of chemicals Organization for Economic Co-operation and Development, Paris (2002) [Online] Available from: http://www.oecd.org/chemicalsafety/testing/2741541.pdf [Accessed on 27th November, 2023]
Woode E, Kukuia KKE, Ameyaw EO, Mante PK, Adongo DW. Screening of Central Effects of the Leaves of Ananas comosus (Geiseler) Mull. Arg. in Mice. Pharmacologia 2012;3(12): 683-692.
Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory Pain. 1987;30(1):103–14.
Simões RR Kraus IS, Rosso R, Bridi A, Casoti R, Dahmer J, Morel FA, dos Santos SRA, Zanchet ME. Root bark of Discaria americana attenuates pain: A pharmacological evidence of interaction with opioidergic system and TRP/ASIC channels. J Ethnopharmacol 2018; 227: 258-267.
Xuanbin Wang, Yan Ma, Qihe Xu, Alexander N. Shikov, Olga N. Pozharitskaya, Elena V. Flisyuk, Meifeng Liu, Hongliang Li, Liliana Vargas-Murga, Pierre Duez, Flavonoids and saponins: What have we got or missed? Phytomed 2023;109:1-10.
Chetehouna S, Derouiche S, Réggami Y, Boulaares I, Frahtia A. Gas Chromatography Analysis, Mineral Contents and Anti-inflammatory Activity of Sonchus maritimus. Trop J Nat Prod Res. 2024;8(4):6787-98.
Christianty FM, Dewi AS, Khasanah F, Holidah D, Dewi IP, Fajrin FA. Drug-Piperaceae Herb Interaction Potency Through Analgesic, Anxiolytic, and Anti-Inflammatory Activity Studies. Trop J Nat Prod Res. 2024; 8(4):6781-6786
Bektas N, Arslan R.The Centrally-Mediated Mechanisms of Action of Ferulic Acid–InducedAntinociception. Marmara Pharm J 2016; 20: 303-310.
Zduńska K, Dana A, Kolodziejczak A, Rotsztejn H. Antioxidant Properties of Ferulic Acid and Its Possible Application. Skin Pharmacol Physiol. 2018;31(6):332-336.
Liu Y, Shi L, Qiu W, Shi Y. Ferulic acid exhibits anti-inflammatory effects by inducing autophagy and blocking NLRP3 inflammasome activation. Mol Cell Toxicol. 2022;18(4):509-519.
Rodwattanagul S, Nimlamool W, Okonogi S. Antioxidant, antiglycation, and anti-inflammatory activities of Caesalpinia mimosoides. Drug Discov Ther 2023;17(2):114-123.
Mothana AAR, Al-Said SM, Al-Rehaily JA, Thabet MT,. Awad AN, Lalk M, Lindequist U. Anti-inflammatory, antinociceptive, antipyretic and antioxidant activities and phenolic constituents from Loranthus regularis Steud. ex Sprague. Food Chem 2012;130:344–349.
Woode E. Albizia zygia (DC.) J.F. Macbr. (Leguminosae-Mimosoideae) root extract exhibits anti-nociceptive and antipyretic activities in murine models. J Ethnopharmacol 2017; 6(199):183-193.
Kou J, Ni Y, Li N, Wang J, Liu L, Jiang ZH. Analgesic and anti-inflammatory activities of total extract and individual fractions of Chinese medicinal plant Polyrhachis lamellidens. Bio Pharmaceut Bulletin 2005;28:176–180.
Ameyaw EO, Woode E, Boakye‑Gyasi E, Abotsi WK, Kyekyeku JO, Adosraku RK. Anti-allodynic and Anti-hyperalgesic effects of an ethanolic extract and xylopic acid from the fruits of Xylopia aethiopica in murine models of neuropathic pain. Phcog Res 2014;6:172‑9.