Isolation, Carbohydrate Metabolism Profile, and Molecular Identification of Plant-Derived Lactic Acid Bacteria from Musa acuminata x balbisiana and Medinilla cummingii Naudin
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Abstract
Plants serve as a conducive milieu for diverse microorganisms, including lactic acid bacteria (LAB). Heightened concerns surrounding issues related to dairy products have catalyzed interest in exploring plant-derived LAB as valuable reservoirs for innovative strains with applications in both industrial and medical domains. The regional variability of LAB species and the lack of documented isolation studies in Mindanao, Philippines, especially concerning endemic flowers, are noteworthy factors. Consequently, the primary aim of this study is to systematically isolate, purify, characterize, and identify LAB originating from two plant sources: Musa acuminata x balbisiana and Medinilla cummingii Naudin. The two LAB isolates denoted as BNA1 and MED1, respectively, manifest the cardinal attributes indicative of lactic acid bacteria, namely gram-positive, catalase-negative, coccobacilli in morphology, and display proficient clearing around the colony through lactic acid production on MRS agar media supplemented with 1% calcium carbonate (CaCO3). Using the API 50 CHL system to study phenotypic characteristics through sugar fermentation showed that BNA1 has vigorous fermentation activity on 27 carbohydrates and weak fermentation activity on 14 carbohydrates. Meanwhile, MED1 has demonstrated robust fermentation activity on 21 carbohydrates. Based on the 16S rRNA gene, BNA1 and MED1 are identified as MED1 as Lactiplantibacillus sp. (GenBank Accession Number PP627039) and Enterococcus faecium (GenBank Accession Number PP627037), respectively. The findings of this research will serve as a fundamental basis for future investigations, particularly on its potential as new probiotic strains from plants and their corresponding applications.
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