Ethanol Leaf Extract of Acrostichum aureum Modifies Carbon Tetrachloride-induced Oxidative Stress and Hepato-renal Damage in Rats
DOI:
https://doi.org/10.26538/tjnpr/v8i6.8Keywords:
Histopathology, Oxidative stress, CCl4, Acrostichum aureum, Kidney, LiverAbstract
Liver and kidney diseases are public health concerns due to ineffectiveness and adverse effects of orthodox therapeutics. Consequently, medicinal plants are becoming attractive as plausible source of protective agents against hepatorenal toxicities. The protective effect of ethanol leaf extract of Acrostichum aureum (ELAA) was examined in a CCl4 rat model of hepato-renal injury. Forty-eight adult male Sprague–Dawley rats were divided into eight treatment groups (n=6 each). Treatment lasted for 21 days and the groups included: a negative control that received olive oil, a positive control group that received quercetin (10 mg/kg/day), two groups that received ELAA (50 or 100 mg/kg/day) alone, a CCl4 (1 ml/kg twice a week) treated-group and three CCl4-cotreatment groups that received 50 mg/kg/day ELAA or 100 mg/kg/day ELAA or quercetin with CCl4. On the 22nd day, blood was collected and used for evaluation of hepato-renal health markers including alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, creatinine, urea, total protein, albumin, Na+, and Cl-. Rats were sacrificed and malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels were determined in the liver and kidney after recording their weights. Compared to the negative control, CCl4 caused significant (p<0.05) increases in organ weight, organo-somatic indices, ALT, AST, bilirubin, and creatinine, while Na+ and Cl- were significantly decreased. Marked elevation of MDA coupled with decreased SOD and GPx activities were also recorded following CCl4 treatment. Supplementation with ELAA reversed the parameters toward control values similarly to quercetin. Suggesting that ethanol extract of Acrostichum aureum ameliorated CCl4-induced hepato-renal injury by counteracting oxidative stress.
References
Asrani SK, Devarbhavi H, Eaton J, Kamath PS. Burden of liver diseases in the world. J Hepatol. 2019;70 (1):151-171.
Luyckx VA, Tonelli M, Stanifer JW. The global burden of kidney disease and the sustainable development goals. Bull World Health Org. 2018;96(6):414-422D. doi:10.2471/BLT.17.206441
Unsal V, Cicek M, Sabancilar İ. Toxicity of carbon tetrachloride, free radicals and role of antioxidants. Rev 3.Environ Health 2021; 36 (2):279-95. https://doi.org/10.1515/reveh-2020-0048
Lixin X, Erli G, Songping H, Yonggen Z, Wang J, Lijun Y. Yi guan jian, a traditional Chinese herbal medicine, alleviates carbon tetrachloride-induced liver injury. eCAM. 2019:9824728.
Acheampong DO, BLixin X, Erli G, Songping H, Yonggen Z, Wang J, Lijun Y. Yi guan jian, a traditional Chinese herbal medicine, alleviates carbon tetrachloride-induced liver injury. eCAM. 2019:9824728.
Eze GI, Akonoafua KA. Effects of ethanol leaf extract of Lawsonia inermis Linn. on carbon tetrachloride-induced liver injury in adult Wistar rats.Trop J Nat Prod Res 2019; 3(8):252-260
Alkinani KB., Ali EMM, Al-Shaikh TM, Khan JAA, Al-naomasi TM, Ali SS, Abduljawad AA, Mosa OF, Zafar TA. Hepatoprotective effects of (−) epicatechin in CCl4-induced toxicity model are mediated via modulation of oxidative stress markers in rats, eCAM 2021;4655150.
Jin S, Li H, Han M, Ruan M, Liu Z, Zhang F, Zhang C, Choi Y, Liu B: Mesenchymal stem cells with enhanced Bcl-2 expression promote liver recovery in a rat model of hepatic cirrhosis. Cell Physiol Biochem 2016;40:1117-28
Yilmaz-Ozden T, Can A, Karatug A, Pala-Kara Z, Okyar A, Bolkent S. Carbon tetrachloride-induced kidney damage and protective effect of Amaranthus lividus L. in rats. Toxicol. Ind. Health 2014; 32(6): 1143–52. doi:10.1177/0748233714555390
Bellassoued K, Ben Hsouna A, Athmouni K.van Pelt J, Makni AF, Rebai T, Elfeki A. Protective effects of Mentha piperita L. leaf essential oil against CCl4 induced hepatic oxidative damage and renal failure in rats. Lipids Health Dis 2018;17: 9.
Cohen SM, Bevan C, Gollapudi B, Klaunig JE. Evaluation of the carcinogenicity of carbon tetrachloride. J Toxicol Environ Health, Part B 2023;26(6): 342–370.
Makni M, Chtourou Y, Garoui EM, Boudawara T, Fetoui H. Carbon tetrachloride-induced nephrotoxicity and DNA damage in rats: protective role of vanillin. Hum Exp Toxicol. 2012;(8):844-52.
Ali H, Kabir N, Muhammad A, Shah MR, Musharraf SG, Iqbal N, Nadeem S. Hautriwaic acid as one of the hepatoprotective constituent of Dodonaea viscosa. Phytomed.. 2014;21(2):131-40.
Rahman, S., Husen, A. Potential role of medicinal plants in the cure of liver and kidney diseases. In: Husen, A., Bachheti, R.K., Bachheti, A. (eds) Non-timber forest products Cham: Springer; 2021
Numbere AO, Camilo GR. Structural characteristics, above ground biomass and productivity of mangrove forest situated in areas with different levels of pollution in the Niger Delta, Nigeria. Afr J Ecol 2018;56(4):917-927
Uddin SJ, Grice D, Tiralongo E. Evaluation of cytotoxic activity of patriscabratine, tetracosane and various flavonoids isolated from the Bangladeshi medicinal plant Acrostichum aureum. Pharm Biol 2012;50 (10): 1276-1280
Zhong Y, Yu R, Chen Y, Zhou R, Liu Y.The complete chloroplast genomes of the mangrove fern Acrostichum aureum. Mitochondrial DNA Part B 2020, 5(3): 2258–2259.
Hossain H, Jahan IA, Nimmi I, Hossain A, Kawsar H. Anti-inflammatory activity of the ethanolic extract of Acrostichum aureum (Linn.) root. Bangladesh Pharm. J 2011;14: 2:107-109.
Akinwumi KA, Abam EO, Oloyede ST, Adeduro MN, Adeogun YA, Uwagboe JE. Acrostichium aureum Linn: traditional use, phytochemistry, and biological activity. Clin. Phytosci. 2022;8(1):1-18.
Badhsheeba MA, Vadivel V. Physicochemical and phytochemical contents of the leaves of Acrostichum aureum L. J Global Biosci. . 2020;9(4):7003-18.
Badhsheeda A, Vadivel, V. Evaluation of in vitro antioxidant activity of Acrostichum aureum Linn. Rachis. J Pharmacogn Phytochem 2018;7 (6): 1146-1151
Khan SA, Hossain MA, Panthi S, Asadujjaman M, Hossin A. Assessment of antioxidant and analgesic activity of Acrostichum aureum Linn.(Family-Pteridaceae). PharmacologyOnline 2013;1:166-71.
Roni M, Harilal CC. Biotoxicity of Acrostichum aureum L. synthesized zinc oxide nanoparticles against Aedes albopictus, and impact on predation efficiency of mosquito fish Gambusia affinis. Inorg. Chem. Commun. 2023; 156: 111224.
Ultari A, Handayani D,Eriadi A. A review : study of chemical content , bioactivity of mangrove fern plants (Acrostichum aureum L). EAS J Pharm Pharmacol 2021;3(1): 3–8.
Awang NA, Ali AM, Mat N. Alternative Medicines from edible bitter plants of Besut, Malaysia J. Agrobiotech. 9 (2), 2018;80 – 91
Nurhasnawati H, Sundu R, Sapri S, Supriningrum R, Kuspradini H, Arung ET. Antioxidant activity, total phenolic and flavonoid content of several indigenous species of q0ferns in East Kalimantan, Indonesia. Biodiversitas 2019;20(2):576–80.
Basyuni M, Putri LAP, Julayha Nurainun H, Oku H. Non-saponifiable lipid composition of four salt-secretor and non-secretor mangrove species from north Sumatra, Indonesia. Makara J Sci. 2012 2012;16 (2): 89-94
Uddin SJ, Jason TL, Beattie, K. D. Grice, D. and Tiralongo, E. Isolation of novel cytotoxic compounds from a Bangladeshi medicinal plant Acrostichum aureum. Planta Med. 2011;77: 19
Thomas AP, Peter, KJ, Chandramohanakumar N. A profiling of anti-tumour potential of sterols in the mangrove fern Acrostichum aureum. Int. J. Pharmacogn. Pharm. Res. 2016; 8 (11): 1828-1832.
Minh TT, Thu NH, Toan HK, Quang TT, Thanh NT, Huong TT, Van Sung T. Three new phenolic sulfates from Acrostichum aureum collected from coastal area of Thai Binh Province, Vietnam and their cytotoxic activity. Rec Nat Prod. 2022;16(1):66-73.
Wu X, Huang Q, Xu N, Cai J, Luo D, Zhang Q, Su Z, Gao C, Liu Y. Antioxidative and anti-inflammatory effects of water extract of Acrostichum aureum Linn. against ethanol-induced gastric ulcer in rats. eCAM 2018: 3585394.
Devi RK, Rajesh NV, Vasantha S, Geetha VS. Anti-parasiticaction of Actinopteris radiata, Acrostichum aureum and Hemionitis arifolia. Pteridological Res. 2015;4:1-9
Shamsuddin AA, Najiah M, Suvik A, Azariyah MN, Kamaruzzaman BY, Effendy AW, John B.A. Antibacterial properties of selected mangrove plants against Vibrio species and its cytotoxicity against Artemia salina. World Appl. Sci. J. 2013;25:333–340.
Uddin SJ. Guillon, P. Grice ID, Tiralongo E. In-vitro antiviral activity of a novel phthalic acid ester derivative isolated from the Bangladeshi mangrove fern Acrostichum aureum. J Antiviral Antiretroviral 2013;5:6 http://dx.doi.org/10.4172/jaa.1000078
Lai HY, Lim YY. Evaluation of antioxidant activities of the methanolic extracts of selected ferns in Malaysia. Int. J. Sci. Environ Sci. Dev2011;2: 442-447.
Akinwumi KA, Jubril AJ, Olaniyan OO, Umar YY. Ethanol extract of Nigella sativa has antioxidant and ameliorative effect against nickel chloride-induced hepato-renal injury in rats. Clin. Phytosci. 2020;6(1):1-12.
National Research Council. Guide for the care and use of laboratory animals, 8th ed., Washington USA: National Academic Press; 2011.
Percie du Sert, N, Hurst V, Ahluwalia A, Alam S, Avey MT, Baker,M, BrowneWJ, Clark A, Cuthill,IC, Dirnagl U, Emerson M, Garner P, Holgate ST, Howells DW, Karp 38.NA, Lazic SE, Lidster K, MacCallum CJ,Macleod M, Pearl EJ, Petersen OH,Rawle F, Reynolds P, Rooney K, Sena ES, Silberberg SD, Steckler T, Würbel H. The ARRIVE guidelines 2.0: updated guidelines for reporting animal research. PLoS Biol 2020;18:e3000410.
Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products, malondialdehyde and 4-hydroxynonenal. Method Enzymol 1990;186: 407-421.
Sun M, Zigma S. An improved spectrophotometric assay of superoxide dismutase based on epinephrine antioxidation. Anal Biochem 1978;90:81-89.
Habig, WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem1974;249(22):7130–7139.
Rotruck JT, Pope AL, Ganther HE. Prevention of oxidative damage to rat erythrocytes by dietary selenium. J Nutr 1972; 102(5):689-96.
Akinwumi KA, Olabode OO, Wakeel AA. The protective effect of methanol extract of Rauvolfia vomitoria against the clastogenicity and hepatotoxicity of sodium arsenite in mice. J. Can. Res. Updates. 2018;7(4):109-18
Orijemie EA. Exploitation of aquatic resources in Ahanve, Badagry, South-Western Nigeria. Internet Archaeol 37. 2014; http://dx.doi.org/10.11141/ia.37.8
Li R, Yang W, Yin Y, Ma X, Zhang P, Tao K. 4-OI attenuates carbon tetrachloride-induced hepatic injury via regulating oxidative stress and the inflammatory response. Front Pharmacol 2021;12:651444
Lee YS, Cho IJ, Kim JW, Lee MK, Ku SK, Choi JS, Lee HJ. Hepatoprotective effects of blue honeysuckle on CCl4-induced acute liver damaged mice. Food Sci Nutr. 2018 ;7(1):322-338
Zamzami MA, Baothman OAS, Samy F, Abo-Golayel MK. Amelioration of CCl4-induced hepatotoxicity in rabbits by Lepidium sativum Seeds. eCAM.2019:5947234
Yang C, Li L, Ma Z, Zhong Y, Pang W, Xiong M, Fang S and Li Y: Hepatoprotective effect of methyl ferulic acid against carbon tetrachloride-induced acute liver injury in rats. Exp Ther Med 2018;15: 2228-2238.
Shaban NZ, El-Kot SM, Awad OM,.Afaf MH, Ghada MF. The antioxidant and anti-inflammatory effects of Carica papaya Linn. seeds extract on CCl4-induced liver injury in male rats. BMC Complement Med Ther 2021;21: 302.
Ujowundu FN, Kalu JO, Ujowundu CO, Onyeocha IO, Onuoha CH, Ibeh RC, Obasi UK, Ntaji OE, Chigbu IN, Ezirim CY. Investigating the effect of flavonoid, saponin, alkaloids and tannins extracted from Combretum dolichopentalum Diels in CCl4-induced hepatotoxicity. Trop J Nat Prod Res 2022;6(8);1255-1261
Wang Z, Sun W, Sun X, Wang Y, Zhou M. Kaempferol ameliorates cisplatin-induced nephrotoxicity by modulating oxidative stress, inflammation and apoptosis via ERK and NF-κB pathways. AMB Expr 2020;10:58.
Song J, Kim Y, Lee J. Comparison of antioxidant and anti-inflammatory activity of quercetin, isoquercitrin and rutin against alcohol‐induced liver injury in HepG2 Cells. FASEB J.. 2018 32:670-0.
Youssef FS, Ashour ML, El-Beshbishy HA, Ahmed Hamza A, Singab ANB, Wink M. Pinoresinol-4-O-β-D-glucopyranoside: a lignan from prunes (Prunus domestica) attenuates oxidative stress, hyperglycaemia and hepatic toxicity in vitro and in vivo. J Pharm Pharmacol. 2020;72(12):1830-1839.
Huang S, Mo C, Zeng T, Lai Y, Zhou C, Xie S, Chen L, Wang Y, Chen Y, Huang S, Gao L. Lupeol ameliorates LPS/D-GalN induced acute hepatic damage by suppressing inflammation and oxidative stress through TGFβ1-Nrf2 signal pathway. Aging 2021;13(5): 6592.
Devaraj E, Roy A Veeraragavan GR Magesh A. β-Sitosterol attenuates carbon tetrachloride-induced oxidative stress and chronic liver injury in rats. Naunyn-Schmiedeberg's Arch Pharmacol 2020; 393(6):1-9.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Tropical Journal of Natural Product Research (TJNPR)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
