Fagonia longispina Batt. extracts: Antioxidant, Antibacterial, and Antiproliferative effects on Cancer Cells

Authors

  • Radja Djebbari Department of Organism Biology, Batna University, 2–53, Road to Constantine. Fésdis, Batna 05078, Algeria.
  • Somia Lassed Department of Microbiology and Biochemistry, Batna University, 2–53, Road to Constantine. Fésdis, Batna, 05078, Algeria.
  • Widad Ourzeddine Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.
  • Sana Aouachria Department of Microbiology and Biochemistry, Batna University, 2–53, Road to Constantine. Fésdis, Batna, 05078, Algeria.
  • Muhammed Altun Plant Research Laboratory, Department of Chemistry, Faculty of Science, Çankırı Karatekin University, Uluyazı Campus, 18100 Çankırı, Turkey
  • Ibrahim Demirtas Plant Research Laboratory, Department of Chemistry, Faculty of Science, Çankırı Karatekin University, Uluyazı Campus, 18100 Çankırı, Turkey.
  • Amina M. Lehneche Department of Natural Science, Training School of Teachers "Assia Djebar", Constantine University Town Ali Mendjli, Constantine, Algeria.
  • Scheherazed Dakhmouche-Djekrif Department of Natural Science, Training School of Teachers "Assia Djebar", Constantine University Town Ali Mendjli, Constantine, Algeria.
  • Mohammed Kebieche LMAGECA and BMBP research laboratories, Batna University 2–53, Road to Constantine. Fésdis, Batna 05078, Algeria
  • Fadila Benayache Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.
  • Samir Benayache Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.
  • Djamila Zama Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

DOI:

https://doi.org/10.26538/tjnpr/v8i6.6

Keywords:

antiproliferative activity, antibacterial activity, antioxidant activity, lipid peroxidation, phenolic compounds, Fagonia longispina

Abstract

Fagonia longispina (Zygophyllaceae family) has long been employed in traditional practices for combating cancer, with scientific research yielding insights into its biological impact. This study aimed to validate the potential of this plant by evaluating the antioxidant, antiproliferative, and antibacterial properties of the n-butanol, ethyl acetate, and chloroform extracts of F. longispina (BuE, EAE, and ChE, respectively). The most significant total phenolics (TPC) were recorded in EAE (377 ± 24.33 µg GAE/mg extract), whereas BuE showed the most important quantity of flavonoids (TFC) (87.14 ± 1.56 µg QE/mg extract). Outcomes indicated that EAE displayed the highest DPPH (2, 2-diphenyl-1-picrylhydrazyl) scavenging effect (IC50 = 37.82 ± 7.83 μg/mL) compared to BuE (IC50 = 59.49 ± 6.16 μg/mL, p = 0.0197) and ChE (IC50 =111.89 ± 6.10 μg/mL, p = 0.0002). Despite the increased activity of EAE, it remained lower than ascorbic acid (IC50 = 5.00 ± 0.1 µg/mL, p = 0.02). Moreover, EAE was the strongest at scavenging free radicals from FeSO4 (IC50 = 126.66 ± 5.76 µg/mL). All extracts except BuE showed antiproliferative efficacy against HeLa and HT-29 cells. Various bacterial strains underwent testing to demonstrate their antibacterial effectiveness, which was assessed by measuring inhibition zones. The results showed enjoyable antimicrobial activity against gram-positive bacteria. In conclusion, this study proves the application of F. longispina in traditional medicine. Furthermore, the results from the antioxidant tests confirmed that F. longispina had an antioxidant effect and may be used as a new natural antioxidant in therapy.  

Author Biographies

Radja Djebbari, Department of Organism Biology, Batna University, 2–53, Road to Constantine. Fésdis, Batna 05078, Algeria.

Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

Somia Lassed, Department of Microbiology and Biochemistry, Batna University, 2–53, Road to Constantine. Fésdis, Batna, 05078, Algeria.

Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

Djamila Zama, Research Unit of Natural Resources Valuation, Bioactive Molecules, Physicochemical and Biological Analysis (VARENBIOMOL), University Constantine 1, Ain El Bey Road, 25000 Constantine, Algeria.

 

 

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Published

2024-06-29

How to Cite

Djebbari, R., Lassed, S., Ourzeddine, W., Aouachria, S., Altun, M., Demirtas, I., … Zama, D. (2024). Fagonia longispina Batt. extracts: Antioxidant, Antibacterial, and Antiproliferative effects on Cancer Cells. Tropical Journal of Natural Product Research (TJNPR), 8(6), 7374–7381. https://doi.org/10.26538/tjnpr/v8i6.6