Isolation and Characterization of Flavonol glycoside as Antioxidant and Antidiabetic Compounds from Ebony (DiospyroscelebicaBakh.)Leaf Ethanol Extract

Authors

  • Lisya Apriandini Department of Pharmacy, Majoring Herbal Medicine, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
  • Muhammad Hanafi Department of Pharmacy, Majoring Herbal Medicine, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
  • Ratna Djamil Department of Pharmacy, Majoring Herbal Medicine, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
  • Yesi Desmiaty Department of Pharmacy, Majoring Herbal Medicine, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia
  • Puspa D.N Lotulung Research Center for Pharmaceutical Ingredients and Traditional Medicine, Indonesian National Research and Innovation Agency (BRIN), Banten, Indonesia
  • Nina Artanti Research Centre for Chemistry, Indonesian National Research and Innovation Agency (BRIN), Banten, Indonesia

DOI:

https://doi.org/10.26538/tjnpr/v8i6.5

Keywords:

Kaempferol-3-O-β-glucoside, α-glucosidase, Antidiabetic, Antioxidant, Diospyros celebica

Abstract

Ebony (Diospyroscelebica.Bakh) is an Indonesian medicinal plant used as antidiabetic herbs. A previous study found that the ethanol extract of ebony leaf has strong antioxidant and α-glucosidase inhibitory activity. The aim of this study was to isolate and characterize antioxidant and antidiabetic compounds from the leaves of Ebony. Powdered ebony leaves were extracted with 96% ethanol, and then fractionated with ethyl acetate. The ethyl acetate fraction was purified by column chromatography which led to the isolation of compound Fr.36-13a.The structure of compoundFr.36-13awas elucidated through a combination of 1H-NMR, 13C-NMR, and 2D-NMR spectroscopy. The antioxidant activity of compound Fr.36-13awas determined by DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging assay. The antidiabetic activity of the compound was assessed in vitro by the α-glucosidase inhibitory assay. Compound Fr.36-13a was identified as kaempferol-3-O-β-glucoside through the 1D and 2D NMR data, and by comparison of the spectroscopic data with literature values. Compound Fr.36-13a (Kaempferol-3-O-β-glucoside) showed strong antioxidant and antidiabetic activities by scavenging DPPH radical and inhibiting α-glucosidase enzyme. Kaempferol-3-O-β-glucoside could serve as a potential therapeutic agents for the treatment of diabetes mellitus. 

Author Biography

Muhammad Hanafi, Department of Pharmacy, Majoring Herbal Medicine, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia

Research Centre for Chemistry, Indonesian National Research and Innovation Agency (BRIN), Banten, Indonesia

References

Ihwan, Anisa S, Khumaidi A, Khaerati K. Activity of Ebony Leaf Extract (Diospyros celebica Bakh) on Healing Diabetic Wounds of White Rats Induced by Alloxan. Med Sains: J Ilmiah Kefarmasian. 2023;8(2):539-548.

Chen J, Ni C, Lou J, Peng W. Molecules and functions of rosewood: Diospyros celebica. Arab JChem. 2018;11(6):756-762.

Kartini K, Khumaidi A, Khaerati K, Ihwan I. Ethanolic Extract of Eboni Leaf (Diospyros celebica Bakh) Decrease Blood Glucose Level in Alloxan-Induced Male Rats. J Vet. 2018;19(3):329-334.

Megawati, Anam S, Pitopang R. Ethnobotanical Study of Medicinal Plants in the Kaili Ija Tribe Community in Bora Village, Sigi Biromaru District, Sigi Regency, Central Sulawesi. Biocelebes J. 2016;10(1):76-90.

Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: A comprehensive review. Chinese Med. 2018;13(1):20.

Apriandini L, Hanafi M, Djamil R, Desmiaty Y, Narrij PD, Artanti N. Antioxidant and Antidiabetic Compounds from Ebony Leaf Extract (Diospyros celebica Bakh.) and Their Correlation with Total Phenolic and Flavonoid Compounds in Various Solvent Extraction. J Nat Prod Degener Dis. 2023;1(1):8-18.

Ramadhan R, Rosyidah N, Firdaus YFH, Kurnia IT, Lestari DA, Phontree K, Phuwapraisirisan R, Riyadi L, Rahman A, Abdulgani N, Maretna SA, Fadhila KN. Phytochemical analysis in conjunction with in vitro α-glucosidase inhibitory and antioxidant activities of three Diospyros species from East Kalimantan, Indonesia. Biodivers J. 2023;24(9):4783-4790.

Ajaegbu EE, Uzochukwu IC, Okoye FBC. Antioxidant and Anti-inflammatory Activities of Extract and Fractions of Spondias mombin Leaf and Isolation of its Active Principles. Trop J Nat Prod Res. 2022;6(1):80-86.

Purba YR and Sembiring HB. Isolation and Identification of Phenolic Compounds from Ebony Plant Leaves (Diospyros celebica Bakh.). J Chem Nat Resour. 2022;04(02):146-152.

Djamil R, Pratami DK, Putri FAYU, Octavia TB. Determination of Quality Parameters and Test Antioxidant Activities of 70 % Ethanol Extract of Seroja Leaves (Nelumbo nuciera Gartn .). Int J Appl Pharm Sci Res. 2021;13(2):30-33.

Djamil R, Winarti W, Zaidan S, Abdillah S. Pharmacognosy & Natural Products Antidiabetic Activity of Flavonoid from Binahong Leaves (Anredera cordifolia) Extract in Alloxan-Induced Mice. J Pharmacogn Nat Prod. 2017;3(2):2-5.

Taiwo FO, Oyedeji O, Osundahunsi MT. Antimicrobial and Antioxidant Properties of kaempferol-3-O-glucoside and 1-(4-Hydroxyphenyl)-3-phenylpropan-1-one Isolated from the Leaves of Annona muricata (Linn.). J Pharm Res Int. 2019; 26(3):1-13.

Ekasari CP, Widyarti S, Sumitro SB. The Analysis of Antioxidant Activity and Capacity of Boiled and Infused Indonesian Herbals. Trop J Nat Prod Res. 2023;7(1):2145-2151.

Sarian MN, Ahmed QU, Mat So’Ad SZ, Alhassan AM, Murugesu S, Perumal V, Syed Mohamad SNA, Khatib A, Latip J. Antioxidant and antidiabetic effects of flavonoids: A structure-activity relationship based study. Biomed Res Int. 2017;2017:8386065.

Aba PE and Asuzu IU. Mechanisms of actions of some bioactive anti-diabetic principles from phytochemicals of medicinal plants : A review. Indian J Nat Prod Resour. 2018; 9(2):85-96.

Nguyen T, Lien NLP, Nguyet NTM. Determination of Phenolics , Antioxidant Activity and Antidiabetic Potential of Sphagneticola calendulacea (L.) Pruski Leaves Grown in Vietnam. Trop J Nat Prod Res. 2023;7(5):2992-2995.

He C, Liu X, Jiang Z, Geng S, Ma H, Liu B. Interaction Mechanism of Flavonoids and α-Glucosidase: Experimental and Molecular Modelling Studies. Foods. 2019;8(9):335.

Cahyana Y and Adiyanti T. Review: Flavonoids as antidiabetic agents. Indonesian J Chem. 2021;21(2):512-526.

Downloads

Published

2024-06-29

How to Cite

Apriandini, L., Hanafi, M., Djamil, R., Desmiaty, Y., Lotulung, P. D., & Artanti, N. (2024). Isolation and Characterization of Flavonol glycoside as Antioxidant and Antidiabetic Compounds from Ebony (DiospyroscelebicaBakh.)Leaf Ethanol Extract. Tropical Journal of Natural Product Research (TJNPR), 8(6), 7368–7373. https://doi.org/10.26538/tjnpr/v8i6.5