Assessment of Biosurfactant Production and Petroleum Hydrocarbons Biodegradation Capability of Actinomycetes Isolated from Soils

Bolanle A. Akinsanola; Olusoji O. Adebisi; Omorefosa O. Osemwegie

doi:10.26538/tjnpr/v8i5.39

Authors

Bolanle A. Akinsanola Department of Food Science and Microbiology, Landmark University, Omu-Aran, Kwara State, Nigeria.
Olusoji O. Adebisi Department of Microbiology, University of Ilorin, Ilorin, Nigeria
Omorefosa O. Osemwegie Department of Food Science and Microbiology, Landmark University, Omu-Aran, Kwara State, Nigeria.

DOI:

https://doi.org/10.26538/tjnpr/v8i5.39

Keywords:

Actinomycetes, biosurfactants, petroleum hydrocarbons, bioremediation

Abstract

Production of biosurfactants and emulsifiers by bacteria has been suggested to enhance the biodegradability of hydrocarbons (HCs) in complex matrices like crude oil and its refined products. Recent studies have focused on the capability of actinobacteria to biodegrade HCs because of their abundance in soils and high tolerance to recalcitrant chemicals. Here, actinomycetes isolated from pesticide-impacted agricultural soils were assessed for their biosurfactant production and complex HC biodegradation capabilities. Five actinomycetes (Lentzea albidocapillata, Actinomyces slackii, Actinomyces liubingyangii Rhodococcus erythropolis and Trueperella bernardiae) identified based on morphological, physiological, and 16S rRNA gene sequencing were assessed for biosurfactant production using four tests. The biodegradation capability was assayed in aviation fuel, petrol, kerosene, diesel and crude oil using the standard CO₂respirometry for 30 days. All isolates exhibited high emulsifying activity (35.71 - 65.12%) in all HCs but their ability to adhere to hydrophobic surfaces varied greatly (28.77-70.11%) with L. albidocapillata demonstrating the most significant hydrophobicity. With the exception of T. bernardiae, all actinomycetes' whole-cell suspensions and cell-free supernatants generated sizable biosurfactants that removed diesel film from water more effectively than crude oil. Biosurfactants produced by A. liubingyangii caused the greatest displacement of the oils. The complex HCs were mineralized to different extents, with the highest extent of mineralization by L. albidocapillata in petrol (53.40mgCO₂/mL). The findings imply that the ability of these actinomycetes to adhere to hydrophobic matrices and produce biosurfactants with strong emulsifying activity makes them good candidates for bioremediation of complex HCs like crude oil and its refined products.

References

Olajuyigbe FM, Ehiosun KI. Assessment of crude oil degradation efficiency of newly isolated actinobacteria reveals untapped bioremediation potentials. Bioremediat J. 2016;20(2):133–43.

Ndimele PE, Saba AO, Ojo DO, Ndimele CC, Anetekhai MA, Erondu ES. Remediation of Crude Oil Spillage. Polit Ecol Oil Gas Act Niger Aquat Ecosyst. 2018;369–84.

Varjani SJ. Microbial degradation of petroleum hydrocarbons. Bioresour Technol [Internet]. 2017;223:277–86. Available from:

http://dx.doi.org/10.1016/j.biortech.2016.10.037

Cristóvão RO, Pinto VMS, Martins RJE, Loureiro JM, Boaventura RAR. Assessing the influence of oil and grease and salt content on fish canning wastewater biodegradation through respirometric tests. J Clean Prod. 2016;127:343–51.

Mary Kensa V. Bioremediation - An overview. J Ind Pollut Control. 2011;27(2):161–8.

Kuppusamy S, Thavamani P, Singh S. Polycyclic aromatic hydrocarbons ( PAHs ) degradation potential , surfactant production , metal resistance and enzymatic activity of two novel cellulose-degrading bacteria isolated from koala faeces. Environ Earth Sci. 2017;

Tadayon Tajabadi M, Sabernejad A, Khalili Najafabadi M. Biosurfactant-producing Microorganisms: Potential for Bioremediation of Organic and Inorganic Pollutants. Res Biotechnol Environ Sci. 2023;2(2):18–23.

Prudence SMM, Addington E, Castaño-Espriu L, Mark DR, Pintor-Escobar L, Russell AH, McLean TC. Advances in actinomycete research: An actinobase review of 2019. Vol. 166, Microbiology (United Kingdom). 2020. p. 683–94.

Quatrini P, Scaglione G, De Pasquale C, Riela S, Puglia AM. Isolation of Gram-positive n-alkane degraders from a hydrocarbon- contaminated Mediterranean shoreline. J Appl Microbiol. 2008;104(1):251–9.

Shamikh YI, El Shamy AA, Gaber Y, Abdelmohsen UR, Madkour HA, Horn H, Hassan HM, Elmaidomy AH, Alkhalifah DHM, Hozzein, WN. Actinomycetes from the red sea sponge coscinoderma mathewsi: Isolation, diversity, and potential for bioactive compounds discovery. Microorganisms. 2020;8(5):783.

Das N, Chandran P. Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview. Biotechnol Res Int. 2011;2011:1–13.

Van Beilen JB, Funhoff EG. Alkane hydroxylases involved in microbial alkane degradation. Vol. 74, Applied Microbiology and Biotechnology. 2007; 13–21.

Fan Y, Wang Y, Qian PY, Gu JD. Optimization of phthalic acid batch biodegradation and the use of modified Richards model for modelling degradation. Int Biodeterior Biodegrad. 2004;53(1):57–63.

Patowary K, Patowary R, Kalita MC, Deka S. Characterization of biosurfactant produced during degradation of hydrocarbons using crude oil as sole source of carbon. Front Microbiol. 2017;8:1–14.

Petrikov K, Delegan Y, Surin A, Ponamoreva O, Puntus I, Filonov A, et al. Glycolipids of Pseudomonas and Rhodococcus oil-degrading bacteria used in bioremediation preparations: Formation and structure. Process Biochem [Internet]. 2013;48(5–6):931–5. Available from: http://dx.doi.org/10.1016/j.procbio.2013.04.008

Rosenberg M, Rosenberg E. Role of adherence in growth of Acinetobacter calcoaceticus RAG-1 on hexadecane. J Bacteriol. 1981;148(1):51–7.

Abdulrasheed M, Zakaria NN, Roslee AFA, Shukor MY, Zulkharnain A, Napis S, et al. Biodegradation of diesel oil by cold-adapted bacterial strains of Arthrobacter spp. From Antarctica. Antarct Sci. 2020;32(5):341–53.

Maneerat S, Phetrong K. Isolation of biosurfactant-producing marine bacteria and characteristics of selected biosurfactant. Songklanakarin J Sci Technol. 2007;29(3):781–91.

Masaaki Morikawa , Yoshihiko Hirata TI. Izracun prehodne vrtilne hitrosti radialnih drsnih lezajev. Stroj Vestnik/Journal Mech Eng. 1989;35(1-3l):37–41.

Johnson FA, Dosunmu A, Aleruchi OB. Environmental Impact Assessment of Current Synthetic Drilling Fluids inOffshore Oil Fields Using Modified CO2 Evolution Respirometry Technique. Soc Pet Eng - SPE Niger Annu Int Conf Exhib NAIC 2023. 2023;

Weidner S, Kittelmann M, Goeke K, Ghisalba O, Zähner H. 3’-Demethoxy-3’-hydroxystaurosporine-O-methyltransferase from Streptomyces longisporoflavus catalyzing the last step in the biosynthesis of staurosporine. J Antibiot (Tokyo). 1998;51(7):679–82.

Jai Godheja SKS. Growth Potential Assessment of Actinomycetes Isolated from Petroleum Contaminated Soil. J Bioremediation Biodegrad. 2014;05(07):2

Prez-Armendriz B, Mauricio-Gutirrez A, Jimnez Salgado T, Tapia-Hernndez A, Santiesteban Lpez A. Emulsification of Hydrocarbons Using Biosurfactant Producing Strains Isolated from Contaminated Soil in Puebla, Mexico. In: Biodegradation - Engineering and Technology [Internet]. InTech; 2013. Available from: http://www.intechopen.com/books/biodegradation-engineering-and-technology/emulsification-of-hydrocarbons-using-biosurfactant-producing-strains-isolated-from-contaminated-soil