Hepatoprotective Effects of Honey Against Tobacco Smoking Toxicity in Wistar Rats


  • Alia Khwaldeh Faculty of Allied Medical Sciences, Department of Medical Laboratory Sciences, Jadara University, P.O.Box 733, Irbid 21110, Jordan
  • Ziad Shraideh Department of Biological Sciences, School of Science, the University of Jordan, Amman, Jordan
  • Darwish Badran Department of Anatomy and Histology, School of Medicine, the University of Jordan, Amman, Jordan
  • Aiman Shoiab Faculty of Pharmacy, Department of Pharmacy, Jadara University, P.O.Box 733, Irbid 21110, Jordan
  • Ali Ata Alsarhan Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Jordan
  • Saad Al-Fawaeir Faculty of Allied Medical Sciences, Department of Medical Laboratory Sciences, Jadara University, P.O.Box 733, Irbid 21110, Jordan
  • Ahmed Alzbeede Department of Medical Laboratory Science, College of Science, Komar University of Science and Technology, Sulaymaniyah, Iraq
  • Abdulrahim Aljamal Department of Biological Sciences, Al Al-Bayt University, Al-Mafraq, Jordan




Honey, Hepatoprotective, Cigarette, Waterpipe, Smoking


Honey has been used as a nutritional diet and traditional medicine due to its antimicrobial and antioxidant properties. This study aims to evaluate the hepatoprotective potential of honey against the hepatoxic effect of cigarette and waterpipe tobacco smoking in rats. Honey was purchased from the local market. A daily dose of 1.2 g/kg of honey was administered orally to 48 male Wistar rats, and a digital smoking device was used to expose rats to waterpipe and cigarette tobacco smoke before dissection. The enzymatic activities of the liver using a serum sample were quantified, and liver tissue was isolated and prepared for light microscopy for histopathological examination. The hepatoprotective effect of honey was studied in rats in combination with cigarette and waterpipe smoking. The current investigation demonstrates that honey exhibits a substantial protective effect on liver tissue in rats exposed to cigarette and waterpipe smoking. Based on the study findings, honey exhibits a hepatoprotective effect.


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How to Cite

Khwaldeh, A., Shraideh, Z., Badran, D., Shoiab, A., Alsarhan, A. A., Al-Fawaeir, S., … Aljamal, A. (2024). Hepatoprotective Effects of Honey Against Tobacco Smoking Toxicity in Wistar Rats. Tropical Journal of Natural Product Research (TJNPR), 8(5), 7220–7224. https://doi.org/10.26538/tjnpr/v8i5.25