Biotypology of Culicidian Species in the Region of Fez, Central Morocco Using the Statistical Analytical Methods


  • Touria Filali Mouatassem Laboratory of Biotechnology, Conservation, Development of Natural Resources, Faculty of Sciences, Sidi Mohamed-ben-Abdellah University, Dhar-El-Mahraz, 30000 Fez, Morocco.
  • Chafika Faraj Laboratory of Medical Entomology, National Institute of Hygiene, Rabat, BP 769, Agdal, Rabat, Morocco
  • Raja Guemmouh Laboratory of Biotechnology, Conservation, Development of Natural Resources, Faculty of Sciences, Sidi Mohamed-ben-Abdellah University, Dhar-El-Mahraz, 30000 Fez, Morocco.
  • Mouhcine Fadil Physicochemical Laboratory of Inorganic and Organic Materials, Material Science Center (MSC), Ecole Normale Supérieure, Mohammed V University, Rabat, BP: 5118 NU, Morocco
  • Noureddine Rais Laboratory of Computer Science, Modelling and Systems, Sidi Mohamed Ben Abdellah University, Faculty of Sciences Dhar Mahraz, Fez 30000, Morocco
  • Hicham El Asmi El Asmi Geosciences, Environment, and Associated Resources Laboratory, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Dhar El Mahraz, 30000 Fez, Morocco
  • Fouad El-Akhal Higher Institute of Nursing Professions and Health Techniques of Tetouan, Regional Health Directorate, Mohammed V Hospital, Ministry of Health and Social Protection, Al Hoceima, 32000, Morocco
  • Youness El Fattouhi Laboratory of Biotechnology, Conservation, Development of Natural Resources, Faculty of Sciences, Sidi Mohamed-ben-Abdellah University, Dhar-El-Mahraz, 30000 Fez, Morocco.
  • Najoua Darkaoui Laboratory of Biotechnology, Conservation, Development of Natural Resources, Faculty of Sciences, Sidi Mohamed-ben-Abdellah University, Dhar-El-Mahraz, 30000 Fez, Morocco.
  • Asmae Baghouz Laboratory of Biotechnology, Conservation, Development of Natural Resources, Faculty of Sciences, Sidi Mohamed-ben-Abdellah University, Dhar-El-Mahraz, 30000 Fez, Morocco.
  • Abdelhakim El Ouali Lalami Laboratory of Biotechnology, Conservation, Development of Natural Resources, Faculty of Sciences, Sidi Mohamed-ben-Abdellah University, Dhar-El-Mahraz, 30000 Fez, Morocco



Biotypology, Central Morocco, Culicidian species, Disease vectors, PCA, MCA


Controlling mosquitoes responsible for discomfort and serious contagious diseases, such as malaria, requires a better understanding of the vector-environment relationship. Therefore, the present study examined the biotypology of Culicidian species in the Fez Region, Central Morocco, using statistical analytical methods. Entomological investigations and measurements of the physicochemical parameters of the water breeding sites were carried out simultaneously from November 2015 to December 2016 in the region of Fez, Central Morocco. Multivariate analytical methods, including principal component analysis (PCA) and multiple correspondence analysis (MCA), were used to investigate the correlation between environmental factors and the bio-ecological characteristics of each species. The identification of 1,531 Culicidae larvae collected from the different breeding sites revealed the presence of 11 species belonging to five genera (AnophelesCulexCulisetaAedes, and Uranotaenea). The larvae of Anopheles maculipennis (the major malaria vector in Morocco) and the larvae of A. sergentii (the secondary vector), as well as Culex pipiens larvae, were collected among the species at the level of the prospected breeding sites. It was observed that the Shanon-Wiener diversity index (H’) values were higher in areas with a high population of species compared to those with a low population of species. The multivariate analytical methods revealed many groups of species depending on many parameters. This research represents an original and rigorous approach to the vector-environment relationship in the Fez Region of Morocco. It has important implications for the control of mosquito vectors and the prevention of vector-borne diseases, thereby contributing to public health in the region.

Author Biography

Abdelhakim El Ouali Lalami, Laboratory of Biotechnology, Conservation, Development of Natural Resources, Faculty of Sciences, Sidi Mohamed-ben-Abdellah University, Dhar-El-Mahraz, 30000 Fez, Morocco

Higher Institute of Nursing Professions and Health Techniques of Fez, Regional Health Directorate, El-Ghassani Hospital, Ministry of Health an



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How to Cite

Mouatassem, T. F., Faraj, C., Guemmouh, R., Fadil, M., Rais, N., El Asmi, H. E. A., … Lalami, A. E. O. (2024). Biotypology of Culicidian Species in the Region of Fez, Central Morocco Using the Statistical Analytical Methods. Tropical Journal of Natural Product Research (TJNPR), 8(5), 7172–7180.

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