Mice as Small Animal Model for <i>Human Norovirus</i> Infection

Authors

  • Warda E. Rusdi Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Soetjipto Department of Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Marie I. Lusida Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Zayyin Dinana Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
  • Aussie T. Maharani Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia
  • Irmawan Farindra Department of Anatomy and Histology, Faculty of Medicine, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia
  • Nailul Huda Faculty of Medicine, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia
  • Radia R. Maharani Faculty of Medicine, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia
  • Ayu A. A. Hamida Faculty of Medicine, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia

DOI:

https://doi.org/10.26538/tjnpr/v8i5.6%20

Keywords:

Diarrhea, Mice, Animal Model, Norovirus

Abstract

Despite being discovered in 1972, the development of vaccines to prevent Human Norovirus (HuNoV) infection in humans remains limited. This limitation is partly due to inadequate knowledge about Norovirus infection in non-human hosts and its pathogenic process. This research was performed to assess the efficacy of HuNoV infection in mice as an animal model by using both different infection routes and virus titers. In this research, 20 BALB/c Mus musculus were used as the test animals which were randomly divided into 4 groups. Intraperitoneal and oral infection methods were employed using different viral titers. The mice were euthanized on the fifth day to obtain fecal samples from the colon organ. Afterwards, Quantitative Polymerase Chain Reaction (qPCR) was administered to evaluate these samples. Most of the mice did not show symptom, except one mouse that experienced diarrhea. The HuNoV virus replication in the mice was measured using qPCR analysis. Three of the twenty mice showed elevated virus titers after being infected with HuNoV. In conclusion, BALB/c strain mice remained resistant to HuNoV infection when exposed to the virus through the oral route. Conversely, the use of high viral titers for intraperitoneal infection indicated the presence of viral replication.

Author Biography

Warda E. Rusdi, Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Department of Public Health, Faculty of Medicine, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia

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Published

2024-05-30

How to Cite

Rusdi, W. E., Soetjipto, Lusida, M. I., Dinana, Z., Maharani, A. T., Farindra, I., … Hamida, A. A. A. (2024). Mice as Small Animal Model for <i>Human Norovirus</i> Infection. Tropical Journal of Natural Product Research (TJNPR), 8(5), 7089–7092. https://doi.org/10.26538/tjnpr/v8i5.6