ERRATUM: Effect of <i>Cinnamomum burmannii</i> Bark Oil on Bacterial Count, Nuclear Factor of Kappa Beta (NF-Kβ/p65), Interleukin-6, and Chest Radiography in <i>Rattus norvegicus</i> with Pneumonia

Authors

  • Ana Majdawati Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
  • Reviono Reviono Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
  • Hartono Hartono Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
  • Nestri Handayani Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Surakarta, Indonesia
  • Soetrisno Soetrisno Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

DOI:

https://doi.org/10.26538/tjnpr/v8i1.18

Keywords:

wistar rats, pneumonia, Cinnamomum burmannii bark oil, anti-inflammatory, antibacterial

Abstract

Pneumonia is the second most common lung infection caused by Multidrug-Resistant Klebsiella pneumoniae. This study aimed to analyze the effect of Cinnamomum burmannii Bark oil (CbBO) as an antibacterial (decreasing bacterial counts) and anti-inflammatory agent through the inhibition of Immunoreactive Score Nuclear Factor Kappa Beta (IRSNF-Kβ/p65) and Interleukin 6 (IL-6), and chest radiography/CXR analysis of male Wistar rats' model Klebsiella pneumoniae (Kp). The experimental animals were divided into seven groups consisting of normal control group (C), negative control (NC, Kp 100 µL), Treatment 1 (T1:Kp+ Carboxymethylcellulose sodium 3 mL), Treatment 2 (T2:Kp + Levofloxacin 13.5 mg/200 gram BW of rats), Treatment 3-5 (T3, T4, T5: Kp+CbBO 0.36; 0.72; and 1.44 µL/200 gram BW of rats). The treatment was given for the first, second, and third days po st-inoculation. All parameters on day 4 were examined. The bacterial count, IRS NF-Kβ/p65, and IL-6 are parametric data evaluated using Kruskall Wallis and post hoc tests. The CXR, a non-parametric data, was analysed with a chi-square test. The results showed that T3 and T4 decreased in all parameters. There was a significant difference test between NC compared T4 on the bacterial count (p-value=0.033), NC compared T3 (p-value=0.05), and T5 (p-value=0.037) on the IRS NF- Kβ/p65, and T1 compared T4 (p-value=0.045) on CXR. This study concluded that CbBO doses of 0.36 and 0.72 µL/200 gram BW of rats reduce all parameters.

Author Biographies

Ana Majdawati, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Radiology, Faculty of Medicine and Health Sciences,  Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia

Reviono Reviono, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia@umy.ac.id

Hartono Hartono, Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

Department of Physiology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia

References

Perhimpunan Dokter Paru Indonesia. Pneumonia : Pedoman Diagnosis dan Tata Laksana Medis. Ikat Dr Indones. 2020;(19):19–22.

Baptista, C.T., Cerveira, M.M., Barboza, V., Maria, E., Ferrer, K., Miller, R.G., Souza, T. De, Zank, P.D., Blanke, A.D.O., Klein, V.P., Rosado, R.P., Silveira, R.M., Melo, L.G. De, Martin, C., Pereira, P. De, Lopes, Q., Giongo, J.L., Vaucher, R.D.A. A Systematic Review of Essential Oils’ Antimicrobial and Antibiofilm Activity against Klebsiella pneumoniae. Curr Res Complement Altern Med. 2022;6(3).

Juan CH, Fang SY, Chou CH, Tsai TY, Lin YT. Clinical characteristics of patients with pneumonia caused by Klebsiella pneumoniae in Taiwan and prevalence of antimicrobial-resistant and hypervirulent strains: a retrospective study. Antimicrob Resist Infect Control. 2020;9(1):1–8.

Labbaci FZ, Belkhodja H, Elkadi FZ, Megharbi A, Belhouala K. Tropical Journal of Natural Product Research HPLC-MS Analysis and evaluation of Antioxidant and Anti-Inflammatory Potential of Cinnamomum cassia Extract. 2023;7(August):3637–42.

Ács K, Balázs VL, Kocsis B, Bencsik T, Böszörményi A, Horváth G. Antibacterial activity evaluation of selected essential oils in liquid and vapor phase on respiratory tract pathogens. BMC Complement Altern Med. 2018;18(1):1–9.

Yanakiev S. Effects of Cinnamon (Cinnamomum spp.) in Dentistry: A Review. Molecules. 2020;25(18).

Wicaksana PF, Wulandari DP, Kusuma A, Sastrowijoto S. The difference in biofilms formations on duration less than 90 d and more than 90 d of tracheotomy cannula usage. J Med Sci (Berkala Ilmu Kedokteran). 2023;55(1).

Yousef SA Al. The use of cinnamon oil as antibacterial agent to eliminate some antibiotic-resistant bacteria isolated from water sources. Acta Fytotech Zootech. 2021;24(1):1–8.

Al-Mariri A, Safi M. In vitro antibacterial activity of several plant extracts and oils against some gram-negative bacteria. Iran J Med Sci. 2014;39(1):36–43.

Das M, Mandal S, Mallick B, Hazra J. Ethnobotany, Phytochemical and Pharmacological Aspects of Cinnamomum Zeylanicum Blume. Int Res J Pharm. 2016;4(4):58–63.

Yang, S.K., Yusoff, K., Ajat, M., Thomas, W., Abushelaibi, A., Akseer, R., Lim, S.H.E., Lai, K.S. Disruption of KPC-producing Klebsiella pneumoniae membrane via induction of oxidative stress by cinnamon bark (Cinnamomum verum J. Presl) essential oil. PLoS One. 2019;14(4):1–20.

Prajapati JA, Humbal BR, Sadariya KA, Shailesh K. Determination of in-vivo anti-inflammatory potential of Cinnamomum zeylanicum oil in female wistar rats. Pharma Innov J 2019; 8(7) 544-547 ISSN. 2019;8(7):544–7.

Sharifi-Rad, J., Dey, A., Koirala, N., Shaheen, S., El Omari, N., Salehi, B., Goloshvili, T., Cirone Silva, N.C., Bouyahya, A., Vitalini, S., Varoni, E.M., Martorell, M., Abdolshahi, A., Docea, A.O., Iriti, M., Calina, D., Les, F., López, V., Caruntu, C. Cinnamomum Species: Bridging Phytochemistry Knowledge, Pharmacological Properties and Toxicological Safety for Health Benefits. Front Pharmacol. 2021;12(May):1–27.

Budiastuti, Nurcholida RD, Primaharinastiti R, Sukardiman. Anti-inflammatory activity of cinnamon bark oil (cinnamomum burmannii (Nees & T. Nees) blume from lombok timur indonesia. Pharmacogn J. 2021;13(4):1005–13.

Yudhawati R, Yuniawati E. Correlation of serum interleukin-6 level and pneumonia severity index score in patient with community-acquired pneumonia. J Adv Pharm Educ Res. 2021;11(3):58–62.

Zhao C, Cao Y, Zhang Z, Nie D, Li Y. Cinnamon and eucalyptus oils suppress the inflammation induced by lipopolysaccharide in vivo. Molecules. 2021;26(23).

Arisma A, Sukmanadi M, Plumeriastuti H, Helmi Effendi M, . B, Marty Yanestria S. The effectiveness of Cinnamomum (Cinnamomum burmannii) Essential Oil on the Reduction of Inflamation Levels in White Rat Livers (Rattus norvegicus) Induced by Streptozotocin. Ecol Environ Conserv. 2022;28(01s):11–11.

Moradi S, Fazlali A, Hamedi H. Microwave-assisted hydro-distillation of essential oil from rosemary: Comparison with traditional distillation. Avicenna J Med Biotechnol. 2018;10(1):22–8.

Professor A, Lecturer S. Comparative Study on Steam Distillation and Hydro-Distillation Methods for Agarwood Oil Extraction. Int J Appl Eng Res [Internet]. 2018;13(8):6253–6. Available from: http://www.ripublication.com

Izzah N. Minyak Atsiri: Proses Produksi melalui Metode Klasik. Warung Sains Teknologi [Internet]. 2022; Available from: https://warstek.com/minyak-atsiri-produks/

Rebecca Jaspan, RD C. The Health Benefits of Cinnamon May reduce risks of heart disease, lower blood sugar, and fight infections [Internet]. 2022. Available from: https://www.verywellfit.com/cinnamon-for-health-89013

Bacharach DRL and AL. Evaluation of drug activities, pharmacometrics, ed. 1st ed. 1964.

Bergamini, G., Perico, M.E., Palma, S. Di, Sabatini, D., Andreetta, F., Defazio, R., Felici, A., Ferrari, L. Mouse pneumonia model by Acinetobacter baumannii multidrug resistant strains: Comparison between intranasal inoculation, intratracheal instillation and oropharyngeal aspiration techniques. PLoS One [Internet]. 2021;16(12 December):1–18. Available from: http://dx.doi.org/10.1371/journal.pone.0260627

Wisnuwardhani PH. Biosafety laboratory practices : Pedoman Umum Keselamatan Kerja Pada Laboratorium Biosafety Level 3. J BioTrends [Internet]. 2018;9(2):1–10. Available from: https://terbitan.biotek.lipi.go.id/index.php/biotrends/article/download/242/204

Rijal N. Spread Plate Technique: Principle, Procedure, Results [Internet]. General Microbiology. 2022. Available from: https://microbeonline.com/spread-plate-technique/

Mustika Anggiane Putri AKMDAKMPAYI. Rat Anasthesia Protocol-DR.MUSTIKA- Medical Faculty of Trisakti University _(2)_. [cited 2021 May 24]; Available from: http://www.karyailmiah.trisakti.ac.id/uploads/kilmiah/dosen/PROTOKOL_ANESTESI_TIKUS-DR.MUSTIKA-Medical Faculty FK TRISAKTI(2)_.pdf

Cicero L, Fazzotta S, Palumbo VD, Cassata G, Lo Monte AI. Anesthesia protocols in laboratory animals used for scientific purposes. Acta Biomed. 2018;89(3):337–42.

Do Nascimento, L.D., de Moraes, A.A.B., da Costa, K.S., Galúcio, J.M.P., Taube, P.S., Costa, C.M.L., Cruz, J.N., Andrade, E.H. de A., de Faria, L.J.G. Bioactive natural compounds and antioxidant activity of essential oils from spice plants: New findings and potential applications. Biomolecules. 2020;10(7):1–37.

Liu, D., Chen, Z., Yuan, Y., Jing, H., Zou, J., Zhang, X., Zeng, X., Zhang, W., Zou, Q., Zhang, J. Innate Immune Effectors Play Essential Roles in Acute Respiratory Infection Caused by Klebsiella pneumoniae. J Immunol Res. 2020;2020.

Zhang, B., Swamy, S., Balijepalli, S., Panicker, S., Mooliyil, J., Sherman, M.A., Parkkinen, J., Raghavendran, K., Suresh, M. V. Direct pulmonary delivery of solubilized curcumin reduces severity of lethal pneumonia. FASEB J [Internet]. 2019;33(12):13294–309. Available from: http://dx.doi.org/10.1096/fj.201901047RR

Stein AM, Peletier LA. Predicting the Onset of Nonlinear Pharmacokinetics. CPT Pharmacometrics Syst Pharmacol. 2018;7(10):670–7.

Zhang, B., Swamy, S., Balijepalli, S., Panicker, S., Mooliyil, J., Sherman, M.A., Parkkinen, J., Raghavendran, K., Suresh, M. V. Direct pulmonary delivery of solubilized curcumin reduces severity of lethal pneumonia. FASEB J [Internet]. 2019;33(12):13294–309. Available from: http://dx.doi.org/10.1096/fj.201901047RR

Rosario menendez, Jose Miguel Sahoquillo-arce, Raquel martinez, Eva polverino, catia Cilloniz, Juan Gines Cordoba, Beatriz Montull and AT. Cytokine Activation Patterns and Biomarkers Are Influenced by Microorganisms in Community-Acquired Pneumonia. Chest [Internet]. 2012;141(6):1537–45. Available from: www.chestpubs.org

Chakotiya A, Narula A, Sharma R. Efficacy of methanol extract of Zingiber officinale rhizome against acute pneumonia caused by Pseudomonas aeruginosa. J Lung Heal Dis. 2018;2(1):1–8.

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Published

2024-05-01

How to Cite

Majdawati, A., Reviono, R., Hartono, H., Handayani, N., & Soetrisno, S. (2024). ERRATUM: Effect of <i>Cinnamomum burmannii</i> Bark Oil on Bacterial Count, Nuclear Factor of Kappa Beta (NF-Kβ/p65), Interleukin-6, and Chest Radiography in <i>Rattus norvegicus</i> with Pneumonia. Tropical Journal of Natural Product Research (TJNPR), 8(4), 5832–5839. https://doi.org/10.26538/tjnpr/v8i1.18

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