Protective Role of Plant Growth Promoting Rhizobacteria Inoculation in the Development of Drought Tolerance in Shallot: Effects on Hydroxygen Peroxide Production, Lipid Peroxidation, and Secondary Metabolite Production
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Abstract
Shallot contains secondary metabolites and antioxidants that can be used as raw materials for traditional medicine. However, shallot has the disadvantage of being intolerant to drought. Drought can affect the quality of the compounds in shallot at a certain level. Therefore, this study investigates the protective role of Plant Growth Promoting Rhizobacteria(PGPR) inoculation in conferring drought tolerance to shallot during different growth stages.Two factors and three replications were considered in this study. The first factor was the timing of drought stress, comprising four treatments (vegetative phase, bulb initiation, bulb development, and maturation), with one treatment as a control without drought stress. The second factor was the type of bacteria, consisting of two treatments (Bacillus subtilisPb03and Pseudomonas fluorescensPb04), with one treatment as a control without PGPR inoculation. The research results indicate that oxidative stress triggered by drought stress is evidenced by an increase in hydrogen peroxide production, lipid peroxidation, and secondary metabolites at almost all stages of growth. Treatment Bacillus subtilis Pb03 inoculation was more effective thanPseudomonas fluorescens Pb04 in mitigating drought stress in shallots. Bacillus subtilis Pb03 inoculation inhibited oxidative stress by enhancing the activity of antioxidant enzymes. Additionally, this application suppressed the production of secondary metabolites, thereby maintaining osmotic balance in the plants.
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References
Nhung TT, Quoc LP. Counteracting Paracetamol-Induced Hepatotoxicity with Black Shallot Extract: An Animal Model Investigation. Trop J. of Nat Prod Res. 2024; 8(1): 5875–5880. Doi: 10.26538/tjnpr/v8i1.24
Khagendra BP, Yoshihisa S, Sameh KA, Tetsuya S, Mohamed S. Impacts of climate change on drought and its consequences on the agricultural crop under worst-case scenario over the Godavari River Basin, India. Clim Serv. 2023; 32: 100415. Doi: 10.1016/j.cliser.2023.100415
Boy R, Indradewa D, Putra ETS, Kurniasih B. Drought-induced production of reactive oxygen species and antioxidants activity of four local upland rice cultivars in Central Sulawesi, Indonesia. Biodiv. 2020; 21(6): 2555-2565. Doi: 10.13057/biodiv/d210628
Hasanuzzaman M, Bhuyan M, Zulfiqar F, Raza A, Mohsin S, Mahmud J, Fujita M, Fotopoulos V. Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxid. 2020; 9(8): 681. Doi: 10.3390/antiox9080681
Deepika S, Bharti S, Satish K, Vikas K, Shweta S, Shivam S. Stress induced production of plant secondary metabolites in vegetables: Functional approach for designing next generation super foods. Plant Physiol and Biochem. 2022; 192: 252-272. Doi: 10.1016/j.plaphy.2022.09.034
Farooq M, Hussain M, Ul-Allah S, Siddique KH. Physiological and agronomic approaches for improving water-use efficiency in crop plants. Agr Water Mgt. 2019; 219: 95-108. Doi: 10.1016/j.agwat.2019.04.010
Rabisa Z, Muhammad SN, Muhammad JS, Sughra H, Asma I. Plant survival under drought stress: Implications, adaptive responses, and integrated rhizosphere management strategy for stress mitigation. Microbiol Res. 2021; 242: 126626. Doi: 10.1016/j.micres.2020.126626
Jamwal K, Bhattacharya S, Puri S. Plant growth regulator mediated consequences of secondary metabolites in medicinal plants. J. of Appl Res on Med and Aromat Plant. 2018; 9:26-38. Doi: 10.1016/j.jarmap.2017.12.003
Kaushal M, Wani SP. Rhizobacterial-plant interactions: Strategies ensuring plant growth promotion under drought and salinity stress. Agr, Ecosyst and Environ. 2016; 231: 68-78. Doi: 10.1016/j.agee.2016.06.031
Vurukonda SS, Krishna P, Sandhya V, Manjari S, Ali SZ. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiol Res. 2016; 184: 13-24. Doi: 10.1016/j.micres.2015.12.003
Jing D, Binghua L, Hailin M, Fangchun L, Xinghong L, Liying R. Effects of Inoculation with Different Plant Growth-Promoting Rhizobacteria on the Eco-Physiological and Stomatal Characteristics of Walnut Seedlings under Drought Stress. Agron. 2023; 13(6): 1486. Doi: 10.3390/agronomy13061486
Chieb M, Gachomo E. The role of plant growth promoting rhizobacteria in plant drought stress responses. BMC Plant Biol. 2023; 23(1): 407. Doi: 10.1186/s12870-023-04403-8
Uzma M, Iqbal A, Hasnain S. Drought tolerance induction and growth promotion by indole acetic acid producing Pseudomonas aeruginosa in Vigna radiata. PLoS ONE. 2022; 17(2): e0262932. Doi: 10.1371/journal.pone.0262932
Fairoj S, Islam M, Zaman E, Momtaz M, Hossain M, Jahan N, Shams SNU, Urmi T, Rasel M. Salicylic Acid Improves Agro-Morphology, Yield and Ion Accumulation of Two Wheat (Triticum aestivum L.) Genotypes by Ameliorating the Impact of Salt Stress. Agron. 2023; 13(25): 13010025. Doi: 10.3390/agronomy13010025
Wintermans JFG, De Mots A. Spectrophotometric characteristics of Chlorophylls a and b and their pheophytins in etanol. Biochimia Biophysica Acta. 1965; 109: 448-453.
Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amer J. of Enol and Viticult. 1965; 16(3): 144-158.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colometric methods. J. of Food and Drug Anal. 2022; 10(3): 178-182. Doi: 10.38212/2224-6614.2748
Bates L, Waldren R, Teare I. Rapid determination of free proline for water-stress studies. Plant Soil.1973; 39: 205-207. Doi: 10.1007/BF00018060
Patricia P, Celina MH, Aparecida SS, Vera SNP, Maria TB. Evaluation of allicin stability in processed garlic of different cultivars. Food Sci and Technol. 2014; 34(3): 623-628. Doi: 10.1590/1678-457x.6397
Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Sci. 2000; 151: 59-66. Doi: 10.1016/S0168-9452(99)00197-1
Madhava RK, Sresty T. Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Sci. 2000; 157(1): 113-128. Doi: 10.1016/s0168-9452(00)00273-9
Islam M, Jahan K, Sen A, Urmi T, Haque M, Ali H, Siddiqui M, Murata Y. Exogenous Application of Calcium Ameliorates Salinity Stress Tolerance of Tomato (Solanum lycopersicum L.). Antioxid (Basel). 2023; 12(3): 558. Doi: 10.3390/antiox12030558
Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981; 22: 867-880. Doi: 10.1093/oxfordjournals.pcp.a076232
Dhindsa RS, Matowe W. Drought Tolerance in Two Mosses: Correlated with Enzymatic Defence Against Lipid Peroxidation. J. of Exp Bot. 1981; 32(1): 79-91. Doi: 10.1093/jxb/32.1.79
Margalida RO, Mateu FP, Josefina B, Jaume F. Adjustments in photosynthesis and leaf water relations are related to changes in cell wall composition in Hordeum vulgare and Triticum aestivum subjected to water deficit stress. Plant Sci. 2021; 311: 111015. Doi: 10.1016/j.plantsci.2021.111015
Zhenqi L, Junliang F, Zhenlin L, Zhentao B, Haidong W, Minghui C, Fucang Z, Zhijun L. Chapter Three - Response network and regulatory measures of plant-soil-rhizosphere environment to drought stress. Advances in Agron. 2023; 180:93-196. Doi: 10.1016/bs.agron.2023.03.002
Jha Y, Subramanian R, PGPR regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity. Physiol Mol Biol Plants. 2014; 20: 201–207. Doi: 10.1007/s12298-014-0224-8
Sarma B, Kashtoh H, Lama Tamang T, Bhattacharyya PN, Mohanta YK, Baek K-H. Abiotic Stress in Rice: Visiting the Physiological Response and Its Tolerance Mechanisms. Plants. 2023; 12(23):3948. Doi: 10.3390/plants12233948
Seleiman M, Al-Suhaibani N, Ali N, Akmal M, Alotaibi M, Refay Y, Dindaroglu T, Abdul-Wajid H, Battaglia M, Drought Stress Impacts on Plants and Different Approaches to Alleviate Its Adverse Effects. Plants (Basel). 2021; 10(2): 259 Doi: 10.3390/plants10020259
Chou S, Chen B, Chen J, Wang M, Wang S, Croft H, Shi Q, Estimation of leaf photosynthetic capacity from the photochemical reflectance index and leaf pigments. Ecol Indic. 2020; 110: 105867 Doi: 10.1016/j.ecolind.2019.105867
Hafiz GMDA, Abdus SK, Ming JL, Sultan HK, Muhammad K. Early selection of bread wheat genotypes using morphological and photosynthetic attributes conferring drought tolerance. J. of Integr Agric. 2019; 18(11): 2483-2491 Doi: 10.1016/S2095-3119(18)62083-0
Nurcholis W, Ma’rifah K, Artika MI, Aisyah SI, Priosoeryanto BP. Optimization of Total Flavonoid Content from Cardamom Fruits Using a Simplex-Centroid Design, Along with the Evaluation of the Antioxidant Properties. Trop J of Nat Prod Res. 2021; 5(8): 1382–1388 Doi: 10.26538/tjnpr/v5i8.10
Mohammad AH, Anamul HmD, David BJ, Masayuki F. Chapter 16 - Proline Protects Plants Against Abiotic Oxidative Stress: Biochemical and Molecular Mechanisms. Oxid Damage to Plants; 2019: 477-522p Doi: 10.1016/B978-0-12-799963-0.00016-2
Behvar A, Raheleh K, Behnam S. Plant growth promoting rhizobacteria (PGPR) confer drought resistance and stimulate biosynthesis of secondary metabolites in pennyroyal (Mentha pulegium L.) under water shortage condition. Sci Hortic. 2020; 263: 109132 Doi: 10.1016/j.scienta.2019.109132
Du Y, Zhao Q, Chen L, Yao X, Xie F. Effect of Drought Stress at Reproductive Stages on Growth and Nitrogen Metabolism in Soybean. Agron. 2020; 10(2):302. Doi: 10.3390/agronomy10020302
Muhammad AF, Adnan KN, Javaid A, Saifullah, Muhammad F, Zahra S, Naser K, Zed R. Acquiring control: The evolution of ROS-Induced oxidative stress and redox signaling pathways in plant stress responses. Plant Physiol. Biochem. 2019; 141: 353-369 Doi: 10.1016/j.plaphy.2019.04.039
Nxele X, Klein BNA. Drought and salinity stress alters ROS accumulation, water retention, and osmolyte content in sorghum plants. S. Afr. J. Bot. 2017; 108: 261-266 Doi: 10.1016/j.sajb.2016.11.003
Gharibi S, Tabatabaei B, Saeidi G, Effect of Drought Stress on Total Phenolic, Lipid Peroxidation, and Antioxidant Activity of Achillea Species. Appl Biochem Biotechnol. 2016; 178: 796–809 Doi: 10.1007/s12010-015-1909-3
Yousefvand P, Sohrabi Y, Heidari G, Weisany W, Mastinu A. Salicylic Acid Stimulates Defense Systems in Allium hirtifolium Grown under Water Deficit Stress. Molecules. 2022; 27(10): 27103083 Doi: 10.3390/molecules27103083
Zhou R, Kong L, Yu X. Oxidative damage and antioxidant mechanism in tomatoes responding to drought and heat stress. Acta Physiol Plant. 2019; 41(20): 1-11 Doi: 10.1007/s11738-019-2805-1
Shuxue H, Hongxia C, Hubing W, Xiaoyan P, The physiological responses of tomato to water stress and re-water in different growth periods. Sci Hortic.2019; 249:143-154 Doi: 10.1016/j.scienta.2019.01.045
Anam K, Munandar R, Wulandari ON, Lestari AB, Farada RE, Hudiyanti D, Aminin AL. Chemical Composition, Antioxidant Activities, and Total Phenolic Content of Combination of Mangosteen (Garcinia mangostana L.) Peel-Kodavan (Centella asiatica L. Urban) Fractions. Trop J. of Nat Prod Res. 2023; 7(1): 2222–2228 Doi: 10.26538/tjnpr/v7i1.20