<I>In vitro</I> Anti-diabetic and Anti-oxidative Evaluation of Hydro-methanol Bark Extract of <I>Bauhinia acuminata</I> (L.)


  • Sajani Singharoy Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, West Midnapore, West Bengal- 721102, India.
  • Dibya Pal Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, West Midnapore, West Bengal- 721102, India.
  • Shibani Das Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, West Midnapore, West Bengal- 721102, India.
  • Debidas Ghosh Molecular Medicine, Nutrigenomics and Public Health Research Laboratory, Department of Bio-Medical Laboratory Science and Management, Vidyasagar University, West Midnapore, West Bengal- 721102, India.




Liquid chromatography-mass spectrophotometry, Anti-oxidative, Anti-diabetic, Hydro-methanol extract, Bauhinia acuminata (L)


Diabetes mellitus (DM) is a multifactorial disease of significant public health concern globally. This study aimed to assess the in-vitro effect of hydro-methanol (3:2) Bauhinia acuminata stem bark extract on diabetes and diabetes-linked oxidative stress to develop an herbal-based product against diabetic complications. Qualitative analysis, TLC, and LC-MS were performed to determine the phytomolecule(s) in the extract. Diabetes was induced in rats by a single intramuscular injection of streptozotocin. The liver, kidney, small intestine, heart, and skeletal muscle were dissected from the control and diabetic groups on the 29th day of streptozotocin injection and placed immediately into the in-vitro media and dosed with the extract except for the control and untreated diabetic groups. Carbohydrate metabolic enzymes, anti-oxidative markers, and general toxicity sensors were evaluated. Dosing of extract at 10, 20, and 40 mg/10 mL of in-vitro media reduced the activity of glucose-6-phosphatase significantly (p<0.05), but hexokinase activity in liver, skeletal, and cardiac muscle were unchanged compared with the untreated diabetic group. Inhibition in enteric alpha-glucosidase activity was noted. The activities of antioxidant enzymes and thiobarbituric acid reactive substances level were recovered significantly (p<0.05) in the hepatic and renal tissues after extract treatment compared to the untreated diabetic group. Glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities were reduced in the tissues of treated groups compared to the untreated diabetic group. This study showed that at 1mg/ml, the extract exhibited significant effects on the metabolic function of diabetic rat tissues with an increase in endogenous antioxidant levels.


Singer ME, Dorrance KA, Oxenreiter MM, Yan KR, Close KL. The type 2 diabetes' modern preventable pandemic' and replicable lessons from the COVID-19 crisis. Prev Med Rep. 2022; 25:1-6.

Chaudhary N, Tyagi N. Diabetes mellitus: An overview. Int J. Res Dev Pharm L Sci. 2018; 7: 3030-3033.

Sun H, Saeedi P, Karuranga S, Pinkepank M, Ogurtsova K, Duncan BB, Stein C, Basit A, Chan JCN, Mbanya JC, Pavkov ME, Ramachandaran A, Wild SH, James S, Herman

WH, Zhang P, Bommer C, Kuo S, Boyko EJ, Magliano DJ. IDF Diabetes Atlas: global, regional, and country-level diabetes prevalence estimates for 2021 and projections for 2045. Diabetes Res Clin Pract. 2022; 183:1-13.

Qaid MM, Abdelrahman MM. Role of insulin and other related hormones in energy metabolism- A review. Cogent Food Agric. 2016; 2:1-19.

Buchberger B, Huppertz H, Krabbe L, Lux B, Mattivi JT, Siafarikas A. Symptoms of depression and anxiety in youth with type 1 diabetes: A systematic review and meta-analysis. PNEC. 2016; 70:70-84.

Khotimah H, Wulandari AM, Ainiyati SO, Wiyasa WA. A systematic review on the hypoglycemic potentials of mahogany (Swietenia spp.) seeds as antioxidants in the management of gestational diabetes mellitus. Trop J. Nat Prod Res. 2024; 8:6472-6479.

Yaribeygi H, Atkin SL, Sahebkar A. A review of the molecular mechanisms of hyperglycemia-induced free radical generation leading to oxidative stress. J. Cell Physiol 2019; 234:1300-1312.

Chaudhury A, Duvoor C, Reddy Dendi VS, Kraleti S, Chada A, Ravilla R, Marco A, Shekhawat NS, Montales MT, Kuriakose K, Sasapu A, Beebe A, Patil N, Musham CK, Lohani GP, Mirza W. Clinical review of anti-diabetic drugs: implications for type 2 diabetes mellitus management. Front Endocrinol (Lausanne). 2017; 8:1-12.

Sebastian D, Sophy R. Bauhinia acuminata Linn: A brief review of its phytochemistry and pharmacology. Asian J. Pharm Pharmacol. 2020; 6:164-170.

Roy MN, Naz T, Khan A, Ali H. Anti-diabetic potential of methanolic extract of leave and bark of Bangladeshi medicinal plant Bauhinia acuminata L on mice. J. Diabetes Metab. 2017; 8:1-3.

Okoro U, Kenechukwu FC, Ogbonna JDN, Omeje EO, Attama AA. Formulation development and optimization of herbo synthetic lipospheres based on solidified reverse micellar solutions for therapeutic management of diabetes mellitus. Trop J. Nat Prod Res. 2024; 8:6651-6662.

Sarkar R, Ghosh P, Tripathy A, Ghosh D. Correction of diabetes-induced testicular dysfunction by a hydro-methanol (60:40) extract of Curcuma amada rhizomes: A dose-dependent study. J. Food Biochem. 2019; 43: e12829.

Amarat W. Artemisia judaica attenuates hyperglycaemia-mediated oxidative stress and cardiac injury in streptozotocin-induced diabetic rats. Trop J. Nat Prod Res. 2020; 4:722-727.

Stubbs M, Robinson SP, Rodrigues LM, Parkins CS, Collingridge DR, Griffiths JR. The effects of host carbogen (95% oxygen/5% carbon dioxide) breathing on methanolic characteristics of Morris hepatoma 9618a. Br J. Cancer. 1998; 78:1449-1456.

Maiti R, De D, Ghosh D. Anti-diabetic effect of n-hexane fraction of hydro-methanolic extract of Tamarindus indica linn seed in streptozotocin-induced diabetic rat: A correlative approach with in vivo and in vitro antioxidant activities. Int J. Pharm Sci Res. 2018; 9:1821-1830.

Nithiya T, Udayakumar R. Antihyperglycemic effect of an important phytocompound-phloretin on streptozotocin induced diabetes: an experimental study. J. Adv Med Pharm. 2016; 7:1-10.

Thilagam E, Parimaladevi B, Kumarappan C, Mandal SC. α-glucosidase and α-amylase inhibitory activity of Senna surattensis. J. Acupunct Meridian Stud. 2013; 6:24-30.

Trawczynska I. New method of determining kinetic parameters for decomposition of hydrogen peroxide by catalase. Catalysts. 2020; 10:1-12.

Zhang QA, Wang X, Song Y, Fan XH, García Martín JF. Optimisation of pyrogallol autoxidation conditions and its application in evaluation of superoxide anion radical scavenging capacity for four antioxidants. J. AOAC Int. 2016; 99:504-511.

Olorunfemi RM, Oluwakemi VA, Stephen AA, Adeyemi OA, Adeniyi SO. In vitro anti-oxidant and anti-diabetic potentials of the seed, bark and whole pod of okra (Abelmoschus esculentus(L.) moench): A comparative study. Trop J. Nat Prod Res. 2024; 8:6451-6456.

Ganesan J, Kavitha AV. Recovery of phosphatase and transaminase activity of mercury intoxicated Mus musculus (Linn.) liver tissue by Tribulus terrestris (Linn.) (Zygophyllaceae) extract. Trop Biomed. 2006; 23: 45-51.

Okokon JE, Udobang JA, Bassey AI, Edem UA, Agu EC. Hepatoprotective and nephroprotective activities of husk extract of Zea mays against paracetamol-induced liver and kidney injuries in rats. Trop J. Nat Prod Res. 2020; 4:67-76.

Fadhillah F, Yohandini H, Widjajanti H. Chemical compound isolated from antioxidant active extract of endophytic fungus Cladosporium tenuissimum in Swietenia mahagoni leaf stalks. Biodiversitas. 2019; 20:2645-2650.

Ara F, Tripathy A, Ghosh D. Dose dependent anti-diabetic activity of hydro-methanolic extract (3:2) of flower of M. balbisiana in streptozotocin induced diabetic male rat. Int J. Pharm Sci Res. 2019; 10:1000-1010.

Das P, Mitra D, Jana K, Ghosh D. In vitro study on spermicidal action of hydro-methanol extract of Tinospora cordifolia (wild) stem in rat and human sperm: a comparative analysis. Reprod Sci. 2023; 30:3480-3494.

Sokal RR, Rohle FJ. Introduction to analysis of variance. In: Sokal RR, Rohl FJ (Eds.). Biometry. New York: WH Freeman and Company; 1997. 179–206 p.

Bhatia A, Singh B, Arora R, Arora S. In vitro evaluation of the α-glucosidase inhibitory potential of methanolic extracts of traditionally used anti-diabetic plants. BMC Complement Altern Med. 2019; 19:1-9.

Assefa ST, Yang EY, Chae SY, Song M, Lee J, Cho MC, Jang S. Alpha glucosidase inhibitory activities of plants with focus on common vegetables. Plants. 2019; 9:1-17.

Charkoudian LK, Farrell BP, Khosla C. Natural product inhibitors of glucose-6-phosphate translocase. Med Chem Comm. 2012; 3:926-931.

Mandarino LJ, Printz RL, Cusi KA, Kinchington P, Doherty RM, Osawa H, Sewell C, Consoli A, Granner DK, DeFronzo RA. Regulation of hexokinase II and glycogen synthase mRNA, protein, and activity in human muscle. Am J. Physiol. 1995; 269:701-708.

Newsholme EA, Rolleston FS, Taylor K. Factors affecting the glucose 6-phosphate inhibition of hexokinase from cerebral cortex tissue of the guinea pig. Biochem J. 1968; 106:193-201.

Gonzalez‐Mujica F, Motta N, Estrada O, Perdomo E, Méndez J, Hasegawa M. Inhibition of hepatic neoglucogenesis and glucose‐6‐phosphatase by quercetin 3‐O‐α (2 ″‐galloyl) rhamnoside isolated from Bauhinia megalandra leaves. Phytother Res. 2005; 19:624-627.

Gonzalez‐Mujica F, Motta N, Becerra A. Inhibition of hepatic neoglucogenesis and glucose‐6‐phosphatase by aqueous extract of Bauhinia megalandra leaves. Phytother Res.1998; 12:291-293.

Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, Feng Y. The role of oxidative stress and antioxidants in liver diseases. Int J. Mol Sci. 2015; 16:1-38.

Kaur B, Henry J. Micronutrient status in type 2 diabetes: a review. Adv Food Nutr Res. 2014; 71:55-100.

Rana S, Dixit S, Mittal A. In silico target identification and validation for antioxidant and anti-inflammatory activity of selective phytochemicals. Braz Arch Biol Technol. 2019; 62:1-10.

Kakkar R, Mantha SV, Radhi J, Prasad K, Kalra J. Increased oxidative stress in rat liver and pancreas during progression of streptozotocin-induced diabetes. Clin Sci (Lond). 1998; 94:623-632.

De Leon JA, Borges CR. Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay. J. Vis Exp. 2020; 12:1-22.

Borra SK, Mahendra J, Gurumurthy P, Iqbal SS, Mahendra L. Effect of curcumin against oxidation of biomolecules by hydroxyl radicals. J. Clin Diagn Res. 2014; 8:1-5.

Honma K, Kamikubo M, Mochizuki K, Goda T. Insulin-induced inhibition of gluconeogenesis genes, including glutamic pyruvic transaminase 2, is associated with reduced histone acetylation in a human liver cell line. Metab Clin Exp. 2017; 71:118-124.

Perera S, Lohsoonthorn V, Jiamjarasrangsi W, Lertmaharit S, Williams MA. Association between elevated liver enzymes and metabolic syndrome among Thai adults. Diabetes Metab Syndr. 2008; 2:171-178.

Anusha CS, Sini H, Prakashkumar B, Nevin KG. Mechanism of protection of rat hepatocytes from acetaminophen-induced cellular damage by ethanol extract of Aerva lanata. Interdiscip Toxicol. 2019; 12: 169-179.

McGill MR. The past and present of serum aminotransferases and the future of liver injury biomarkers. EXCLI J. 2016; 15:817-828.

Mayengbam S, Raposo S, Aliani M, House JD. A vitamin B-6 antagonist from flaxseed perturbs amino acid metabolism in moderately vitamin B6 deficient male rats. J. Nutr. 2016; 146:14-20.

Salahudeen MS, Nishtala PS. An overview of pharmacodynamic modelling, ligand-binding approach and its application in clinical practice. Saudi Pharm J. 2017; 25:165-175.

Witte de WEA, Danhof M, van der Graaf PH, de Lange ECM. The implications of target saturation for the use of drug-target residence time. Nat Rev Drug Discov. 2018; 18:82-84.

Neubig RR, Spedding M, Kenakin T, Christopoulos A. International Union of Pharmacology Committee on receptor nomenclature and drug classification. XXXVIII. Update on terms and symbols in quantitative pharmacology. Pharmacol Rev. 2003; 55:597-606.




How to Cite

Singharoy, S., Pal, D., Das, S., & Ghosh, D. (2024). <I>In vitro</I> Anti-diabetic and Anti-oxidative Evaluation of Hydro-methanol Bark Extract of <I>Bauhinia acuminata</I> (L.). Tropical Journal of Natural Product Research (TJNPR), 8(4), 6932–6939. https://doi.org/10.26538/tjnpr/v8i4.26