Cytotoxic and Molecular Docking Potential of β-Sitosterol Isolated from Lantana camara Leaves against Breast (T47D) and Cervical Cancer (HeLa) Cell Lines
Article Sidebar
Main Article Content
Abstract
Lantana camara is a plant used in traditional medicine to treat various diseases.The cytotoxic potential of Lantana camara leaves has been reported for extracts and isolated pure compounds. So this study aims to test the cytotoxic potential ofβ-sitosterol isolated from the leaves ofLantana camarausingin silico (Molecular docking) as well asin-vitro MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against T47D and HeLa cancer cells. In this study, the isolation ofβ-sitosterol from the ethyl acetate extract of Lantana camara leaves was carried out by gravity column chromatography and purification by recrystallisation. The isolated compound was obtained as a white amorphous solid with a melting point of 136-137 °C. The structure of the isolated compound was determined by extensive IR,1H-NMR, and13C-NMR spectra data analysis and with comparison to literature data. The molecular docking results forβ-sitosterol showed good docking score values of -8.11 kcal/mol against Bcl- 2 and -7.276 kcal/mol against HPV16 E7, as well as a Root Mean Score Deviation (RMSD) value ≤ 2 compared to the standard anticancer drug doxorubicin, -9.230 kcal/mol against Bcl-2 and -8.288 kcal/mol (HPV16 E7), respectively. The results of the potential cytotoxic test ofβ-sitosterol using the MTT method showed its high cytotoxic properties against T47D (breast cancer cell line) and HeLa (cervical cancer cell line) with IC50 values of 9.98 µg/mL and 10.31 µg/mL,respectively.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Kalita S, Kumar G, Karthik L, Rao KVB. A review on medicinal properties of Lantana camara linn. Res J Pharm Technol. 2012;5(6):711–715.
Ediruslan, Manjang Y, Suryati, Aziz H. Structure Elucidation of Brine Shrimp Toxic Compound from Lantana camara L. Leaves. J Chem Pharm Res. 2015;7(12):250–255.
Suyati, Malasari Y, Efdi M, Mardiah E. A Cytotoxic Compound from n-Hexane Fraction of Lantana camara Linn Leaves. Molekul. 2019;14(1):31–36.
Suryati, Mardiah E, Efdi M, Mz K, Sari YM. Bioactivity of compounds isolated from the leaves of the Lantana camara Linn plant. J Chinese Pharm Sci. 2019;28(5):360–368.
Suryati, Aziz ED, Efdi M, Wahyuni FS, Hefni D. Analysis of The Essential Oil from Lantana camara Leaves and Its Cytotoxic Potential Against T-47D Breast Cancer Cells. J Ris Kim. 2021;12(1):1–9.
Suryati, Hefni D, Wahyuni FS, Dachriyanus. The cytotoxicity study of Lantana camara Linn essential oil on HeLa cancer cells line. Pharmacogn J. 2021;13(6):1498–1501.
Suryati, Santoni A, Arifin B, Ferdinal N, Salim E, Amelia A, Zein L, Mairanti S, Lestasri I. Analysis of Chemical Content, Cytotoxic and Anti-Bacterial Activity Essential Oil of Lantana Camara Linn Leaves from Various Regions. Molekul. 2022;17(2):156–164.
Suryati, Santoni A, Kartika MZ, Aziz H. Antioxidant activity and total phenolic content of ethyl acetate extract and fractions of Lantana camara L.leaf. Der Pharma Chem. 2016;8(8):92–96.
Septiani G, Susanti S, Sucitra F. Effect of Different Extraction Method on Total Flavonoid Contents of Sansevieria trifasciata P. Leaves Extract. Galen J Pharm. 2021;7(2):143–150.
Ayaz M, Sadiq A, Wadood A, Junaid M, Ullah F. Cytotoxicity and molecular docking studies on phytosterols isolated from Polygonum hydropiper L. Steroids. 2019;141(2019):30–35.
Nishitha G, Latha GA, Tejaswini L, Mounica A, Rekha K, Reddy NK, Jyothirmayi B, Kesana N, Unissa R. In vitro cytotoxic activity of ethyl acetate fraction of Hibiscus vitifolius flowers against hela cell line. Trop J Nat Prod Res. 2018;2(3):122–125.
Omoruyi SI, Enogieru AB, Ekpo OE. Preliminary Cytotoxic Activity of Sutherlandia frutescens and Carpobrotus edulis on Malignant glioblastoma Cells Sylvester. Trop J Nat Prod Res. 2019;3(5):175–179.
Adeyemi MM, Habila JD, Enemakwu TA, Okeniyi SO, Salihu L. Antimalarial Activity of Leaf Extract , Fractions and Isolation of Sterol from Alstonia boonei. Trop J Nat Prod Res. 2019;3(7):221–224.
Chaturvedula VSP, Prakash I. Isolation of Stigmasterol and beta-Sitosterol from the dichloromethane extract of Rubus suavissimus. Int Curr Pharm J. 2012;1(9):239–242.
Marisa D, Hayatie L, Juliati S, Suhartono E, Komari N. Molecular docking of phytosterols in Stenochlaena palustris as anti-breast cancer. Acta Biochim Indonesia. 2021;4(2):1–7.
Isman F, Pratama PR, Fadlan A. Molecular Docking of Michelia alba Leaves Active Compounds Against Human Epidermal Growth Factor Receptor 2 (HER-2). Al-Kimia. 2022;10(2):116–125.
Thuy BTP, My TTA, Hai NT, Hieu LT, Hoa TT, Loan HTP, Triet NT, Anh TTV, Quy PT, Tat PV, Quang NVH, Duong P, Trung NT, Tung VT, Huynh LK, Nhung NTA. Investigation into SARS-CoV ‑ 2 Resistance of Compounds in Garlic Essential Oil. ACS Omega. 2020;5(6):8312–8320.
Ramirez D, Caballero J. Is It Reliable to Take the Molecular Docking Top Scoring Position as the Best Solution without Considering Available Structural Data. Molecules. 2018;23(1038):1–17.
Walker JM. Mammalian Cell Viability. Martin J. Stoddart, editor. Switzerland: Humana Press; 2011. 103 p.
Baek J, Roh HS, Choi CI, Baek KH, Kim KH. Raphanus sativus sprout causes selective cytotoxic effect on p53-deficient human lung cancer cells in vitro. Nat Prod Commun. 2017;12(2):237–240.
Sajjadi SE, Ghanadian M, Haghighi M, Mouhebat L. Cytotoxic effect of Cousinia verbascifolia Bunge against OVCAR-3 and HT-29 cancer cells. J HerbMed Pharmacol. 2015;4(1):15–19.
Anwar MA, Tabassam S, Gulfraz M, Ahmad MS, Raja GK, Arshad M. Isolation of Oxyberberine and β -Sitosterol from Berberis lycium Royle Root Bark Extract and In Vitro Cytotoxicity against Liver and Lung Cancer Cell Lines. Hindawi Evidence-Based Complement Altern Med. 2020;2020:1–9.
Katja DG, Harneti D, Mayanti T, Maharani R, Shiono Y, Supratman U. Cytotoxic Steroids From The Stembak of Chisocheton celebicus KOORD. J Kim Val. 2019;5(2):143–148.
Silva ICV da, de Oliveira PF, Barbosa GM, Wessjohann LA, Cardozo-Filho L, Holandino C, Muzitano MF, Leal IVR. Passiflora mucronata leaves extracts obtained from different methodologies: A phytochemical study based on cytotoxic and apoptosis activities of triterpenes and phytosterols constituents. Brazilian J Pharm Sci. 2020;56:1–16.
Rekha K, Pandey R, Jain N, Supriya B, Babu KS, Rao JM. Chemical Constituents from Macaranga peltata Roots and Screening of their Cytotoxic Activity. Int J Pharm Chem Sci. 2014;3(2):415–419.
Malek SNA, Shin SK, Wahab NA, Yaacob H. Cytotoxic components of Pereskia bleo (Kunth) DC. (Cactaceae) leaves. Molecules. 2009;14(5):1713–1724.
Bao X, Zhang Y, Zhang H, Xia L. Molecular Mechanism of β-Sitosterol and its Derivatives in Tumor Progression. Front Oncol. 2022;12:1–12.
Alvarez-Sala A, Attanzio A, Tesoriere L, Garcia-Llatas G, Barberá R, Cilla A. Apoptotic effect of a phytosterol-ingredient and its main phytosterol (β-sitosterol) in human cancer cell lines. Int J Food Sci Nutr. 2018;70(3):323–334.