Cytotoxic and Molecular Docking Potential of β-Sitosterol Isolated from <I>Lantana camara</I> Leaves against Breast (T47D) and Cervical Cancer (HeLa) Cell Lines�

Suryati; Adlis Santoni; Rahmi V. Ulia; Imelda

doi:10.26538/tjnpr/v8i4.23

Authors

Suryati Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, West Sumatra, Indonesia, 25163
Adlis Santoni Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, West Sumatra, Indonesia, 25163
Rahmi V. Ulia Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, West Sumatra, Indonesia, 25163
Imelda Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, West Sumatra, Indonesia, 25163

DOI:

https://doi.org/10.26538/tjnpr/v8i4.23

Keywords:

Microculture tetrazolium assa, molecular docking, HeLa cell, T47D cell, Lantana camara

Abstract

Lantana camara is a plant used in traditional medicine to treat various diseases.The cytotoxic potential ofÂ Lantana camara leaves has been reported for extracts and isolated pure compounds. So this study aims to test the cytotoxic potential ofÎ²-sitosterol isolated from the leaves ofLantana camarausingin silico (Molecular docking) as well asin-vitro MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against T47D and HeLa cancer cells. In this study, the isolation ofÎ²-sitosterol from the ethyl acetate extract ofÂ Lantana camara leaves was carried out by gravity column chromatography and purification by recrystallisation. The isolated compound was obtained as a white amorphous solid with a melting point of 136-137 Â°C. The structure of the isolated compound was determined by extensive IR,1H-NMR, and13C-NMR spectra data analysis and with comparison to literature data. The molecular docking results forÎ²-sitosterol showed good docking score values of -8.11 kcal/mol against Bcl- 2 and -7.276 kcal/mol against HPV16 E7, as well as a Root Mean Score Deviation (RMSD) value â‰¤ 2 compared to the standard anticancer drug doxorubicin, -9.230 kcal/mol against Bcl-2 and -8.288 kcal/mol (HPV16 E7), respectively. The results of the potential cytotoxic test ofÎ²-sitosterol using the MTT method showed its high cytotoxic properties against T47D (breast cancer cell line) and HeLa (cervical cancer cell line) with IC50 values of 9.98 Âµg/mL and 10.31 Âµg/mL,respectively.

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