HPLC-DAD Analysis and Antioxidant Potential of <i>Ferula assa foetida</i> Resin Ethanol Extract


  • Hichem Beladjal Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli, Mascara, 29000, Algeria
  • Djilali Bouhadi Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli, Mascara, 29000, Algeria
  • Hamza Belkhodja Laboratory of Bioconversion, Microbiology Engineering and Health Safety, University of Mustapha Stambouli, Mascara, 29000, Algeria




Polyphenols, Oxidative stress, Ferula assa foetida resin, Extraction, Antioxidant


Ferula assa foetida, commonly consumed as a healthy beverage, has been demonstrated to have various biological activities, including antioxidant activity. The present study aims to investigate the chemical composition and the antioxidant effect of F. assa foetida resin ethanol extract. Qualitative phytochemical screening of the extract was done according to standard procedure. The chemical constituents of F. assa foetida resin ethanol extract was identified using high performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry (HPLC-DAD-ESI-MS) analysis. The antioxidant potential was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and total antioxidant capacity assays. Qualitative phytochemical analysis revealed the presence of various bioactive phytochemicals including steroids, saponins, flavonoids, phenols, coumarins, and cardiac glycosides. Eleven compounds were identified from the HPLC analysis of the ethanol extract of F. assa foetida resin. These include vanillic acid, p-coumaric acid, ferulic acid, 3,4-dihydroxybenzoic acid, chlorogenic acid, sinapic acid, caffeic acid derivative, chrysin, rosmarinic acid, umbelliprenin and galbanic acid. The extract exhibited high DPPH free radical scavenging activity with a percentage inhibition of 96.5% against 98.3% for ascorbic acid. In addition, the ethanol extract of F. assa foetida displayed a moderate total antioxidant capacity with a value of 3.31 mg AAE/g. On the basis of the findings from this study, Ferula assa foetida resin extract could therefore be considered as an alternative source of antioxidant agent that can prevent against oxidant stress-induced damage.


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How to Cite

Beladjal, H., Bouhadi, D., & Belkhodja, H. (2024). HPLC-DAD Analysis and Antioxidant Potential of <i>Ferula assa foetida</i> Resin Ethanol Extract. Tropical Journal of Natural Product Research (TJNPR), 8(4), 6906–6910. https://doi.org/10.26538/tjnpr/v8i4.22