Antiplasmodial Activity of Ethanol Extract of Sonneratia alba Leaves
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Abstract
Sonneratia alba (S. alba) is a mangrove plant that represents a promising natural source for
potential antimalarial drug development. This study investigates the antimalarial effects of the
ethanol extract derived from S. alba leaves in a mouse model. To assess the antimalarial activity
of the extracts in vivo, mice were intraperitoneally injected with the Plasmodium berghei ANKA
strain, followed by daily administration of the ethanol extract from S. alba leaves for four
consecutive days. Parasitemia levels were monitored using light microscopy. The findings
revealed a significant suppression of parasitemia in malaria-infected mice treated with the ethanol
extract of S. alba leaves at a dose of 300 mg/kg BW, achieving a reduction of 77.34%. Mice
administered with 150 mg/kg BW of the ethanol extract demonstrated a suppression rate of
62.35%, while those treated with 75 mg/kg BW displayed 43.53% suppression. This study
underscores the potential antimalarial properties of the ethanol extract derived from S. alba leaves
when administered in vivo.
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References
Phuwajaroanpong A, Chaniad P, Horata N, Muangchanburee S, Kaewdana K, Punsawad C. In vitro and in vivo antimalarial activities and toxicological assessment of Pogostemon cablin (Blanco) Benth. J. Evidence-Based Integrative Med. 2020;25:1-11.
Chaniad P, Techarang T, Phuwajaroanpong A, Na-ek P,Viriyavejakul P, Punsawad C. In vivo antimalarial activity and toxicity study of extracts of Tagetes erecta L. and Synedrella nodiflora (L.) Gaertn. from the Asteraceae Family. Evidence-Based Comple & Alterna Med. 2021; 1; 1-9.
Peters W, Portus JH, Robinson BL. The chemotherapy of rodent malaria, XXII. Annals of Trop Med & Parasit. 1975; 69(2):155–171.
Chaerunisaaa AY, Susilawati Y, Muhaimin M, Milanda T, Hendriani R, Subarnas A. Antibacterial activity and subchronic toxicity of Cassia fistula L. barks in rats. Toxicol Rep. 2020; 7:649-657.
Retnosari D, Purnobasuki H, Supriyanto A. Antimalarial Activity of Crude Bark Extract of Pterocarpus indicus Willd. against Plasmodium falciparum Strain 3D7. Trop J Nat Prod Res. 2023; 7(9):3893-3897. http://www.doi.org/10.26538/tjnpr/v7i9.6.
Muhaimin, Yusnaidar, Syahri W, Latief M, Utami A, Bemis R, Amanda H, Heriyanti, Chaerunisaa AY. Screening and potential analysis of methanolic leaf extract of Mangrove plants at east coast Sumatera as repellent against Aedes aegypti. J Pharm Sci Res. 2018; 10(9): 2228-2231.
Fadipe LA, Ajemba C, Lawal BA, Ahmadu AA, Ibikunle GF. Phytochemical and In-vivo Antimalarial Investigations of Dichrostachys cinerea (L.) Wight & Arn. (Fabaceae) Root Bark. Trop J Nat Prod Res. 2020; 4(11):1007-1014. doi.org/10.26538/tjnpr/v4i11.28.
Habibi P, Shi Y, Fatima Grossi-de-Sa M, Khan I. Plants as Sources of Natural and Recombinant Antimalaria Agents. Mol Biotechnol. 2022; 64(11):1177-1197.
Berthi W, González A, Rios A, Blair S, Cogollo Á, Pabón A. Anti-plasmodial Fffect of Plant Extracts from Picrolemma huberi and Picramnia latifolia. Malar J. 2018; 17(1):151.
Uzor PF. Alkaloids from Plants with Antimalarial Activity: A Review of Recent Studies. Evid Based Complement Alterna Med. 2020; 20(1):1-17.
Syafrizal, Ramadhan R, Kusuma IW, Egra S, Shimizu K, Kanzaki M, Arung ET. Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiv J Biologic Div, 2020; 21(10):4623-4630.
Shija KM, Nondo RSO, Mloka D, Sangeda RZ, Bwire GM. Effects of Lemon Decoction on Malaria Parasite Clearance and Selected Hematological Parameters in Plasmodium berghei ANKA Infected Mice. BMC Complement Med Ther. 2020; 20(1):24.
Ihekwereme CP, Melidem CO, Maduka IC, Okoyeh JN. In Vivo Antiplasmodial and Toxicological Effects of Extracts of Fruit Pulp of Chrysophyllum albidium G. Don (Sapotaceae). Trop J Nat Prod Res. 2018; 2(3):126-131. doi.org/10.26538/tjnpr/v2i3.5.
Ojo SKS, Udewena LU, Durodola OT, Olarinoye OO, Ariyo OO, Herbert SJ, Lawal AM. Antibacterial Activity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus. Trop J Nat Prod Res. 2024; 8(2):6457-6464. http://www.doi.org/10.26538/tjnpr/v8i2.39.
Laksemi DA, Sukrama ID, Suwanti LT, Sudarmaja M, Damayanti PA, Tunas IK, Wiryanthini IA, Linawati NM. A Comprehensive Review on Medicinal Plants Potentially as Antimalarial. Trop J Nat Prod Res, 2022; 6(3), 287–298.
Al-Aamri MS, Al-Abousi NM, Al-Jabri SS, Alam T, Khan SA. Chemical Composition and in Vitro Antioxidant and Antimicrobial Activity of the Essential Oil of Citrus aurantifolia L. Leaves grown in Eastern Oman. J Taibah Univ Med Sci. 2018; 13(2):108-112.
Uchenna BA, Abdullahi M, Yusuf KA, Olofu OE. Antimalarial activity of crude extract and fractions of Phyllanthus amarus in Plasmodium berghei infected mice. Eur J Med Plants. 2018; 24: 1-11.
Muhaimin, Syamsurizal, Chaerunisaa AY, Sinaga MS. Eusiderin I from Eusideroxylon zwagery as antifungal agent against plant pathogenic fungus. Int J ChemTech Res. 2016; 9(5): 418-424.
Chaerunisaa AY, Muhaimin. Comparative study on release of two drugs in fixed dose combination using zeroorder and first derivative spectrophotometry. Int J PharmTech Res. 2016; 9(12): 581-590.
Mulenga MC, Sitali L, Ciubotariu II, Hawela MB, Hamainza B, Chipeta J, Mharakurwa S. Decreased prevalence of the Plasmodium falciparum Pfcrt K76T and Pfmdr1 and N86Y mutations post-chloroquine treatment withdrawal in Katete District, Eastern Zambia. Malaria J. 2021; 20: 329.
Taek MM, Tukan GD, Prajogo BEW, Agil M. Antiplasmodial activity and phytochemical constituents of selected antimalarial plants used by native people in West Timor Indonesia. Turkish J Pharm Sci. 2021; 18(1):80-90.
Nwonuma CO, Balogun EA, Gyebi GA. Evaluation of antimalarial activity of ethanolic extract of Annona muricata L.: an in vivo and an in silico Approach. J Evid Based Integr Med. 2023;28: 1-10.
Abouelela ME, Abdelhamid RA, Orabi MAA. Phytochemical constituents of plant species of Pterocarpus (F: Leguminosae): a review. Int J Pharmacogn Phytochem Res. 2019; 11(4): 264-281.
WHO. Malaria world report. World Health Organization, Geneva, Switzerland: WHO Press; 2019; 1-50.
Czechowski T, Rinaldi MA, Famodimu MT, Veelen MV. Larson TR. Thilo W. Rathbone DA. Harvey D. Horrocks P. Graham IA. Flavonoid versus Artemisinin Anti-malarial Activity in Artemisia annua Whole-Leaf Extracts. Front Plant Sci. 2019; 10(984):1-11.
Hailesilase GG, Rajeshwar Y, Hailu GS, Sibhat GG, Bitew H. In vivo antimalarial evaluation of crude extract, solvent fractions, and TLC-isolated compounds from Olea europaea Linn subsp. cuspidata (Oleaceae). Evid Based Complement Alter Med. 2020: 15-20.
Saivaraj S. Antimicrobial activity of natural dyes obtained from Pterocarpus indicus Wild. barks. Asian J Pharm Res Dev. 2018; 6(2): 6-8.
Abdussalam US, Allyu M, Maje IM. In vivo antiplasmodial activity of ethanol leaf extract of Marrubium vulgare L. (Lamiaceae) in Plasmodium berghei-berghei infected mice. Trop J Nat Prod Res. 2018; 2(3): 132-135.
Altharawi A, Riadi Y, Tahir Ul Qamar M. An in silico quest for next-generation antimalarial drugs by targeting Plasmodium falciparum hexose transporter protein: a multipronged approach. J Biomol Struct Dyn. 2023;1–10.
Ogbeide OK, Dickson VO, Jebba RD, Owhiroro DA, Olaoluwa MO, Imieje VO, Erharuyi O, Owolabi BJ, Fasinu PS, Falodun A. Antiplasmodial and acute toxicity studies of fractions and cassane-type diterpenoids from the stem bark of Caesalpinia pulcherrima (L.) Sw. Trop J Nat Prod Res. 2018; 2(4): 179-184.
Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro antiinflammatory activities of Severinia buxifolia. J Food Qua. 2019; 19(1): 1-10.
Pippione AC, Sainas S, Goyal P, Fritzson I, Cassiano GC, Giraudo A, Giorgis M, Tavella TA, Bagnati R, Rolando B, Caing-Carlsson R, Costa FTM, Andrade CH, Al-Karadaghi S, Boschi D, Friemann R, Lolli ML. Hydroxyazole scaffoldbased Plasmodium falciparum dihydroorotate dehydrogenase inhibitors: Synthesis, biological evaluation and X-ray structural studies. Eur J Med Chem. 2019;163:266–80.
Owoloye AJ, Ligali FC, Enejoh OA, Musa AZ, Aina O, Idowu ET, Oyebola KM. Molecular docking, simulation and binding free energy analysis of small molecules as PfHT1 inhibitors. PloS One. 2022;17(8):e0268269.
Czechowski T, Rinaldi MA, Famodimu MT, Veelen MV. Larson TR. Thilo W. Rathbone DA. Harvey D. Horrocks P. Graham IA. Flavonoid versus Artemisinin Anti-malarial Activity in Artemisia annua Whole-Leaf Extracts. Front Plant Sci. 2019; 10(984):1-11.
Tajuddeen N, Van Heerden FR. Antiplasmodial natural products: an update. Malaria J 2019; 18(1): 404.
Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro antiinflammatory activities of Severinia buxifolia. J Food Qua. 2019; 1-15.
Siddiqui FA, Boonhok R, Cabrera M, Mbenda HGN, Wang M, Min H, Liang X, Qin J, Zhu X, Miao J, Cao Y, Cui L. Role of Plasmodium falciparum Kelch 13 Protein Mutations in P. falciparum Populations from Northeastern Myanmar in Mediating Artemisinin Resistance. mBio. 2020;11(1):11-19.
Heller LE, Roepe PD. Quantification of Free Ferriprotoporphyrin IX Heme and Hemozoin for Artemisinin Sensitive versus Delayed Clearance Phenotype Plasmodium falciparum Malarial Parasites. Biochemistry. 2018;57(51):6927–6934.
Heller LE, Goggins E, Roepe PD. Dihydroartemisinin Ferriprotoporphyrin IX Adduct Abundance in Plasmodium falciparum Malarial Parasites and the Relationship to Emerging Artemisinin Resistance. Biochemistry. 2018;57(51):6935–6945.
Ross LS, Lafuente-Monasterio MJ, Sakata-Kato T, Mandt REK, Gamo FJ, Wirth DF, Lukens AK. Identification of Collateral Sensitivity to Dihydroorotate Dehydrogenase Inhibitors in Plasmodium falciparum. ACS Infect Dis. 2018;4(4):508–515.
Chagas CM, Moss S, Alisaraie L. Drug metabolites and their effects on the development of adverse reactions: Revisiting Lipinski’s Rule of Five. Int J Pharm. 2018;549(1–2):133–49.
Boschi D, Pippione AC, Sainas S, Lolli ML. Dihydroorotate dehydrogenase inhibitors in anti-infective drug research. Eur J Med Chem. 2019;183:111-118.
Muhaimin M, Yusnaidar Y, Syahri W, Latief M, Putri RD, Utami A, et al. Antiplasmodial Activity of Ethanolic Extract of Macaranga Gigantea Leaf and Its Major Constituent. Pharmacog J. 2019;11(6):1181-1188.
Chaniad P, Techarang T, Phuwajaroanpong A, Na-ek P, Viriyavejakul P, Punsawad C. In Vivo Antimalarial Activity and Toxicity Study of Extracts of Tagetes erecta L. and Synedrella nodiflora (L.) Gaertn. from the Asteraceae Family. Evidence-Based Complemen & Alternat Med. 2021; 902-912.
Nsanzabana C. Resistance to artemisinin combination therapies (ACTs): do not forget the partner drug. Trop Med & Infect Dis. 2019; 4(1):26.
Juhairiyah J, Andiarsa D, Indriyati L, Ridha MR, Prasodjo RS, Dhewantara PW. Spatial analysis of malaria in Kotabaru, South Kalimantan, Indonesia: an evaluation to guide elimination strategies. Trans R Soc Trop Med Hyg. 2021;115(5):500–511.
Carmichael N, Day PJR. Cell Surface Transporters and Novel Drug Developments. Front Pharmacol. 2022;13: 938-946.
Muhaimin M, Yusnaidar Y, Syahri W, Latief M, Chaerunisaa AY. Microencapsulation of Macaranga gigantea Leaf Extracts: Production and Characterization. Pharmacog. J. 2020; 12 (4):716-724.
Astuti P, Rollando R, Wahyuono S, Nurrochmad A. Antimicrobial activities of isoprene compounds produced by an endophytic fungus isolated from the leaves of Coleus amboinicus Lour. J Pharm Pharmacogn Res. 2020;8(4):280– 9.
Okokon JE, Antia BS, Mohanakrishnan D, Sahal D. Antimalarial and antiplasmodial activity of husk extract and fractions of Zea mays. Pharm Biol. 2017; 55(1): 1394–1400.
Hariono M, Julianus J, Djunarko I, Hidayat I, Adelya L, Indayani F, Auw Z. Namba G, Hariyono P. The Future of Carica papaya Leaf Extract as an Herbal Medicine Product. Mol Basel Switz. 2021;26(22):6922.
Muhaimin, Yusnaidar, Syahri W, Latief M, Utami A, Bemis R, et al. Screening and potential analysis of methanolic leaf extract of Mangrove plants at east coast Sumatera as repellent against Aedes aegypti. J Pharm Sci Res. 2018;10(9):2228-31