Phytochemical Composition, Antibacterial Activity and Acute Toxicity Studies of Euphorbia resinifiera O. Berg


  • Basma Aghoutane Laboratory of Plant Chemistry and Organic and Bio-Organic Synthesis. Faculty of Sciences, Mohammed-V University of Rabat, Morocco.
  • Amal Naama Laboratory of Plant Chemistry and Organic and Bio-Organic Synthesis. Faculty of Sciences, Mohammed-V University of Rabat, Morocco.
  • Imane El Attar Microbiology and Molecular Biology Unit, Plant and Microbial Biotechnology, Biodiversity and Environment Center, Faculty of Sciences, University Mohamed V, Rabat, Morocco.
  • Otman El-Gourrami Laboratory of Analytical Chemistry and Bromatology, Faculty of Medicine and Pharmacy of Rabat Mohamed V, University of Rabat, Morocco.
  • Hanae El Monfalouti Laboratory of Plant Chemistry and Organic and Bio-Organic Synthesis. Faculty of Sciences, Mohammed-V University of Rabat, Morocco.
  • Badr Eddine Kartah Laboratory of Plant Chemistry and Organic and Bio-Organic Synthesis. Faculty of Sciences, Mohammed-V University of Rabat, Morocco.



Euphorbia resinifiera, Extraction Method, Antibacterial Activity, Alkaloids, Acute Toxicity


Euphorbia resinifiera is a medicinal plant exploited by the native population to treat various diseases. The study compared the effects of seven different extracts of Euphorbia resinifiera by evaluating their antibacterial activity. The study reported the alkaloid percentage, total protein content, carotenoid content, and tannin content in the aerial part of Euphorbia resinifiera. The antibacterial activity of the seven extracts (100 mg/mL/disc) was evaluated by disc diffusion method against six pathogenic bacteria: 2 Gram-positive (Staphylococcus aureus and Microbacteriumresistant) and 4 Gram-negative (Klebsiella pneumonia, Escherichia coli,  Alcaligenesfaecalis, and pseudomonas chloritidismutans). Aqueous methanol extract obtained by maceration showed the best antibacterial activity against all tested bacteria. Whereas the percentage of total protein content was very low, 1.28%, and the alkaloid yield was 0.08±0.013 g/100 g of dry weight. The results revealed that the lethal dose (LD50) was higher than 300 mg/kg for the aqueous extract obtained by maceration. For other extracts, the LD50 was higher than 2000 mg/mL (maceration methanol, maceration Methanol-water, sonication methanol, sonication water, sonication Methanol-water, infusion.). Overall, the results indicated that Euphorbia resinifiera possesses antibacterial activity against the isolates studied.


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How to Cite

Aghoutane, B., Naama, A., Attar, I. E., El-Gourrami, O., Monfalouti, H. E., & Kartah, B. E. (2024). Phytochemical Composition, Antibacterial Activity and Acute Toxicity Studies of Euphorbia resinifiera O. Berg. Tropical Journal of Natural Product Research (TJNPR), 8(4), 6814–6819.