Gas Chromatography Analysis, Mineral Contents and Anti-inflammatory Activity of Sonchus maritimus

Authors

  • Sara Chetehouna Faculty of Sciences, University of M’sila, PO Box 166 Ichebilia, 28000 M’sila, Algeria
  • Samir Derouiche Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences, El Oued University, El Oued 39000, Algeria
  • Yassine Réggami Department of Natural and Life Sciences, Faculty of Sciences, University 20 August 1955 - Skikda, SKIKDA 21000, Algeria
  • Islam Boulaares Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences, El Oued University, El Oued 39000, Algeria
  • Ahlem Frahtia Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences, El Oued University, El Oued 39000, Algeria

DOI:

https://doi.org/10.26538/tjnpr/v8i4.7

Keywords:

Sonchus maritimus, GC analysis, Phytochemical screening, Phenols, flavonoids

Abstract

Sonchus maritimus belongs to Asteraceae family. It is a well-known medicinal plant for its phytochemicals and therapeutic values. This paper aimed to investigate the phytochemicals, Gas chromatography analysis of leaves aqueous extract of Sonchus maritimus and to estimate its anti inflammatory propriety. Quantitative and qualitative phytochemical including GC analysis were carried out using studied protocols. Anti-inflammatory activity of extract was also evaluated using inhibition of protein denaturation and anti-hemolysis assays. The phytochemical screening of the leaves aqueous extract of S. maritimus demonstrated the presence of phenols, flavonoids, tannins, terpenoids, sponins, unsaturated steroids and derived steroids whereas the absence of alkaloids and reducing compounds. The results of quantitative analysis showed that S. maritimus has

significant amounts of flavonoids and condensed tannins in addition to a reasonable amount of phenols. In addition to important content of phytominerals including sodium, potassium, calcium, iron and magnesium, copper, zinc and manganese. GC results showed that the leaves extract of S. maritimus contained a large number of different molecules, among abundant volatile compounds; 1-Hexanol, 2-ethyl-, Ethylene glycol - Adipate - Diethylene glycol, Cyclotrisiloxane, and hexamethyl. The extract of S. mritimus was exhibited an important activity to inhibit the albumin denaturation (IC50 334.369±29 µg/mL) and to protect erythrocytes against hemolysis (IC50 53.539 ± 4.64 µg/ mL). In conclusion, the abundance of volatile phytocompounds in the leaves aqueous extract of S. maritimus could indicate distinct biological activities while the therapeutic molecules are used as new pharmaceutical products to treat diverse diseases in the future

Author Biographies

Sara Chetehouna, Faculty of Sciences, University of M’sila, PO Box 166 Ichebilia, 28000 M’sila, Algeria

Laboratory of Biology: Applications in Health and Environment, Faculty of Sciences, University of M’sila, PO Box 166 Ichebilia, 28000 M’sila, Algeria.

Samir Derouiche, Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences, El Oued University, El Oued 39000, Algeria

Laboratory of Biodiversity and application of biotechnology in the agricultural field, Faculty of natural and life sciences, El Oued University, El-Oued 39000, Algeria

Yassine Réggami, Department of Natural and Life Sciences, Faculty of Sciences, University 20 August 1955 - Skikda, SKIKDA 21000, Algeria

Laboratory of Biology: Applications in Health and Environment, Faculty of Sciences, University of M’sila, PO Box 166 Ichebilia, 28000 M’sila, Algeria

Islam Boulaares, Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences, El Oued University, El Oued 39000, Algeria

Laboratory of Biodiversity and application of biotechnology in the agricultural field, Faculty of natural and life sciences, El Oued University, El-Oued 39000, Algeria

Ahlem Frahtia, Department of Cellular and Molecular Biology, Faculty of Natural and Life Sciences, El Oued University, El Oued 39000, Algeria

Laboratory of Biodiversity and application of biotechnology in the agricultural field, Faculty of natural and life sciences, El Oued University, El-Oued 39000, Algeria

References

Pant DR, Pant ND, Saru DB, Yadav UN, Khanal DP. Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh. J Intercult Ethnopharmacol.

;6(2):170-176. Doi:10.5455/jice.20170403094055

Liu A-L, Wang Y-H, Wang T-Y, Zhu Y, Wu P, Li L-J. Comparative metabolomic profiling of secondary metabolites in different tissues of Euryale ferox and

functional characterization of phenylalanine ammonia-lyase. Ind Crops Prod. 2023;195:116450.

Doi:https://doi.org/10.1016/j.indcrop.2023.116450

Fouad H, Habib ES, Ahmed SA. Phytochemistry and Pharmacological Effects of Plants in Genus Sonchus

(Asteraceae). Rec Pharm Biomed Sci. 2020;4(1):40-50.

Doi:10.21608/RPBS.2019.18952.1046

Elhady SS, Abdelhameed RFA, Mehanna ET, Wahba AS, Elfaky MA, Koshak AE, Noor AO, Bogari HA, Malatani RT, Goda MS. Metabolic Profiling Chemical Composition

Antioxidant Capacity and In Vivo Hepato- and Nephroprotective Effects of Sonchus cornutus in Mice Exposed to Cisplatin. Antioxidants. 2022;11(5):1-26.

Doi:10.3390/antiox11050819

Hameed AT, Al-alh NMA, Jumaa AW. Antioxidant Activity and Phytominerals Study of Some Asteraceae Species

Growth in Western of Lraq. Indian J Forensic Med Toxicol. 2021;15(1):2239-2245. Doi:10.37506/ijfmt.v15i1.13736

Mohasib RMM, Nagib A, Samad AA, Salama ZA, Gaafar AA. Identification Of Bioactive Ingredients IN Sonchus

Oleraceus by HPLC and GC / MS. Plant Arch. 2020;20(2):9714-9720.

Nwozo OS, Effiong EM, Aja PM, Awuchi CG. Antioxidant, phytochemical, and therapeutic properties of medicinal

plants: a review. Int J Food Prop. 2023;26(1):359-388.

Doi:10.1080/10942912.2022.2157425

Al-Khayri JM, Banadka A, Nandhini M, Nagella P, AlMssallem MQ, Alessa FM. Essential Oil from Coriandrum sativum: A review on Its Phytochemistry and Biological Activity Molecules. 2023;28(2):1-23. Doi:10.3390/molecules28020696

Derouiche S, Abbas K, Djermoune M. Polysaccharides and Ascorbic Acid Content and the Effect of Aqueous Extract of

Portulaca Oleracea in High-Fat Diet-Induced Obesity, Dyslipidemia and Liver Damage in Albino Wistar Rats. Alger J Arid Environ. 2017;7(2):16-26. Doi:10.12816/0046095

Evans WC. Trease and Evans’ Pharmacognosy. 16th ed. Elsevier Health Sciences, London; 2009.

Harborne AJ. Phytochemical Methods a Guide to Modern Techniques of Plant Analysis. springer science & business

media; 1998.

Wadood A. Phytochemical Analysis of Medicinal Plants Occurring in Local Area of Mardan. Biochem Anal Biochem. 2013;02(04):2-5. Doi:10.4172/21611009.1000144

Harborne JB. Phenolic compounds. Phytochem methods A Guid to Mod Tech plant Anal. 1973:33-88.

Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic. 1977;28(1):49-55. Doi:10.5344/ajev.1977.28.1.49

Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T. Antioxidant activity and constituents of

propolis collected in various areas of China. Food Chem. 2007;101(4):1383-1392.

Doi:10.1016/j.foodchem.2006.03.045

Broadhurst RB, Jones WT. Analysis of condensed tannins using acidified vanillin. J Sci Food Agric. 1978;29(9):788-794. Doi:10.1002/jsfa.2740290908

Vinjamuri S, Afshan S, Shekar S, Saraswathi VJ. Evaluation of hemolytic activity, atpase inhibitory activity and antitumor activity of TLC extract of lemon grass (Cymbpogon citratus). Int J Pharmacogn Phytochem Res.

;7(4):785-788.

Vennila K, Chitra L, Balagurunathan R, Palvannan T. Comparison of biological activities of selenium and silver

nanoparticles attached with bioactive phytoconstituents: Green synthesized using Spermacoce hispida extract. Adv

Nat Sci Nanosci Nanotechnol. 2018;9(1):1-10. Doi:10.1088/2043-6254/aa9f4d

El-akhal F, Alami A, Chahmi N, MouatassemFilaliT, El Fattouhi Y, Benboubker M, Amaiach R, Benrezzouk R,

Taghzouti K, Talbi FZ, El Ouali Lalami A.

Phytochemical Screening, Chemical Composition and Larvicidal Efficacy of Syzygium aromaticum Extracts and

Essential Oil against Culex pipiens. Trop J Nat Prod Res. 2024; 8(1):5962-

http://www.doi.org/10.26538/tjnpr/v8i1.35

Herdiana N, Hidayati S, Sartika D, Subeki, Suharyono, Yuliandari P, Zuidar AS. Identification of Bioactive Compounds and Antibacterial Activity of Limeberry

(Triphasia trifolia) Fruit Ethanol Extract against Staphylococcus aureus. Trop J Nat Prod Res. 2024; 8(1):5820-5824.http://www.doi.org/10.26538/tjnpr/v8i1.16.

Ayoola GA, Folawewo AD, Adesegun SA, Abioro OO. Phytochemical and antioxidant screening of some plants of apocynaceae from South West Nigeria. Int J Plant Breed

Genet. 2019;6(4):1-5.

Soldado D, Bessa RJB, Jerónimo E. Condensed tannins as antioxidants in ruminants-effective-ness and action

mechanisms to improve animal antioxidant status and oxidative stability of products. Animals. 2021;11(11):1-25.

Doi:10.3390/ani11113243

Petropoulos S, Karkanis A, Martins N, Ferreira ICFR. Phytochemical composition and bioactive compounds of common purslane (Portulaca oleracea L.) as affected by crop management practices. Trends food Sci Technol. 2016;55:1-10. Doi:10.1016/j.tifs.2016.06.010

Benmohamed M, Guenane H, Messaoudi M, et al. Mineral Profile, Antioxidant, Anti-Inflammatory, Antibacterial, Anti-Urease and Anti-α-Amylase Activities of the Unripe Fruit Extracts of Pistacia atlantica. Molecules.

;28(1):1-20. Doi:10.3390/molecules28010349

Neethu BK, Yathiender S. Phytochemical Screening and GC- MS Analysis of Three Indian Traditional Medicinal Plants. Curr Trends Biotechnol Pharm. 2023;17:1097-1105.

Doi:10.5530/ctbp.2023.3s.48

Ahmad F, Abdallah ET, Kamil M. Scientific studies on aerial parts of Sonchus oleraceus linn. Arab J Med Aromat Plants.

;7(2):196-214. Doi:10.48347/IMIST.PRSM/ajmapv7i2.26287

Cruz AF, Hamel C, Yang C, Matsubara T, Gan Y, Singh AK, Kuwada K, Ishii T. Phytochemicals to suppress Fusarium

head blight in wheat-chickpea rotation. Phytochemistry. 2012;78:72-80. Doi:10.1016/j.phytochem.2012.03.003

Helal NM, Ibrahim NA, Khattab H. Phytochemical analysis and antifungal bioactivity of Pulicaria undulata (L.)

methanolic extract and essential oil. Egypt J Bot. 2019;59(3):827-844. Doi:10.21608/ejbo.2019.12259.1308

Gaafar AA, Ali SI, Kutkat O, Kandeil AM, El-Hallouty SM. Bioactive Ingredients and Anti-influenza (H5N1), Anticancer, and Antioxidant Properties of Urtica urens L.

Jordan J Biol Sci. 2020;13:647-657.

Juariah S, Juliantari E, Dika Hanggara D, Surya A, Endrini S. Chemical Profiling, Antifungal and Antiinflammatory Evaluations of Ethanol Extract of Zingiber

officinale var. rubrumand Curcuma caesia Mixture from Riau, Sumatera Island, Indonesia. Trop J Nat Prod Res. 2024;

(1):5794-5800.http://www.doi.org/10.26538/tjnpr/v8i1.12

Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro antiinflammatory activities of severinia buxifolia. J Food Qual. 2019;2019:1-9. Doi:10.1155/2019/8178294

Helmstadter A. Ethnopharmacology in the work of Melville William Hilton-Simpson (1881-1938) - Historical analysis

and current research opportunities. Pharmazie. 2016;71(6):352-360. Doi:10.1691/ph.2016.6533

Downloads

Published

2024-05-01

How to Cite

Chetehouna, S., Derouiche, S., Réggami, Y., Boulaares, I., & Frahtia, A. (2024). Gas Chromatography Analysis, Mineral Contents and Anti-inflammatory Activity of Sonchus maritimus. Tropical Journal of Natural Product Research (TJNPR), 8(4), 6787–6798. https://doi.org/10.26538/tjnpr/v8i4.7