Antiproliferative Potential of Ethanol Leaf Extract of <i>Motandra guineensis</i> (Thonn.) A.DC. (Apocynaceae) against Human Melanoma and Ovarian Cancer Cells

Authors

  • Joseph O. Oiseoghaede Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Idi-Araba, Surulere, Lagos, Nigeria
  • Aminat A. Oyawaluja Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Idi-Araba, Surulere, Lagos, Nigeria
  • Abimbola A. Sowemimo Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Idi-Araba, Surulere, Lagos, Nigeria
  • Olukemi A. Odukoya Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Idi-Araba, Surulere, Lagos, Nigeria
  • Chun-Tao Che Department of Pharmaceutical Sciences, College of Pharmacy, University of Illinois, Chicago, Chicago 60612, Illinois, USA

DOI:

https://doi.org/10.26538/tjnpr/v8i3.33

Keywords:

murine macrophage cells, Vero cells, Human melanoma skin cancer, Ovarian cancer, Motandra guineensis, nitric oxide inhibition, Antiproliferative

Abstract

In ethnomedicine, Motandra guineensis (Thonn.) A.DC. (Apocynaceae) is used to massage abscessed gums and manage pain. This study aimed to evaluate the antiproliferative effect of leaves of M. guineensis against human ovarian (OVCAR3) and melanoma (MDA-MB-435) skin cancer cells. Crude ethanol extract and fractions of M. guineensis leaves were evaluated for antiproliferative effect on OVCAR3 and MDA-MB-435 cells using cell viability assay at 2 and 20 µg/ml. In addition, the inhibitory effect of extract and fractions on nitric oxide (NO) production with lipopolysaccharide (LPS) stimulation (Greiss assay) on murine macrophage cells (RAW 264.7) at 0.3125 – 10 µg/ml was evaluated. Cell viability was evaluated using murine macrophage cells while toxicity assessment was done on Vero cells. Antiproliferative activity profile (% cell death) of extract and fractions followed the order: butanol (30% and 23%) > aqueous (28% and 21%) > ethyl acetate (22% and 19%) > crude (22% and 0%) at 20 μg/ml in OVCAR3 and MDA-MB-435 cell lines respectively. Percentage NO inhibitory activity showed hexane fraction with the highest activity and aqueous fraction with the least activity at 10 μg/ml. Extract and fractions were less toxic in Vero cells when compared to the standard drug, Tamoxifen. Toxicity assessment using murine macrophage cells showed no significant difference in cell viability when compared to standard. Results indicate antiproliferative potential, NO inhibitory potential and safety of crude ethanol leaf extract and fractions of M. guineensis.

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Published

2024-03-30

How to Cite

Oiseoghaede, J. O., Oyawaluja, A. A., Sowemimo, A. A., Odukoya, O. A., & Che, C.-T. (2024). Antiproliferative Potential of Ethanol Leaf Extract of <i>Motandra guineensis</i> (Thonn.) A.DC. (Apocynaceae) against Human Melanoma and Ovarian Cancer Cells. Tropical Journal of Natural Product Research (TJNPR), 8(3), 6700–6704. https://doi.org/10.26538/tjnpr/v8i3.33