Effect of <i>Pterocarpus santalinus</i> Ethanol Leaf Extract on Haematological, Histopathological and Lipid Profile Indices in Wistar Rats


  • Joseph S. Oyepata Departmennt of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Federal University, Oye-Ekiti, Ekiti State, Nigeria
  • Joseph T. Opeyemi Department of Pharmacology, Faculty of Pharmacy, Lead City University, Ibadan, Nigeria
  • Adegbuyi T. Adekunle Departmennt of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Federal University, Oye-Ekiti, Ekiti State, Nigeria.
  • Omoirri A. Moses Departmennt of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Federal University, Oye-Ekiti, Ekiti State, Nigeria.




Blood, Cardiovascular system, intestine, Pterocarpus santalinus, Wistar Rat


Pterocarpus santalinus (Red Sandalwood) is a medicinal plant used in traditional medicine for many purposes. This study is aimed at evaluating the effect of Pterocarpus santalinus leaf extract on haematological, histopathologicaland lipid profile indices in rats. Wistar rats were divided into four groups (1 – 4). Group 1 (control) was given distilled water (10 mL/kg), groups 2 - 4were administered oral doses of Pterocarpus santalinus ethanol leaf extract at 100, 200 and 400 mg/kg, respectively once daily for 28 days. The body weights of the rats were monitored on a weekly basis during the experimental period. After the last dose was administered, the rats were sacrificed, and blood samples were collected, used for haematological and lipidprofile analysis. The brain and heart of each rat were harvested and used for histopathological analysis.The extract at 100 mg/kg resulted in a decrease in the RBC, Hg, MCH, MCHC, platelets and WBC. However, there were no changes in the WBC differentials (lymphocytes, neutrophils, basophils, and eosinophils). The levels of total cholesterol and triglycerides were not altered following extract administration, but HDL-cholesterol was slightly elevated while LDL-cholesterol was reduced significantly after extract administration. Histopathological examination of the heart and intestine of the rats showed no significant differences in the histological features of the rats in the treatment groups and those in the control group. These observations suggest that Pterocarpus santalinus leaf extract may be relatively safe when used for medicinal purposes.


Pathan, MM, Khan MA,Moregaonkar SD, Somkuwar AP and Gaikwad, NZ. Amelioration of paracetamol induced nephrotoxicity by Maytenusemarginata in male wistar rats. Int. J. Pharm. Pharm. Sci. 2013, 5: 471-474.

Kang SG, Lee GB, Vinayagam R, Do GS, Oh SY, Yang SJ, Kwon JB, Singh M. Anti-Inflammatory, antioxidative, and nitric oxide-scavenging activities of a quercetinnanosuspension with polyethylene glycol in LPS-Induced RAW 264.7 Macro Mol. 2022; 27(21):7432.von Bartheld CS, Bahney J,

Herculano-Houzel S. "The search for true numbers of neurons and glial cells in the human brain: A review of 150 years of cell counting". J. Comp. Neurol. 2016; 524 (18): 3865–3895.

Charkiewicz ED, Backstrand JR. Lead Toxicity and Pollution in Poland Int J Environ Res Public Health. 2020, 17 (12), p. 4385

Eid A, Zawia N. Consequences of lead exposure, and its emerging role as an epigenetic modifier in the aging brain. ToxicolLett. 2016, 15 (296), pp. 173-183

UgboguOC Okezie E, Agi GO, Ibe C,Ekweogu CN, Ude VN. A review on the traditional uses, phytochemistry, and pharmacological activities of clove basil (Ocimumgratissimum L.). Heliyon, 7 (11) (2021), p. e08404

Oyem JC, Chris-Ozoko LE, Enaohwo MT, Otabor FO, Okudayo VA, Udi OA.Antioxidative properties of Ocimumgratissimum alters Lead acetate induced oxidative damage in lymphoid tissues and hematological parameters of adult Wistar rats. Toxicol. Rep, 8 (2021), pp. 215-222

Zubairu SA, Festus OA, Simeon JO, Irabor I, Tosin JO. Effect of Anacardiumoccidentale Fruit Juice Extract on Haematological Parameters and Spleen of Paracetamol Induced Injury in Albino Rats.Global SciJou. 2021. Volume 9, Issue 7. Page 1640-1654.

Emily M. Toxicity. In: Cutler J, (2007). editor. In: Encyclopedia of Earth. Cleveland, Washington D.C.

Arumugam G, Swamy MK, Sinniah UR. Plectranthusamboinicus (Lour.)Spreng: Botanical, phytochemical, pharmacological and nutritional significance. Molecules. 2016; 21(4):369.

Anachuna KK, Moke EG, Oyem JC, Omogbiya AI, Daubry TME, Ebuwa EI. Ethanolic Extract of OcimumGratissimum (Scent Leaf) Leaves Improves Haematological Parameters in Restrain-Stressed Rats. J ApplSci Environ Manage 2021, 25 (9), pp. 1605-1611

Dutta K, Kli M, Najam A, Kumar R. and Kumar A. Ameliorative effect of seed extract of Pterocarpussantalinus on coragen induced haematological alterations and serum biochemical changes in rats, JTEHS. 2004, 6(10): 194 – 202.

Olamilosoye KP, Akomolafe RO, Akinsomisoye OS, Adefisayo MA, Alabi QK.The aqueous extract of Ocimumgratissimum leaves ameliorates acetic acid-induced colitis via improving antioxidant status and hematological parameters in male Wistar rats. Egypt. J. Basic Appl. Sci. 2019, 5 (3), pp. 220-227.

Okechukwu N, Elizabeth F, Godwin U. Effects of aqueous extract of Ocimumgratissimum leaves and vitamin C on lead acetate-induced changes in the Thymus of adult Wistar rats effects of aqueous extract of Ocimumgratissimum leaves and vitamin C on lead acetate-induced changes in the Thymus of adult Wistar rats. IJBCCR, 26 (1) (2019), pp. 1-9

Asagba SO, Ichipi-Ifukor PC, Ichipi-Ifukor RN, J. Oyem JC. Palm oil fractions alter acute cadmium mediated haematotoxicity. Galician Med J, 26 (3) (2019), p. E201933

Duke, James. Handbook of Legumes of World Economic Importance.Springer Science & Business Media.2012. p. 49.

Balaban H, Balaban M,Nazıroğlu K, Demirci İS.The protective role of selenium on scopolamine-induced memory impairment, oxidative stress, and apoptosis in aged rats: the involvement of TRPM2 and TRPV1 channels. Mol. Neurobiol., 54 (2017), pp. 2852-2868, 10.1007/S12035-016-9835-0

Joseph OS., Jude EO and Joseph OT.Hepatoprotective activity of extract of HomaliumLetestui stem against carbon tetrachloride-induced liver injury. Adv. Herb. Med.2018;Vol 4(4), Page 1-11.

Widodo N, Puspitarini S, Widyananda MH, Alamsyah A, Wicaksono ST, Masruri M, Jatmiko YD. Anticancer activity of Caesalpiniasappan by downregulating mitochondrial genes in A549 lung cancer cell line. F1000Res. 2022; 11:169.

Ilesanmi OB, Ikpesu T. Neuromodulatory activity of trèvo on cyanide-induced neurotoxicity viz neurochemical, antioxidants, cytochrome C oxidase and p53. AdvTrad Med. 2020;21(2):297-304. https://doi.org/10.1007/s13596-020-00450-w.

Krishnamoorthy P, Stella J, Mohamed AJ, Anand M. Radical scavenging and antibacterial evaluation of Pterocarpussantalinus leaf in-vitro study. Int J Pharm Sci Res. 2011;2:1204–8.

Abdi Z, Eskandary H, Nematollahi-Mahani SN. Effects of Two Types of Human Cells on Outgrowth of Human Glioma in Rats.Turk Neurosurg. 2018;28(1):19-28. [PubMed ID: 27943226]. https://doi.org/107/1019-5149.JTN.18697-16.1.

Joseph OS , Builders M , Joseph O T , Famojuro TI, Ogira JO, Moses FD, Musa TL. Effect of the Demographic of Covid-19 on Different Countries; Using the USA for Comparism.IJMRA. Volume 04 Issue , 2021; 02. Page 193-203.

Joseph SO, Opeyemi JT. Effect of Clinical Study of Moringaoleifera on Body mass index, Low density lipoprotein and Triglyceride level in Patients on Tenofovir/lamivudine/efavirenz Combination Therapy. Adv. Herb. Med. 2021, Vol. 6.Issue 1.Page. 14-27 .

Mishra A, Srivastava R, Strivastava SP, Gautam S, Tamrakar AK, Maurya R. and Strivastava AK.Antidiabetic activity of heart wood of PterocarpusmarsupiumRoxb.and analysis of phytoconstituents. Indian J. Exp. Biol., 2013; 51(5): 363 – 374.

Sheikhbahaei F, Khazaei M, Nematollahi-Mahani SN. Teucriumpolium Extract Enhances the Anti-Angiogenesis Effect of Tranilast on Human Umbilical Vein Endothelial Cells. Adv Pharm Bull. 2018;8(1):131-9. [PubMed ID: 29670848]. [PubMed Central ID: PMC5896388].

Okokon JE., Joseph OS.andUmoh EE.. Nephroprotective activity of Homaliumletestui stem extract against paracetamol induced kidney injury. J ExpIntegr Med2016. Volume 6 (1): 38-43. DOI: 10.5455/jeim.250216.or.147.

Falodun A, Siraj R, Choudhary MI. GC-MS Insecticidal Leaf essential oil of P. staudtii Hutch and Dalz (Icacinaceae). Trop J. Pharm Res. 2009; 82:139-143.

Okolie NP, Falodun A, Oluseyi D. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetiatripetala), and its potential for the inhibition of Lipid peroxidation. Afr J. TradCompl and Altern Med. 2014; 11(3):221-227. Doi: 10.4314/ajtcam. v11i3.31

Simonyan KV, Chavushyan VA. Protective effects of hydroponic Teucriumpolium on hippocampal neurodegeneration in ovariectomized rats. BMC Complement Altern Med. 2016;16(1):415. [PubMed ID: 27776515]. [PubMed Central ID: PMC5078961]. https://doi.org/10.1186/s12906-016-1407-3.

Joseph OS, Builders M, Emem EU and Joseph OT. Effect of ethanol leaf extract of cassia angustifolia extract on liver of wister rats. GSJ.2021; 8(9).1112-11120.

Cheng-Chung WJ, Huang H.C, Chen WJ, Huang CN, Peng CH, and Lin CL. Epigallocatechingallate attenuates amyloid β-induced inflammation and neurotoxicity in EOC 13.31 microglia. Eur. J. Pharmacol. 2016;770, 16–24. doi: 10.1016/j.ejphar.2015.11.048




How to Cite

Oyepata, J. S., Opeyemi, J. T., Adekunle, A. T., & Moses, O. A. (2024). Effect of <i>Pterocarpus santalinus</i> Ethanol Leaf Extract on Haematological, Histopathological and Lipid Profile Indices in Wistar Rats. Tropical Journal of Natural Product Research (TJNPR), 8(3), 6681–6685. https://doi.org/10.26538/tjnpr/v8i3.30