Effectiveness of Black Soldier Fly (Hermetia illucens) Prepupa Oil Emulgel for Burn Wound Recovery
DOI:
https://doi.org/10.26538/tjnpr/v8i3.17Keywords:
wound healing, prepupa, macrophages, fatty acid, EmulgelAbstract
High levels of fatty acids found in black soldier fly (BSF) prepupa oil have the potential to act as antimicrobials and anti-inflammatory agents. This study aimed to evaluate the efficacy of BSF prepupa oil emulgel as a topical treatment to hasten the morphological and histological healing of burn wounds. This study used 30 male BALB/c mice divided into six groups, namely the group without treatment (UT group), the group given emulgel base (EB group), the group given bioplacenton® (Bp group), the group given 5%, 10%, and 15% BSF prepupa oil emulgel (EBSFPO 5%, 10%, and 15% groups). Burns were made by exposing the skin of the back to 25% phenol for 30 seconds; the treatment was carried out for 14 days with application twice a day for all groups. The morphological results showed that the 15% emulgel treatment accelerated the decrease in scab score on day 5 and decreased the wound area faster on day 7 by 0.40 cm2 compared to the other groups. Histological analysis also confirmed that the untreated group had a significant effect
in reducing the thickness of the epidermis. In contrast, emulgel 15% significantly reduced the thickness of the dermis and the number of macrophage cells. Meanwhile, epithelialization and fibroblast formation were complete in all treatment groups after 14 days. The findings suggested that BSF 15% prepupa oil emulgel can be produced as a topical solution that works well for treating wounds.
References
Blanco CER, Rivero CER, Martinez LAD, Avila LMS. Infection in burn patients in a referral center in Colombia. Burns. 2017; 43(3):642-653.
Muthu C, Ayyanar M, Raja N, Ignacimuthu S. Medicinal plants used by traditional healers in Kancheepuram district of Tamil Nadu, India. J Ethnobiol Ethnomed. 2006; 7(2):43.
Rong X, Chu W, Zhang H, Wang Y, Qi X, Zhang G, Wang Y, Li C. Antler stem cell-conditioned medium stimulates regenerative wound healing in rats. Stem Cell Res Ther. 2019; 10(1):326.
Widayati A, Suryawati S, Crespigny C, Hiller JE. Beliefs about the use of nonprescribed antibiotics among people in Yogyakarta City, Indonesia: A qualitative study based on the theory of planned behavior. Asia Pac J Public Health. 2015; 27(2):402-413.
Choi WH, Jiang M. Evaluation of antibacterial activity of hexanedioic acid isolated from Hermetia illucens larvae. J. Appl. Biomed. 2014; 12(3):179-189.
Mumcuoglu KY, Ingber A, Gilead L, Stessman J, Friedmann R, Schulman H, Bichucher H, Ioffe-Uspensky I, Miller J, Galun R, Raz I. Maggot therapy for the treatment of intractable wounds. Int. J. Dermatol. 1999; 38(8):623-627.
Armstrong DG, Mossel J, Short B, Nixon BP, Knowles EA, Boulton AJ. Maggot debridement therapy: a primer. J. Am. Podiatr. Med. Assoc. 2002; 92(7):398-401.
Wang J, Wang S, Zhao G, Wang Z, Lineaweaver WC, Zhang F. Treatment of infected wounds with maggot therapy after replantation. J. Reconstr. Microsurg. 2006; 22(4):277-280.
Gasco L, Finke M, van Huis A. Can Diets Containing Insects Promote Animal Health? J. Insects Food Feed. 2018; 4(1):1-4.
Papada E, Kaliora AC, Gioxari A, Papalois A, Forbes A. Anti-Inflammatory Effect of Elemental Diets with Different Fat Composition in Experimental Colitis. Br. J. Nutr. 2014; 111(7):1213-1220.
Brown TL, Petrovski S, Chan HT, Angove MJ, Tucci J. Semi-Solid and Solid Dosage Forms for the Delivery of Phage Therapy to Epithelia. Pharmaceuticals (Basel). 2018; 11(1):26.
Nofriyanti, Sinata N, Mistawati A. Formulation and Activity Test of Cork Fish Oil Emulgel (Channa striata) as a Burn Healer. Galenika J of Pharmacy. 2020; 6(2):253-268.
Sumoza N, Efrizal, Rahayu R. The Effect of Gambir (Uncaria gambir R.) on Burn Healing in Male White Mice (Mus musculus L.). JBioUA. 2014; 3(4):283-288.
Azzahrah NF, Jamaluddin AW, and Adikurniawan YM. Effectiveness of a Simple Patch from Javanese Wood Leaf Extract (Lannea coromandelica) on Healing Cut Wounds in Mice (Mus musculus L). Pharm J. 2019; 11(2):169-180.
Xu J, Su M, Jin Z, Zhou W, Sun Y, Jin Y, Shi Z. Effects of Natural Brown Cotton Bleached Gauze on Wound Healing. Materials (Basel). 2022; 15(6):2070.
Zayac AS, olszewski AJ. Burkitt lymphoma: bridging the gap between advances in molecular biology and therapy. Leuk Lymphoma. 2020; 61(8):1-13.
Widiyani T, Iriansyah AH, Listyawati S. Biological Activity of catfish (Clarias gariepinus) Mucus Gel on the Wistar (Ratus novergicus) Wound Healing Process. Trop. J. Nat. Prod. Res. 2023; 7(2):2382-2387.
Putra RE, Fatmalasari Y, Kinasih I, Permana AD, Rosmiati M, Riana EN. Omega-3 Content of Black Soldier Fly Prepupa (Hermetia illucens) fed with marine Fish Offal and Tofu Dreg. BIOTROPIA. 2021; 28(1):64-73.
Alexander JW, Supp DM. Role of arginine and omega-3 fatty acids in wound healing and infection. Adv Wound Care (New Rochelle). 2014; 3(11):682-690.
Richter H, Gover O, Schwartz B. Anti-Inflammatory Activity of Black Soldier Fly Oil Associated with Modulation of TLR Signaling: A Metabolomic Approach. Int J Mol Sci. 2023; 24(13):10634.
Dovi JV, Szpaderska AM, DiPietro LA. Neutrofil function in the healing woung:adding insult to injury. Thromb Haemost. 2004; 92(2):275-280.
Choi WH, Yun JH, Chu JP, Chu KB. Antibacterial effect of extracts of Hermetia illucens (Diptera: Stratiomyidae) larvae against Gram-negative bacteria. Entomol. Res. 2012; 42:219–226.
Muller A, Wolf D, Gutzeit HO. The black soldier fly, Hermetia illucens - a promising source for sustainable production of proteins, lipids and bioactive substances. Z Naturforsch C J Biosci. 2017; 72(9-10):351-363.
Caligiani A, Marseglia A, Sorci A, Bonzanini F, Lolli V, Maistrello L, Sforza S. Influence of the killing method of the black soldier fly on its lipid composition. Food Res Int. 2019; 116:276-282.
Jabeen S, Clough ECS, Thomlinson AM, Chadwick SL, Ferguson MWJ, and Shah M. Partial thickness wound: does mechanism of injury influence healing? Burns. 2019; 45(3):531–542.
Rabani V, Cheatsazan H, Davani S. Proteomics and Lipidomics of Black Soldier Fly (Diptera: Stratiomyidae) and Blow Fly (Diptera: Calliphoridae) Larvae. J Insect Sci. 2019; 19(3):29.
Musdja MY, Hapsari MA, Agusta A. Comparison of Activity and Inhibitory Mechanism between (+)-Catechin and Water Extract of Gambier (Uncaria gambir Roxb.) Against Some Bacteria. Journal of PPI-UKM. 2017; 4:55-60.
Rodrigues HG, Vinolo MA, Sato FT, Magdalon J, Kuhl CM, Yamagata AS, Pessoa AF, Malheiros G, Dos Santos MF, Lima C, Farsky SH, Camara NO, Williner MR, Bernal CA, Calder PC, Curi R. Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound
Healing in Diabetic Rats. PLoS One. 2016; 11(10):e0165115.
Naveh HRJ, Taghavi MM, Shariati M, Vazeirnejad R, and Rezvani ME. Both omega-3 and omega-6 polyunsaturated fatty acids stimulate foot wound healing in chronic diabetic rats. Afr J of Pharm and Pharmacol. 2011; 5(14):1713-1717.
Oliveira APD, Franco ES, Rodrigues BR, Cordeiro DP, Melo RGD, Aquino CMFD, Silva AARE, Medeiros PLD, Silva TGD, Goes AJDS, Maia MBDS. Effect of semi-solid formulation of Persea americana mill (avocado) oil on wound healing in rats. Evid Based Complement Alternat
Med. 2013; 2013:472382.
Ladeska V, Dwita LP, Oktaviany F, Putri OF, Salamah Y, Amelia F. Effect of Stelechocarpus burahol [Blume] Leaf Ethanol Extract Oinment on Burns Healing. Trop. J. Nat. Prod. Res. 2022; 6(9):1434-1439.
Bardaa S, Ben HN, Aloui F, Ben MR, Jabeur H, Bouaziz M, Sahnoun Z. Oil from pumpkin (Cucurbita pepo L.) seeds: evaluation of its functional properties on wound healing in rats. Lipids Health Dis. 2016; 15:73.
Nuutila K, Eriksson E. Moist Wound Healing with Commonly Available Dressings. Adv Wound Care (New Rochelle). 2021; 10(12):685-698.
Chiari BG, Trovatti E, Pecoraro É, Correa MA, Cicarelli RMB, Ribeiro SJL, Isaac VLB. Synergistic Effect of Green Coffee Oil and Synthetic Sunscreen for Health Care Application. Ind. Crops Prod. 2014; 52:389-393.
Alasmari KM, Zeid IA, Al-Attar A. Medicinal Properties of Arabica Coffee (Coffea arabica) Oil: An Overview. Adv. life sci. 2020; 8(1):20-29.
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Tropical Journal of Natural Product Research (TJNPR)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
