Beneficial Effects of Self-nanoemulsifying Drug Delivery System Extract of Curcuma longa on Polycystic Ovary Syndrome Rats Model Through Insulin Sensitization Activity

Authors

  • Alfaina Wahyuni Doctoral Program of Medical Science, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia.
  • Ambar Mudigdo Doctoral Program of Medical Science, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia.
  • Soetrisno Doctoral Program of Medical Science, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia.
  • Brian Wasita Doctoral Program of Medical Science, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia.
  • Uki R. Budi Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia
  • Vitri Widyaningsih Public Health and Preventive Medicine, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia
  • Ika P. Sari Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sleman, 55281, Special Region of Yogyakarta, Indonesia

DOI:

https://doi.org/10.26538/tjnpr/v8i3.14

Keywords:

HOMA-IR, Insulin, GLUT4, PCOS, SNEDDS Curcuma longa extract

Abstract

Insulin resistance contributes to the Polycystic Ovary Syndrome (PCOS) pathogenesis. Although Curcuma longa improves insulin sensitivity, it is limited by its low bioavailability. This study aims to demonstrate the application of the Self-Nanoemulsifying Drug Delivery System (SNEDDS) on Curcuma longa extract on the improvement of insulin resistance through measuring Glut-4 expression, fasting insulin levels, fasting blood glucose levels, and Homeostatic Model Assessment-Insulin Resistance (HOMA-IR) of PCOS model rat. This experimental research was conducted with a post-test-only control group design. This study employed 36 female
Wistar rats aged three months divided into six groups: N: normal rats; PCOS: PCOS rats without treatment; PM: PCOS rats with metformin 20mg/kgBW/day; PSC25, PSC50, and PSC100: PCOS rats with SNEDDS extract of Curcuma longa 25, 50 and 100 mg/kgBW/day. Letrozole and a highcholesterol, high-fructose diet was used to induce PCOS in all rats, except the control group, for
21 days before treatment began. Rats were sacrificed on the fifteenth day, and blood samples and gastrocnemius muscle were taken. A statistical test used the Anova and Kruskal-Wallis test with a p-value < 0.05 considered significant. Self-nanoemulsifying Drug Delivery System (SNEDDS) extract of Curcuma longa at doses of 50 and 100 mg/kgBW could significantly increase Glut-4 in muscle cells while decreasing FBG, insulin, and HOMA-IR score (P<0.05). Self-nanoemulsifying Drug Delivery System (SNEDDS) extract of Curcuma longa at 50 and 100 mg/kgBW improved expression of Glut-4, FBG, Insulin level, and HOMA-IR score in PCOS rats via insulin sensitizer activity.

Author Biographies

Alfaina Wahyuni, Doctoral Program of Medical Science, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia.

Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Bantul, 55183, Special Region of Yogyakarta, Indonesia.

Ambar Mudigdo, Doctoral Program of Medical Science, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia.

Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia

Brian Wasita, Doctoral Program of Medical Science, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia.

Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126, Central of Java, Indonesia

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Published

2024-03-30

How to Cite

Wahyuni, A., Mudigdo, A., Soetrisno, Wasita, B., Budi, U. R., Widyaningsih, V., & Sari, I. P. (2024). Beneficial Effects of Self-nanoemulsifying Drug Delivery System Extract of Curcuma longa on Polycystic Ovary Syndrome Rats Model Through Insulin Sensitization Activity. Tropical Journal of Natural Product Research (TJNPR), 8(3), 6563–6569. https://doi.org/10.26538/tjnpr/v8i3.14

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