Evaluation of Polyphenolic Content, Antioxidant and Antimicrobial Activities, and Toxicity Study of <i>Ferula communis</i> L. Fruits


  • Imad Ed-Dahmani Laboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
  • Mohammed Kara Laboratory of Biotechnology, Conservation and Valorisation of Natural Resources (LBCVNR), Faculty of Sciences Dhar El Mehraz, Sidi Mohamed Ben Abdallah University, Fez 30000, Morocco
  • Aziza Lfitat Laboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
  • Hanane Touijer Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar E Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
  • Fatima Z. Bousraf Laboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
  • Meryem Slighoua Laboratory of Biotechnology, Environment, Agri-Food, and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
  • Ghizlane Nouioura Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health and Quality of Life (SNAMOPEQ), Faculty of Sciences Dhar El-Mehraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
  • Yassine El Atki High Institute of Nursing Professions and Health Techniques Errachidia, Morocco
  • Mohamed El Fadili LIMAS Laboratory, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, 30000 Fez, Morocco.
  • Mustapha Taleb Laboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco
  • Abdelfattah Abdellaoui Laboratory of Engineering, Electrochemistry, Modeling and Environment, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez 30000, Morocco




Toxicity, Antimicrobial activity, Antioxidant activity, Phytochemicals, Ferula communis


Many species of the genus Ferula, including Ferula communis are used medicinally to treat various diseases. Consumption of Ferula communis has been reported to have toxic effect. The study aims to evaluate the polyphenolic content, antioxidant, antimicrobial, and toxicological activities of the methanol and aqueous extracts of the edible fruit (MEFE and AEFE) and the fruit at the advanced stage of flowering (AEFAF and MEFAF) of Ferula communis. The polyphenolic (total phenolic and flavonoid) contents were evaluated using standard procedures. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, Ferric Reducing Antioxidant Power (FRAP), and Total Antioxidant Capacity (TAC) assays. The antimicrobial activity was evaluated against selected bacteria and fungal organisms using the disk diffusion method. Acute and sub-acute toxicity of the hydroethanol extract of the edible fruit (HEFE) was evaluated in mice according to standard procedures. The results indicate that the AEFE has higher phenolic and flavonoid content than the MEFE. Whereas, the MEFAF showed a very high amount of phenols and flavonoids compared to AEFAF. AEFAF displayed a higher antioxidant activity in the DPPH and FRAP assays, whereas MEFE showed higher antioxidant activity in the ABTS assay. AEEF and MEEF showed remarkable antibacterial action. Acute and sub-acute oral administration of HEFE did not cause any toxic effect in mice. On the basis of these findings, Ferula communis fruit could serve as a potential antioxidant against oxidative stress, and a potential antibacterial agent against pathogenic bacterial infection.


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How to Cite

Ed-Dahmani, I., Kara, M., Lfitat, A., Touijer, H., Bousraf, F. Z., Slighoua, M., … Abdellaoui, A. (2024). Evaluation of Polyphenolic Content, Antioxidant and Antimicrobial Activities, and Toxicity Study of <i>Ferula communis</i> L. Fruits. Tropical Journal of Natural Product Research (TJNPR), 8(2), 6423–6432. https://doi.org/10.26538/tjnpr/v8i2.34

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