Characterisation and Quality Assessment of Phospholipid from Sesame Seeds (<i>Sesamum indicum</i>)
DOI:
https://doi.org/10.26538/tjnpr/v8i2.22Keywords:
fatty acid, antioxidant activity, peroxide number, phospholipid, SesameAbstract
Phospholipids are amphiphilic molecules that can be used as basic materials for drug delivery systems. Phospholipids can be obtained from natural sources such as sesame seeds. This study investigated the chemical properties of a phospholipid and the total lipids extracted from sesame seeds. The phospholipid extract, with a yield of 0.475%, was brownish-yellow. It has a crude fat content of 96% comprising of saturated fatty acids (Methyl, 14-methyl-Pentadecanoic acid) and two unsaturated fatty acids (9,12-Octadecadienoic acid and 11-Octadecenoic acid). FTIR analysis of the phospholipid revealed the presence of double bonds and a characteristic choline absorption. The antioxidant screening using the DPPH free radical scavenging method showed 34.16% and 30.98 % antioxidant activity on days 7 and 14, respectively. A peroxide number test revealed that it maintained a low peroxide value of 3.1988 mg O2/g even after 14 days in storage. The study concluded that the phospholipid extract of sesame seed has good antioxidant activity, chemical properties, and quality and could be used as a pharmaceutical excipient in drug delivery.
References
Hudiyanti D, Hamidi NI, Anugrah DS, Salimah SN, Siahaan P. Encapsulation of vitamin C in sesame liposomes: computational and experimental studies. Open Chem J. 2019; 17(1): 537-43.
Hudiyanti D, Fawrin H, Siahaan P. Simultant encapsulation of vitamin C and beta-carotene in Sesame (Sesamum indicum L.) liposomes. In IOP conference series: Materials Sci, and Engr. 2018; 349(1): 012014.
Hudiyanti D, Raharjo T J, Noegrohati S. Isolation and Characterization of Plant Lecithin. Agritech 2012; 32(1): 23–26.
Beshaw T, Demssie K, Tefera M, Guadie A. Determination of Proximate Composition, Selected Essential and Heavy Metals in Sesame Seeds (Sesamum Indicum L.) from the Ethiopian Markets and Assessment of the Associated Health Risks. Toxicol. Reports 2022; 9: 1806–1812.
Mi S, Wang Y, Zhang X, Sang Y, Wang X. Authentication of the Geographical Origin of Sesame Seeds Based on Proximate Composition, Multi-Element and Volatile Fingerprinting Combined with Chemometrics. Food Chem. 2022; 397: 2596.
AOCS. Off. Method Ca 2e-84. AOCS. 2005
AOCS. Off. Method Cd 8b–90. AOCS. 2009.
Zhang N, Li Y, Wen S, Sun Y, Chen J, Gao Y, Sagymbek A, Yu X. Analytical Methods for Determining the Peroxide Value of Edible Oils: A Mini-Review. Food Chem. 2021; 358: 129834.
Dravie EE, Kortei NK, Essuman EK, Tettey CO, Boakye AA, Hunkpe G. Antioxidant, Phytochemical and Physicochemical Properties of Sesame Seed
(Sesamum Indicum L). Sci. African 2020; 8: e00349.
Agidew MG, Dubale AA, Atlabachew M, Abebe W. Fatty acid composition, total phenolic contents and antioxidant activity of white and black sesame seed varieties from different localities of Ethiopia. Chem Biol Technol Agric. 2021; 8(1): 1–10.
Putri D N, Hidayat C, Hastuti P. Phospholipid Fractionation and Characterisation of Calophylluminophyllum Kernel. J. Teknol. Pangan 2018; 12(1): 12–17.
Hudiyanti D. Fosfolipida: Biosurfaktan. Yogyakarta: Deepublish; 2018. 79 p.
SNI. No. 01-3176-1992 Biji Wijen. SNI. 1992.
SNI. No. 01-4457-1998 Lesitin. SNI. 1998.
Hudiyanti D, Jayanti M, Al-Khafiz MF, Anam K. A study of coconut (Cocos nucifera L.) phosphatidylcholine species. Orient J Chem. 2018; 34(6): 2963–2968.
Guo A, Li S, Yang Y, Hou F, Wu J, Gao Y, Xing G. Lecithin extraction optimisation and synthesis in Hemerocallis citrina Baroni. Sci Hortic (Amsterdam). 2022; 293: 110682.
Arifan F, Nandita MK, Sapatra EF, Salsabila S. Utilization of Corn Oil through Water Degumming Process for Lecithin Emulsifier Production. Indones J Chem. 2021; 21(6): 1408–1417.
Aparamarta HW, Qadariyah L, Gunawan S, Ju YH. Separation and identification of fatty acid in triacylglycerol isolated from Calophyllumino phyllum oil. ARPN J Eng Appl Sci 2018; 13(2): 442–451.
Nurdin M, Fatma F, Natsir M, Wibowo D. Characterization of methyl ester compound of biodiesel from industrial liquid waste of crude palm oil processing. Anal Chem Res. 2017; 12: 1–9.
Bharathwaaj R, Nagarajan PK, Kabeel AE, Madhu B, Mageshbabu D, Sathyamurthy R. Formation, characterization and theoretical evaluation of combustion of biodiesel obtained from wax esters of A. Mellifera. Alexandria Eng J. 2018; 57(3): 1205–1215.
Sugino H, Ishikawa M, Nitoda T, Koketsu M, Juneja LR, Kim M, Yamamoto T. Antioxidative activity of egg yolk phospholipids. J Agric Food Chem. 1997: 45(3): 551–554.
Li J, Guo Z. Identification and Quantification of Phenolic Compounds in Rapeseed Originated Lecithin and Antioxidant Activity Evaluation. LWT - Food Sci. Technol. 2016; 73: 397–405.
Fessenden RJ, Fessenden J. Kimia Organik. Jakarta: Erlangga; 1982. 592 p.
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