Evaluation of In Vitro Antioxidant Activities and Toxicity Effects of a Novel Plant-Based Body Lotion from Thai Medicinal Plants
Article Sidebar
Main Article Content
Abstract
In addition to lessening the residue of chemicals, which are the primary ingredients in cosmetics, the hunt for herbal extracts with skin health benefits is currently highly intriguing. Local herbs are more valued as a result. This study presents the development and evaluation of a novel plant-based body lotion formulated with extracts from Thai medicinal plants, including Stemona tuberosa. The research aimed to assess the antioxidant activities, toxicity effects, and sensory characteristics of the lotion. Antioxidant properties were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic content (TPC), and ferric-reducing antioxidant power (FRAP) assays, revealing significant antioxidant capabilities, particularly for the Stemona tuberosa extract. Toxicity was evaluated using Galleria mellonella larvae, demonstrating minimal adverse effects in a lotion containing various concentrations of the extracts. The most promising lotion's formulation incorporated different concentrations of Stemona tuberosa extract (1, 3, and 5% w/w), with other ingredients including stearic acid, isopropyl myristate, and glyceryl monostearate, ensuring stability and homogeneity. Sensory evaluations were conducted employing a semi-trained panel of 40 judges using a 5-point hedonic scale, focusing on characteristics such as color, fragrance, texture, and overall acceptability. The lotion displayed favorable sensory properties, with different concentrations yielding slightly varied consumer preferences. Overall, the results indicate that a novel herbal lotion formulated with Stemona tuberosa is a promising candidate skincare product, offering antioxidant benefits and consumer acceptability while maintaining low toxicity.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Chokpaisarn J, Yincharoen K, Sanpinit S, Karutha Pandian ST, Nandhini JR, Gowrishankar S, Limsuwan S, Kunworarath N, Voravuthikunchai SP, Chusri S. Effects of a traditional Thai polyherbal medicine ‘Ya-Samarn-Phlae’ as a natural anti-biofilm agent against Pseudomonas aeruginosa. Microb Pathog. 2019; 128:354-362. Doi: 10.1016/j.micpath. 2019.01.036
Chumpolphant S, Suwatronnakorn M, Issaravanich S, Tencomnao T, Prasansuklab A. Polyherbal formulation exerts wound healing, anti-inflammatory, angiogenic and antimicrobial properties: Potential role in the treatment of diabetic foot ulcers. Saudi J Biol Sci. 2022; 29(7):103330. Doi: 10.1016/j.sjbs.2022.103330
Lohakul J, Chaiprasongsuk A, Jeayeng S, Saelim M, Muanjumpon P, Thanachaiphiwat S. Panich U. The protective effect of polyherbal formulation, harak formula, on uva-induced photoaging of human dermal fibroblasts and mouse skin via promoting nrf2-regulated antioxidant defense. Frontiers in pharmacology. 2021; 12: 649820. Doi: org/10.3389/fphar.2021.649820
Shen T, Xing G, Zhu J, Cai Y, Zhang S, Xu G, Feng Y, Li D, Rao J, Shi R. Effects of 12-week supplementation of a polyherbal formulation in old adults with prehypertension/hypertension: a randomized, double-blind, placebo-controlled trial. Evid Based Complement Alternat Med. 2019; 2019:7056872. Doi.org/10.1155/2019/7056872
Dubey S, Dixit AK. Preclinical evidence of polyherbal formulations on wound healing: A systematic review on research trends and perspectives. J Ayurveda Integr Med. 2023; 14(2):100688. Doi: 10.1016/j.jaim.2023.100688
Srivastava A, Rengaraju M, Srivastava S, Narayanan V, Gupta V, Upadhayay R, Kumar J, Parameswaran S, Kadarkarai K, Velmurugan A. Efficacy of two siddha polyherbal decoctions, Nilavembu Kudineer and Kaba Sura Kudineer, along with standard allopathy treatment in the management of mild to moderate symptomatic COVID-19 patients-a double-blind, placebo-controlled, clinical trial. Trials. 2021; 22(1):570. Doi: 10.1186/s13063-021-05478-0
Siddique YH. Role of Unani polyherbal formulations in the treatment of diseases with special reference to neurodegenerative disorders. CNS Neurol Disord Drug Targets. 2023; 22(3):321-328. Doi: 10.2174/1871527321666220127141611
Van Vuuren SF, Motlhatlego KE, Netshia V. Traditionally used polyherbals in a southern African therapeutic context. J Ethnopharmacol. 2022; 288:114977. Doi: 10.1016/j.jep.2022.114977
Karole S, Shrivastava S, Thomas S, Soni B, Khan SU, Dubey J, Dubey SP, Khan N, Jain DK. Polyherbal formulation concept for synergic action: a review. J Drug Deliv Ther. 2019; 9(1-s):449-452. Doi:10.22270/jddt.v9i1-s.2339
Saising J, Maneenoon K, Sakulkeo O, Limsuwan S, Gotz F, Voravuthikunchai S P. Ethnomedicinal Plants in Herbal Remedies Used for Treatment of Skin Diseases by Traditional Healers in Songkhla Province, Thailand. Plants. 2022; 11(7): 880. Doi: org/10.3390/plants11070880
Liu Y, Shen Y, Teng L, Yang L, Cao K, Fu Q, Zhang J. The traditional uses, phytochemistry, and pharmacology of Stemona species: A review. J Ethnopharmacol. 2021; 265:113112. Doi: 10.1016/j.jep.2020.113112
Loahaprapanon S, Yincharoen K, Nukong J, Chanwun T. An Investigation on the Antibacterial and Antibiofilm Efficacy of a Traditional Thai Herbal Recipe (THR 01) against Clinical Isolates of Methicillin Resistant Staphylococcus Aureus (MRSA) and Staphylococcus epidermidis. International Journal of Agricultural Technology. 2018; 14(3): 325-332.
Sakulpanich A, Attrapadung S, Gritsanapan W. Larvicidal activity of Stemona collinsiae root extract against Musca domestica and Chrysomya megacephala. Sci Rep. 2023; 13(1):15689. Doi.org/10.1038/s41598-023-42500-8
Xu Y, Xiong L, Yan Y, Sun D, Duan Y, Li H, Chen L. Alkaloids from Stemona tuberosa and their anti-inflammatory activity. Frontiers in Chemistry. 2022; 10:847595. Doi.org/10.3389/fchem.2022.847595
Ruangchuay S, Wang QQ, Wang LY, Lin J, Wang YC, Zhong GH, Maneenoon K, Huang ZB, Chusri S. Antioxidant and antiaging effect of traditional Thai rejuvenation medicines in Caenorhabditis elegans. J Integr Med. 2021; 19(4):362-373. Doi.org/10.1016/j.joim.2021.03.004
Siriyong T, Voravuthikunchai SP, Coote PJ. Steroidal alkaloids and conessine from the medicinal plant Holarrhena antidysenterica restore antibiotic efficacy in a Galleria mellonella model of multidrug-resistant Pseudomonas aeruginosa infection. BMC Complementary and Altern Med. 2018; 18(1):1-10. Doi.org/10.1186/s.12906-018-2348-9
Mbarga MJA, Podoprigora IV, Anyutoulou K LD. Galleria mellonella (greater wax moth) as an eco-friendly in vivo approach for the assessment of the acute toxicity of medicinal plants: Application to some plants from Cameroon. Open Vet. J. 2021; 11(4): 651-661. Doi: org/10.5455/OVJ.2021.v11.i4.15
Ilomuanya M O, Ekerebe Z, Cardoso-Daodu I, Sowemimo A. Formulation and Evaluation of Sunscreen Cream Using Detarium senegalenseOil as Base. Trop J Nat Prod Res. 2020; 4(4):141-145. Doi.org/10.26538/tjnpr/v4i4.5
Rasidah R, Maurizka I S, Aulianshah V, Munira M, Zakiah N, Handayani R. Repellent Activity of Lotion of Essential Oils from Piper betle Linn and Cymbopogon nardus [L.] Trop J Nat Prod Res. 2022; 6(12):2020-2024. Doi.org/10.26538/tjnpr/v6i12.20
Belmes NJ. BATUAN (Garciniabinucao) fruit preservation. In Journal of Physics: Conference Series. 2019; 1254(1): 012030. Doi: 10.1088/1742-6596/1254/1/012030
Tan PL, Rajagopal M, Chinnappan S, Selvaraja M, Leong MY, Tan LF, Yap VL. Formulation and physicochemical evaluation of green cosmeceutical herbal face cream containing standardized mangosteen peel extract. Cosmetics. 2022; 9(3):46. Doi: org/10.3390/cosmetics9030046
Konsue A, Taepongsorat L. Phytochemical Screening and Antioxidant Activity of Longevity Remedy from National Thai Traditional Medicine Scripture (Formulary Special Edition). Trop J of Nat Prod Res. 2022; 6(6). Doi.org/10.26538/tjnpr/v1i4.5
Chen J, Liu Y, Zhao Z, Qiu J. Oxidative stress in the skin: Impact and related protection. Int J Cosmet Sci. 2021; 43(5):495-509. Doi: 10.1111/ics.12728
Azevedo Martins TE, Sales de Oliveira Pinto C A, Costa de Oliveira A, Robles Velasco MV, Gorriti Guitierrez AR, Cosquillo Rafael M F, Retuerto-Figueroa MG. Contribution of topical antioxidants to maintain healthy skin-A review. Scientia Pharmaceutica. 2020; 88(2): 27. Doi.org/10.3390/scipharm88020027
Michalak M. Plant-derived antioxidants: Significance in skin health and the ageing process. International journal of molecular sciences. 2022; 23(2): 585. Doi: org/10.3390/ijms23020585
Kim E, Hwang K, Lee J, Han SY, Kim EM, Park J, Cho JY. Skin protective effect of epigallocatechin gallate. Int J Mol Sci. 2018; 19(1):173. Doi: 10.3390/ijms19010173
Cerulli A, Masullo M, Montoro P, Piacente S. Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and their constituents as active cosmeceutical ingredients. Cosmetics. 2022; 9(1): 7. Doi: org/10.3390/cosmetics9010007
Henning SM, Yang J, Lee RP, Huang J, Hsu M, Thames G, Gilbuena I, Long J, Xu Y, Park EH, Tseng CH, Kim J, Heber D, Li Z. Pomegranate juice and extract consumption increases the resistance to uvb-induced erythema and changes the skin microbiome in healthy women: a randomized controlled trial. Sci Rep. 2019; 9(1):14528. Doi: 10.1038/s41598-019-50926-2
Sah A, Naseef PP, Kuruniyan MS, Jain GK, Zakir F, Aggarwal G. A Comprehensive study of therapeutic applications of chamomile. Pharmaceuticals (Basel). 2022; 15(10):1284. Doi: 10.3390/ph15101284
Kumar B, Aggarwal R, Prakash U, Sahoo PK. Emerging therapeutic potential of curcumin in the management of dermatological diseases: an extensive review of drug and pharmacological activities. Future J Pharm Sci. 2023; 9(1):42. Doi.org/10.1186/s43094-023-00493-1
Michalak M. Plant-derived antioxidants: Significance in skin health and the ageing process. Int J Mol Sci. 2022; 23(2):585. Doi: 10.3390/ijms23020585
Liu Y, Shen Y, Teng L, Yang L, Cao K, Fu Q, Zhang J. The traditional uses, phytochemistry, and pharmacology of Stemona species: A review. Journal of Ethnopharmacology.2021; 265: 113112. Doi: org/10.1016/j.jep.2020.113112
Greger H. Structural classification and biological activities of Stemona alkaloids. Phytochemistry Reviews. 2019; 18(2): 463-493. Doi: org/10.1007/s11101-019-09602-6
Liu Y, Shen Y, Teng L, Yang L, Cao K, Fu Q, Zhang J. The traditional uses, phytochemistry, and pharmacology of Stemona species: A review. J Ethnopharmacol. 2021; 265:113112. Doi: 10.1016/j.jep.2020.113112
Priyadarsini SS. Formulation and evaluation of a herbal antibacterial cream from ethyl acetate extract of leaves of Spinacia oleracea Linn. against Aeromonas skin and soft tissue infections. Int J Green Pharm. 2018; 12(3):S537-S542. Doi.org/10.22377/ijgp.v12i03.2015
Gupta N, Dubey A, Prasad P, Roy A. Formulation and evaluation of herbal fairness cream comprising hydroalcoholic extracts of Pleurotus ostreatus, Glycyrrhiza glabra and Camellia sinensis. J Pharm Biosci. 2015; 3(3):40-45. Doi:10.20510/ukjpb/3/i3/89410
Soto ML, Parada M, Falqué E, Domínguez H. Personal-care products formulated with natural antioxidant extracts. Cosmetics. 2018; 5(1):13. Doi.org/10.3390/cosmetics5010013
Rodrigues F, Sarmento B, Amaral MH, Oliveira MBPP. Exploring the antioxidant potentiality of two food by-products into a topical cream: stability, in vitro and in vivo evaluation. Drug Dev Ind Pharm. 2016; 42(6):880-889. Doi: 10.3109/03639045.2015.1088865
Widiputri D I, Wijaya S, Kusumocahyo SP. Development of skin lotion containing antioxidant extract from coffee pulp and study on its stability. In IOP Conference Series: Materials Science and Engineering.2020; 742(1): 012020. Doi: 10.1088/1757-899X/742/1/012020
Kusumastuti A, Rahma HS. Application of parijoto (Medinilla speciosa l.,) extract as body lotion. In IOP Conference Series: Earth and Environ Sci. 2021; 810(1): 012054. Doi: 10.1088/1755-1315/810/1/012054.