GC-MS Characterization, Antioxidant, Antimicrobial and Insecticidal Potential of Moroccan Cuminum Cyminum L. Essential Oil
Article Sidebar
Main Article Content
Abstract
Human health has constantly been improved using medicinal plants. Notwithstanding synthetic chemistry advancements, medicinal plants remain widely used due to their effectiveness, reduced toxicity, and therapeutic use. This investigation examines the antioxidant, antimicrobial, and insecticidal properties of essential oil extracted from Moroccan cumin seeds (Cuminum cyminum L.). The essential oil was obtained through hydrodistillation from cumin seeds with a 3% yield. The EO was subjected to antioxidant, antimicrobial, and insecticidal activity screening using standard methods. The EO was investigated against a panel of pathogenic microorganisms using the disc diffusion method. The GC-MS analysis of the EO revealed the presence of 41 constituents. The major compounds identified were cuminaldehyde (21.94%), γ-terpene (18.20%), mentha-1,3-diene-7-al (14.93%), and α-pinene (13.789%). The EO showed moderate antioxidant activity against DDPH free radicals with anIC50 values of 273.409 mg/mL. The antibacterial screening results revealed that the EO of Cuminum cyminum possesses potent antibacterial activity against all tested bacterial strains, with MIC of 0.5 µL/mL against Acinetobacter baumannii and 1 µL/mL against Proteus mirabilis. The EO exhibited potent insecticidal activity against black bean aphids (Aphis fabae Scop.), with an 80% mortality rate. In conclusion, this study shows that Cuminum cyminum L. has interesting pharmacological effects and could be used to develop insecticidal and antimicrobial drug leads.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
How to Cite
References
Jaber H, Oubihi A, Ouryemchi I, Boulamtat R, Oubayoucef A, Bourkhiss B, Ouhssine M. Chemical composition and antibacterial activities of eight plant essential oils from Morocco against Escherichia coli strains isolated from different Turkey organs. Biochem Res Int. 2021;2021:1‑9.
Tarfaoui K, Brhadda N, Ziri R, Oubihi A, Imtara H, Haida S, Al Kamaly OM, Saleh A, Parvez MK, Fettach S, Ouhssine M. Chemical Profile, Antibacterial and Antioxidant Potential of Zingiber officinale Roscoe and Elettaria cardamomum (L.) Maton Essential Oils and Extracts. Plants (Basel). 2022;11(11):1487
Islam ME, Islam KMD, Billah MM, Biswas R, Sohrab MH, Rahman SMM. Antioxidant and anti-inflammatory activity of Heritiera fomes (Buch.-Ham), a mangrove plant of the Sundarbans. Adv Tradit Med. 2020;20:189‑97.
Yahiaoui F, Zekkour N, Boukhalfoun L. Evaluation of the antimicrobial activity of methanolic and aqueous extracts of Eucalyptus sp [Internet] [PhD Thesis]. 2018 [cité 11 déc 2023]. Disponible sur: http://dspace.univmedea.dz/handle/123456789/9161
Özkara A, Akyıl D, Konuk M. Pesticides, environmental pollution, and health. In: Environmental health riskhazardous factors to living species. IntechOpen; 2016.
Smith P, Calvin K, Nkem J, Campbell D, Cherubini F, Grassi G, Korotkov V, Le Hoang A, Lwasa S, McElwee P, Nkonya E. Which practices co‐deliver food security, climate change mitigation and adaptation, and combat land degradation and desertification?. GlobChange Biol. 2020;26(3):1532-75.
Oubihi A, Hosni H, Nounah I, Ettouil A, Harhar H, Alaoui K, Ouhssine M, Guessous Z. Phenolic content, antioxidant activity, anti-inflammatory potential, and acute toxicity study of Thymus leptobotrys Murb. extracts. Biochem Res Int. 2020; DOI: 10.1155/2020/8823209
Hayani M, Bencheikh N, Ailli A, Bouhrim M, Elbouzidi A, Ouassou H, Kharchoufa L, Baraich A, Atbir A, Ayyad F, Drioiche A, Addi A, Hano C and Zair T. Quality Control, Phytochemical Profile, and Antibacterial Effect of Origanum compactum Benth. Essential Oil from Morocco. Int J Plant Biol. 2022;13:546‑60.
Bencheikh N, Elachouri M, Subhash C. M, Noureddine B, Mostafa E, Mandal SC. Ethnobotanical, pharmacological, phytochemical, and clinical investigations on Moroccan medicinal plants traditionally used for the management of renal dysfunctions. J Ethnopharmacol. 2022;292:115178.
Ouassou H, Bouhrim M, Kharchoufa L, Imtara H, Elhouda Daoudi N, Benoutman A, Bencheikh N, Ouahhoud S, Elbouzidi A, Bnouham M. Caralluma europaea (Guss) NE Br.: A review on ethnomedicinal uses, phytochemistry, pharmacological activities, and toxicology. J Ethnopharmacol. 2021;273:113769.
Elbouzidi A, Ouassou H, Aherkou M, Kharchoufa L, Meskali N, Baraich A, Mechchate H, Bouhrim M, Idir A, Hano C, Zair T and Addi M LC–MS/MS Phytochemical Profiling, Antioxidant Activity, and Cytotoxicity of the Ethanolic Extract of Atriplex halimus L. against Breast Cancer Cell Lines: Computational Studies and Experimental Validation. Pharmaceuticals. 2022;15:1‑25.
El Amri J, Elbadaoui K, Zair T, Bouharb H, Chakir S, Alaoui TI. Study of the antibacterial activity of the essential oils of Teucrium capitatium L and the extract of Silene vulgaris on different strains tested. J Appl Biosci. 2014;82:7481‑92.
Yaacoube R, Tlidjane I. Physicochemical characterization and biological analyses of the essential oil of Cuminum cyminum L. and Foeniculum vulgare Mill grains. Extracted by hydrodistillation and Supercritical CO2: Comparative study. 2018. Thesis of Master's Degree in Chemical Engineering, Larbi ben M'Hidi University, Oum el Bouaghi, pp: 98.
El-Sayed HS, El-Sayed SM. A modern trend to preserve white soft cheese using nano-emulsified solutions containing cumin essential oil. Environ Nanotechnol Monit 2021;16:100499.
Mandal M, Mandal S. Cumin (Cuminum cyminum L.) oils. In: essential oils in food preservation, flavor, and safety. Elsevier; 2016; 377‑83.
Bencheikh N, Elbouzidi A, Kharchoufa L, Ouassou H, Alami Merrouni I, Mechchate H, Es-safi I, Hano C, Addi M, Bouhrim M, Eto B, Elachouri M Inventory of medicinal plants used traditionally to manage kidney diseases in northeastern Morocco: Ethnobotanical fieldwork and pharmacological evidence. Plants. 2021;10:1966.
Sharif MK, Ejaz R, Pasha I. Nutritional and therapeutic potential of spices. In: Therapeutic, probiotic, and unconventional foods. Elsevier; 2018; 181‑99.
Srinivasan K. Cumin (Cuminum cyminum) and black cumin (Nigella sativa) seeds: traditional uses, chemical constituents, and nutraceutical effects. Food Qual and Saf. 2018;2:1‑16.
Elyemni M, Louaste B, Nechad I, Elkamli T, Bouia A, Taleb M, Chaouch M and Eloutassi N. Extraction of Essential Oils of Rosmarinus officinalis L. by Two Different Methods: Hydrodistillation and Microwave Assisted Hydrodistillation. Sci World J. DOI:10.1155/2019/3659432
Ouahabi S, Loukili EH, Elbouzidi A, Taibi M, Bouslamti M, Nafidi HA, Salamatullah A , Saidi N, Bellaouchi R, Addi M, Ramdani M, Bourhia M and Hammouti B. Pharmacological Properties of Chemically Characterized Extracts from Mastic Tree: In Vitro and In Silico Assays. Life. 2023;13:1393.
Haida S, Kribii A. Chemical composition, phenolic content and antioxidant capacity of Haloxylon scoparium extracts. S Afr J Bot. 2020;131:151‑60.
Zrouri H, Elbouzidi A, Bouhrim M, Bencheikh N, Kharchoufa L, Ouahhoud S, Ouassou H, El Assri S and Choukri M. Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous tract. Mediterr J Chem. 2021;11:84.
Elbouzidi A, Taibi M, Ouassou H, Ouahhoud S, Ou-Yahia D, Loukili EH, Aherkou M, Mansouri F, Bencheikh N , Laaraj S,, Bellaouchi R, Saalaoui E , Elfazazi K , Berrichi A, Abid M and Addi M. Exploring the Multi-Faceted Potential of Carob (Ceratonia siliqua var. Rahma) Leaves from Morocco: A Comprehensive Analysis of Polyphenols Profile, Antimicrobial Activity, Cytotoxicity against Breast Cancer Cell Lines, and Genotoxicity. Pharmaceuticals. 2023;16:840.
Taibi M, Elbouzidi A, Ou-Yahia D, Dalli M, Bellaouchi R, Tikent A, Roubi M, Gseyra N, Asehraou A Hano C, Addi M , El Guerrouj B and Chaabane K. Assessment of the Antioxidant and Antimicrobial Potential of Ptychotis verticillata Duby Essential Oil from Eastern Morocco: An In Vitro and In Silico Analysis. Antibiotics. 2023;12.
Kandsi F, Elbouzidi A, Lafdil FZ, Meskali N, Azghar A, Addi M, et al. Antibacterial and Antioxidant Activity of Dysphania ambrosioides (L.) Mosyakin and Clemants Essential Oils: Experimental and Computational Approaches. Antibiotics. 2022;11:482.
Oubihi A, Ouryemchi I, Nounah I, Tarfaoui K, Harhar H, Ouhssine M, Guessous Z. Chemical composition, antibacterial and antifungal activities of Thymus leptobotrys Murb essential oil. Adv Tradit Med. 2020;20:673‑9.
Mrabti HN, Hachlafi N El, Al-Mijalli SH, Jeddi M, Elbouzidi A, Abdallah EM, Flouchi R, Assaggaf H , Qasem A, Zengin G, Bouyahya A and Ouazzani Chahdi F Phytochemical profile, assessment of antimicrobial and antioxidant properties of essential oils of Artemisia herbaalba Asso., and Artemisia dracunculus L.: Experimental and computational approaches. J Mol Struct. 2023;1294:136479.
Al-Mijalli SH, Mrabti HN, El Hachlafi N, El Kamili T, Elbouzidi A, Abdallah EM, Flouchi R , Assaggaf H , Qasem A, Zengin G, Bouyahya A and Ouazzani Chahdi Integrated analysis of antimicrobial, antioxidant, and phytochemical properties of Cinnamomum verum: A comprehensive In vitro and In silico study. Biochem Syst Ecol. 2023;110:104700.
Oubihi A, Ouryemchi I, Nounah I, Tarfaoui K, Harhar H, Ouhssine M, Guessous Z. Chemical composition, antibacterial and antifungal activities of Thymus leptobotrys Murb essential oil. Advances in Traditional Medicine. 2020;1‑7.
Rashedi A, Rajabpour A, Sohani NZ, Rasekh A. Prey stage preference and functional response of Orius albidipennis (Hetetroptera, Anthocoridae) to Aphis fabae (Homomoptera, Aphididae). Int J Trop Insect Sci. 2020;40:13‑9.
Alghamdi AS. Insecticidal effect of four plant essential oils against two aphid species under laboratory conditions. J Appl Biol. 2018;6:27‑30.
Khandekar C, Yadu YK, Mohanan A. Efficacy of microbial insecticides and plant product against tobacco caterpillar (Spodoptera litura) on soybean in laboratory conditions. J. Pharm. Innov. 2023; 12(3): 4737-4740
Petretto GL, Fancello F, Bakhy K, Faiz CAL, Sibawayh Z, Chessa M, Zara S, Sanna M.L, Maldini M, Rourke J.P and Pintore G. Chemical composition and antimicrobial activity of essential oils from Cuminum cyminum L. collected in different areas of Morocco. Food Bioscience. 2018;22:50‑8.
Patil SD, Maknikar PP, Wankhade SJ, Ukesh CS, Rai MK. Chemical composition, antimicrobial and antioxidant activity of essential oils from Cumin and Ajowan. Nusantara Bioscience. 2016;8.
Miri Y Ben, Djenane D. Antifungal, anti-aflatoxigenic, antioxidant activity and in vivo efficacy of essential oil of the aerial parts of Thymus capitatus (L.) Hoffmanns & Link. Phytotherapie. 2019;17:299‑309.
da Silva Rivas AC, Lopes PM, de Azevedo Barros MM, Costa Machado DC, Alviano CS, Alviano DS. Biological activities of α-pinene and β-pinene enantiomers. Molecules. 2012 May 25;17(6):6305-16.
Baldissera MD, Grando TH, Souza CF, Gressler LT, Stefani LM, da Silva AS and Monteiro S. In vitro and in vivo action of terpinen-4-ol, γ-terpinene, and α-terpinene against Trypanosoma evansi. Exp Parasitol 2016;162:43‑8.
Khoudali S, Essaqui A, Zertoubi M, Azzi M, Benaissa M. Study of antioxidant activity and anticorrosion action of the methanol extract of dwarf palm leaves (Chamaerops humilis l.) from Morocco J Mater Environ Sci. 5 (3) (2014) 887-898
Belkacem M, Kessar I, EttahrI A. Study of the antifungal and antioxidant effect of the essential oil of Cuminum cyminum L. seeds 2022;
Hajib A, Nounah I, Oubihi A, Harhar H, Gharby S, Kartah B, Bougrin K and Charrouf Z Chemical Composition and Biological Activities of Essential Oils from the Fruits of Cuminum cyminum L. and Ammodaucus leucotrichus L. (Apiaceae). J Essent Oil-Bear Plants. 2020;23:474‑83.
Yaacoube R, Tlidjane I. Yaacoube R, Tlidjane I. Physicochemical characterization and biological analyses of the essential oil of Cuminum cyminum L. and Foeniculum vulgare Mill grains. Extracted by hydrodistillation and Supercritical CO2: Comparative study. 2018. Thesis of Master's Degree in Chemical Engineering, Larbi ben M'Hidi University, Oum el Bouaghi, pp: 98.
Chen Q, Gan Z, Zhao J, Wang Y, Zhang S, Li J, and Ni Y.In vitro comparison of antioxidant capacity of cumin (Cuminum cyminum L.) oils and their main components. LWT-Food Sci and Technol. 2014;55:632‑7.
Hajib A, Nounah I, Oubihi A, Harhar H, Gharby S, Kartah B. Bougrin K and Charrouf Z. Chemical composition andbiological activities of essential oils from the fruits of Cuminum cyminum L. and Ammodaucus leucotrichus L.(Apiaceae). J Essent Oil-Bear Plants. 2020;23:474‑83.
Aouina A and Khelifi NH. Evaluation of the Repellent Effect of Cuminum Cyminum L. Et Foeniculum Vulgare Mill. On the stored grain insect Tribolium Castaneum (herbst). 2017.
Tsitlakidou P, Papachristoforou A, Tasopoulos N, Matzara A, Hatzikamari M, Karamanoli K, and Mourtzinos. Sensory analysis, volatile profiles and antimicrobial properties of Origanum vulgare L. essential oils. Flavour Fragr. J.2022;37:43‑51.
Pinto L, Bonifacio MA, De Giglio E, Cometa S, Logrieco AF, Baruzzi F. Unravelling the antifungal effect of red thyme oil (Thymus vulgaris L.) compounds in vapor phase. Molecules. 2020;25:4761.
Gold K, Slay B, Knackstedt M, Gaharwar AK. Antimicrobial Activity of Metal and Metal‐Oxide Based Nanoparticles. Adv Ther. 2018;1:1700033. DOI:10.1002/adtp.201700033
Moumni F, Djalt Houari Si. L’ Use of Rosmarinus officinalis L against the black aphid (Aphis Fabae) of sugar beet (Beta Vulgaris). Université d’Ain Témouchent Belhadj Bouchaib -UATBB; 2022.
Achimón F, Leal LE, Pizzolitto RP, Brito VD, Alarcón R, Omarini AB, and Zygadlo JA. Insecticidal and antifungal effects of lemon, orange, and grapefruit peel essential oils from Argentina. AgriScientia. 2022;39:71‑82.
Nenaah GE, Almadiy AA, Al‐Assiuty BA, Mahnashi MH. The essential oil of Schinus terebinthifolius and its nanoemulsion and isolated monoterpenes: investigation of their activity against Culex pipiens with insights into the adverse effects on non‐target organisms. Pest Manag Sci. 2022;78:1035‑47.
Sosa-Gómez DR, Corrêa-Ferreira BS, Kraemer B, Pasini A, Husch PE, Delfino Vieira CE, Reis Martinez CB, and Negrão Lopes IO. Prevalence, damage, management and insecticide resistance of stink bug populations (Hemiptera: Pentatomidae) in commodity crops. Agric For Entomol. 2020;22:99‑118.