Chemical Composition, Antioxidant and Antimicrobial Activities of the Essential Oil of Origanum majorana Growing in Middle Atlas of Morocco http://www.doi.org/10.26538/tjnpr/v7i10.16
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Abstract
The purpose of this work was to determine the chemical composition and assess the antibacterial and antioxidant activity of the essential oil of Origanum majorana grown in the Middle Atlas of Morocco. The yield of essential oil extracted by hydro-distillation is about 1 ± 0.05%.The chemical composition of the essential oil extracted from the Aerial parts of Origanum majorana was studied by gas chromatography coupled with mass spectrometry (GC/MS). The results of the GC-MS analysis of the volatile components of the EO revealed the presence of 21 components, representing 98.51% of the total essential oils (EOs). The main compounds were carvacrol (41.09%), terpinen-4-ol (28.08%), followed by p- Cis-Sabinene hydrate (21.12%), γ-Terpinene (2.7%), Spathulenol (1.5%) and thymol (1.32%). Antioxidant activity was determined by the DPPH and FRAP assays. The results demonstrated that our EOs exhibit a significant antiradical effect with an IC50 value of 0.25 mg/mL compared to the pure reference antioxidant BHT with an IC50 value of 0.11 mg/mL. The antibacterial effect of this essential oil was tested against five bacterial strains.Staphylococcus aureus was the most sensitive with a MIC value of 1.98 ± 0.01, followed by Klebsiella pneumonia with an MIC value of 2.01 ± 0.35ug/ml. The results show that Origanum majorana essential oil process antibacterial and antioxidant properties and it can be used as a natural food preservative and as an antimicrobial agent for the treatment of several infectious diseases caused by bacteria.
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Postu PA, Gorgan DL, Cioanca O, Russ M, Mikkat S, Glocker MO, Hritcu L. Memory-Enhancing Effects of Origanum majorana Essential Oil in an Alzheimer’s Amyloid beta1-42 Rat Model: A Molecular and Behavioral Study. Antioxidants. 2020; 9(10):919.
Bina F, Rahimi R. Sweet Marjoram. J Evid Based Complementary Altern Med. 2016; 1(22): 175-185.
Prerna , Vasudeva N. Origanum majorana L. Phyto-pharmacological review. Indian J Nat Prod Resour. 2015; 6: 261-267.
El-Ashmawy IM, el-Nahas AF, Salama OM. Protective effect of volatile oil, alcoholic and aqueous extracts of Origanum majorana on lead acetate toxicity in mice. Basic ClinPharmacolToxicol. 2005 Oct;97(4):238-43. doi: 10.1111/j.1742-7843.2005.pto_136.x. PMID: 16176560.
Bellakhdar J, Claisse R , Fleurentin J, Younos C. Repertory of standard herbal drugs in the Moroccan pharmacopoeia. J Ethnopharmacol. 1991 ; 35: 123–143.
Tahraoui, A , El-Hilaly J, Israili ZH, Lyoussi B. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south- eastern Morocco (Errachidia province). J. Ethnopharmacol. 2007; 110: 105–117.
Bouyahya A, Chamkhi I, Benali T, Guaouguaou FE, Balahbib A, El Omari N, Taha D, Belmehdi O, Ghokhan Z, El Menyiy N. Traditional use, phytochemistry, toxicology, and pharmacology of Origanum majorana L. J Ethnopharmacol. 2021; 30;265:113318.
Busatta C, Vidal RS, Popiolski AS, Mossi AJ, Dariva C, Rodrigues MR, Corazza FC, Corazza ML, Vladimir Oliveira J, Cansian RL. Application of Origanum majorana L. essential oil as an antimicrobial agent in sausage. Food Microbiol. 2008; 25(1):207-11.
Hajlaoui H, Mighri H, Aouni M, Gharsallah N, Kadri A. Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti-acetylcholinesterase properties of Tunisian Origanum majorana L. essential oil. MicrobPathog. 2016; 95:86-94.
Hussain A I, Anwar F, Rasheed S, Nigam PS, Janneh O, Sarker SD. Composition, antioxidant and chemotherapeutic properties of the essential oils from two Origanum species growing in Pakistan.Rev. bras. farmacogn. 2011; 21, 943–952.
EuropeanPharmacopoeia (1975) Volume 3. Maisonneuve, Sainte-Ruffine.
Jilali SBE, Rachid I, Ghada B, Tarik M, Sanae R, Abderrazzak K. Effect of Isolation Techniques on the Quantity, Quality, and Antimicrobial Activity of Lavanduladentata Essential Oils. Trop J Nat Prod Res. 2023; 7(4):2713-2717
Adams R P. 2007. Identification of essential oil components by gas chromatography/ mass spectrometry, 4th Edition. Allured Publ., Carol Stream, IL
Odion EE, Falodun A, Adelusi SA. Total flavonoid, Total Phenolic and antioxidant potential of root bark extract and fractions of from Cola rostrata (Sterculiaceae) K. Schum. University of Benin . J Sci Tech .2013; 1 (2): 38 - 42.
Okolie NP, Falodun A, Davids O. Evaluation of the antioxidant activity of root extract of pepper fruit (Dennetiatripetala), and it's potential for the inhibition of lipid peroxidation. Afr J Tradit Complement Altern Med. 2014;11(3):221-7
Egharevba E, Chukwuemeke-Nwani P, Eboh U, Okoye E, Bolanle IO, Oseghale IO, Imieje VO, Erharuyi O, FalodunA.Antioxidant and Hypoglycaemic Potentials of the Leaf Extracts of Stachytarphytajamaicensis (Verbenaceae). Trop J Nat Prod Res. 2019; 3(5):170-174.
Oyaizu, M. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutrit Diet. 1986; 44: 307–315.
Furtado GL, Medeiros AA. Single-disk diffusion testing (Kirby-Bauer) of susceptibility of Proteus mirabilis to chloramphenicol: significance of the intermediate category. J ClinMicrobiol. 1980 ;12(4):550-3
National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A4. Wayne, Pa: National Committee for Clinical Laboratory Standards; 1999.
Remmal A, Bouchikhi T, Rhayour K. Improved method for the determination of antimicrobial activity of essential oils in agar medium. J Essent Oil Res.1993; 5:179–184.
Amor G, Caputo L, La Storia A, De Feo V, Mauriello G, Fechtali T. Chemical Composition and Antimicrobial Activity of Artemisia herba-alba and Origanum majorana Essential Oils from Morocco. Molecules. 2019; 6;24(22):4021.
Gales RC, Preotu A, Necula A, Gille E, Toma C. Altitudinal variations of morphology, distribution and secretion of glandular hairs in Orıganum vulgare L. leaves. StudiaUniv VG SSV. 2010; 20:59-62.
Hammam M, El-Sayed S, El-Sayed A, Mohamed W. Biochemical Studies On Antioxidant and Antimicrobial Activity Of Some Medicinal Plants Essential Oils. Menoufia J Agric Biotechnology. 2023; 2(8): 1-20.
Fliou J, Riffi O, Amechrouq A, Elhourri M, Ghouati Y. Comparative Study Of the Chemical Composition Of The Essential Oil Of Origanum Compactum From The Seven Regions Of Morocco And Their Antimicrobial Activity . J microb biotech food sci. 2020; 1(10): 42-48.
Yasar S, Nizamlıoğlu NM, Gücüş MO, Bildik Dal AE, Akgül K. Origanum majorana L. Essential Oil-Coated Paper Acts as an Antimicrobial and Antioxidant Agent against Meat Spoilage. ACS Omega. 2022 ;7(10):9033-9043.
Boureghida S, Slougui N, Fadel N, Hazmoune H, Bicha S. Chemical Composition and Antibacterial Activity of the Essential Oil from Erinaceaanthyllis Link (Fabaceae). Trop J Nat Prod Res. 2023; 7(4): 2696–2700.
Amorati R, Foti MC, Valgimigli L. Antioxidant activity of essential oils. J Agric Food Chem. 2013 ; 20, 61(46):10835-47.
Al-Saadi HK, Awad HA, Saltan ZS, Hasoon B A, Abdulwahab A I, Al-azawi K F, Al-Rubaii B A. Antioxidant and Antibacterial Activities of Allium sativum Ethanol Extract and Silver Nanoparticles Trop J Nat Prod Res. 2023; 7(6): 3105-3110.
El Atki,Y., Aouam, I., El Kamari, F., Taroq, A., Lyoussi, B., Oumokhtar, B., Abdellaoui,A. Phytochemistry, antioxidant and antibacterial activities of two Moroccan Teucriumpolium L. subspecies: Preventive approach against nosocomial infections, Arab J Chem. 2020;13 (2):3866-3874
Yanishleiva NV, Marinova EM, Gordon MH, Reneva VG, Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chem.1999; 64: 59–66.
Ruberto G, Baratta MT. Antioxidant activity of selected essential oil components in lipid model systems. Food Chem. 2000; 69: 167-174.
Wang W, Wu N, Zu YG, Fu YJ. Antioxidative activity of Rosmarinus officinalis L.615 essential oil compared to its main components. Food Chem. 2008; 108: 1019-1022.
Erdogan A, Ozkan A. Investigatıon of antioxıdative, cytotoxic, membrane damaging and membrane-protective effects of the essentıal oil of Origanum majorana and its oxygenated monoterpene component linalool in human-derived Hep G2 cell line. Iran J Pharm Res. 2017; 16(24): 24-34
Khadhri A, Bouali I, Aouadhi C, Lagel MC, Masson E, Pizzi A, Determination of phenolic compounds by MALDI–TOF and essential oil composition by GC–MS during three development stages of Origanum majorana L. Biomed Chromatogr. 2019; 33: e4665.
Makrane H, El Messaoudi M, Melhaoui A, El Mzibri M, Benbacer L, Aziz M. Cytotoxicity of the aqueous extract and organic fractions from Origanum majorana on human breast cell line MDA-MB-231 and human colon cell line HT-29. AdvPharmacol Sci. 2018; 23 :3297193.
Taamalli A, Arr´aez-Rom´an D, Abaza L, Iswaldi I, Fern´andez-Guti´errez A, Zarrouk M, Segura-Carretero A. LC-MS-based metabolite profiling of methanolic extracts from the medicinal and aromatic species Menthapulegium and Origanum majorana : phenolic compounds in Menthapulegium and Origanum majorana. Phytochem Anal. 2015; 26:320–330.
Andrews RE, Parks LW, Spence KD. Some effects of Douglas fir terpenesoncertain microorganisms. App EnvMicrobiol. 1980 ; 40: 301-304.
Cimanga K, Kambu K, Tona L, Apers S, De Bruyne T, Hermans N, Totte J Pieters L, Vlietinck AJ. Correlation between chemical composition and antibacterialactivity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. J Ethnopharmacol 2002; 79: 213-220.635
Chaves RDSB, Martins RL, Rodrigues ABL, Rabelo ÉM, Farias ALF, Brandão LB, Santos LL, Galardo AKR, de Almeida SSMDS. Evaluation of larvicidal potential against larvae of Aedesaegypti (Linnaeus, 1762) and of the antimicrobial activity of essential oil obtained from the leaves of Origanum majorana L. PLoS One. 2020;15(7):e0235740.
Omara ST, El-Moez SIA, Mohamed AM. Antibacterial effect of Origanum majorana L.(Marjoram) and Rosmarinus officinalis L.(Rosemary) essential oils on food borne pathogens isolated from raw minced meat in Egypt. Global Vet. 2014; 13: 1056–1064.
El Kamari F, Taroq A, Atki Y E, Aouam I, Lyoussi B, Abdellaoui A. Chemical composition of essential oils from Vitex Agnus-castus L. growing in morocco and its in vitro antibacterial activity against clinical bacteria responsible for nosocomial infections. Asian J Pharm Clin Res. 2018;11: 365-368.
El Atki Y, Aouam I, El Kamari F, Taroq A, Nayme K, Timinouni M, Lyoussi B, Abdellaoui A. Antibacterial activity of cinnamon essential oils and their synergistic potential with antibiotics. J Adv Pharm Technol Res. 2019; 10(2):63-67.
Djenane D, Yanguela J, Montanes L, Djerbal M, Roncales P. Antimicrobial activity of Pistacialentiscus and Saturejamontana essential oils against Listeria monocytogenes CECT 935 using laboratory media: efficacy and synergistic potential in minced beef. Food Control. 2011; 2(7): 1046-1053.660
Gupta SM, Arif M, Ahmed Z. Antimicrobial activity in leaf, seed extract and seed oil of Jatropha curcas L. Plant J App Nat Sci. 2011; 3: 102-105