Antihypertensive Effect of Moringa oleifera (Moringaceae) Methanolic Leaf Extract (MoMLE) on Cricetomys gambianus (Muridae)

Elijah C. Odiia,f, Joseph O. Okoroa,f*, Augustine N. Okorieb, Chinweike N. Asogwaa, Christian O. Chukwukag, Charles O. Okoyea,e,f, Emmanuel S. Okeke c,d,e,f*, Vincent C. Ejerea, Joseph E. Eyoa

aDepartment of Zoology and Environmental Biology, University of Nigeria, Nsukka, 410001, Enugu State, Nigeria.
bDepartment of Pharmacology, University of Nigeria, Nsukka, Enugu State, Nigeria.
cDepartment of Biochemistry, University of Nigeria, Nsukka, 41000, Enugu State, Nigeria
dNatural Science Unit, SGS, University of Nigeria Nsukka, 410001 Enugu State, Nigeria
eSchool of Environment and Safety Engineering, Jiangsu University, 212013 P.R. China
fOrganization of African Academic Doctors (OAAD), Off Kamiti Road, P. O. Box 25305000100, Nairobi, Kenya
gDepartment of Zoology, University of Otago, Dunedin, New Zealand

Corresponding Author: [email protected]; [email protected]; Tel: +2347033065065; +2348035277554
Recieved Date: 18 March 2022; Accepted Date: 11 October 2022; Published Date: 01 November
Odii EC, Okoro JO, Okorie AN, Asogwa CN, Chukwuka CO, Okoye CO, Okeke ES, Ejere VC, Eyo JE. Antihypertensive Effect of Moringa oleifera (Moringaceae) Methanolic Leaf Extract (MoMLE) on Cricetomys gambianus (Muridae). Trop J Nat Prod Res. 2022; 6(10):1695-1700.
© 2022 Odii et al. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Cardiovascular diseases are on the rise around the world and have become some of the leading causes of death. To enhance their chances of survival and effectively manage costs, indigenous communities in developing countries have sought natural remedies from plants and food materials within their reach. This established preference has seen Moringa oleifera garner special attention due to the praised effects of its leaves and other parts. The focus of this study was to investigate the antihypertensive activity of crude MoMLE (CME) and five solvent fractions of the same using C. gambianus and elucidate the effect of their separate interactions with atropine. Moringa oleifera (MO) leaf was collected from the University of Nigeria, Nsukka; C. gambianus was obtained from Okposi-Okwu, Ebonyi State; and antihypertensive activity was assessed using a blood pressure transducer following established methods. The result showed that CME and other fractions had a dose-dependent negative inotropic and chronotropic effect on C. gambianus as follows: N-Hexane fraction (NHF)-19.0 8.1 mmHg, chloroform fraction (CLF)-15.0 3.0 mmHg, ethyl acetate fraction (EAF)-22.3 10.7 mmHg, acetone fraction (ACF)-19.7 7.2 mmHg, and methanol fraction (MEF)-25.7 1.2 mmHg. Similar activity was observed for acetylcholine (-42 ± 4.4 mmHg) which was used as a reference drug (positive control). The study revealed that MoMLE has a hypotensive effect with methanol fraction (MEF) having the second highest hypotensive (-25.7 ± 1.2 mmHg) activity after CME with a 28.7 ± 4.2 mmHg lowering effect. Atropine interaction blocked the extracts as well as acetylcholine, suggesting a muscarinic-receptor mediated action.

Keywords: Acetylcholine, Antihypertensive, C. gambianus, Hypotensive effect, Moringa oleifera 
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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