Combination of Hydration and Ascorbic Acid Synergistically Prevents Organophosphate Pesticide Toxicity of Vital Organs in Experimental Rat Model

Ikenna K. Uchendu1*, Ozioma E. Obianyido1, Kelvin N. Orji1, Amarachi C. Uba2, Juliet U. Joboson1, Cyprian E. Oguji1, Ebubechukwu L. Ofojama1, Ukam E. Etim1, Blessing C. Oguzie3, Ifunanyachukwu C. Onuchukwu3, Chibuike E. Maduekwe1, Chikwube C. Ajibo1, Favour C. Sunday1, Comfort C. Igwe1, Chimdike G. Omejua1, Chibueze J. Obigeorge, Chisom N. Okafor1, Victor C. Makata1, Sharon O. Eze1, Onyinyechi V. Ifionu1, Chisimdindu F. Nnaji1, Vivian O. Nnanyereugo1, Carmilla I. Udensi1, Hilary Emuebie1, Ejiofor M. Agbo1, Olayinka T. Amokeoja4, Chiagoziem H. Ekemezie5, Unoaku J. Ikwueze1 Sheriffdeen A. Adebisi6, Prince I. Onuoha7

1Department of Medical Laboratory Science, University of Nigeria Enugu Campus, Enugu State, Nigeria
2Department of Biomedical Technology, University of Port Hacourt, Rivers State, Nigeria
3Department of Medical Laboratory Science, Abia State University, Uturu, Abia State, Nigeria
4Department of Health Information Management, Obafemi Awolowo University Teaching Hospital, Ife, Osun State, Nigeria
5Department of Medical Laboratory Science, Nnamdi Azikikwe University, Awka, Anambra State, Nigeria
6Department of Pharmacy, Olabisi Onabanjo University. Ogun State, Nigeria
7Department of Medical Laboratory Science, University of Lagos, Lagos State, Nigeria

Corresponding Author: [email protected]; Tel: +2347068199556
Recieved Date: 15 June 2022; Accepted Date: 18 August 2022; Published Date: 03 September
Citation:  Uchendu IK, Obianyido OE, Orji KN, Uba AC, Joboson JU, Oguji CE, Ofojama EL, Etim UE, Oguzie BC, Onuchukwu IC, Maduekwe CE, Ajibo CC, Sunday FC, Igwe CC, Omejua CG, Obigeorge CJ, Okafor CN. Makata VC, Eze SO, Ifionu OV, Nnaji CF, Nnanyereugo VO, Udensi CI, Emuebie H, Agbo EM, Amokeoja OT, Ekemezie CH, Ikwueze UJ, Adebisi SA, Onuoha PI. Combination of Hydration and Ascorbic Acid Synergistically Prevents Organophosphate Pesticide Toxicity of Vital Organs in Experimental Rat Model. Trop J Nat Prod Res. 2022; 6(8):1297-1304.
© 2022 Uchendu et al. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Triazophos, an organophosphate acetylcholinesterase inhibitor and synthetic organic thiophosphate molecule, is employed as a pesticide. It is a yellowish-brown oily liquid, and exposure can happen through inhalation, ingestion, or touch. This study evaluated the toxic effects of organophosphate pesticide exposure on several vital organs, and the therapeutic potential of ascorbic acid (vitamin C) or water hydration alone or their combination. Random grouping of 25 adult male Wistar rats into five (A-E) was done. A-D Groups received pesticide (0.5mL/kg, i.p) for 3 days. B and D groups were treated with Vitamin C (200mg/kg, oral) only. Groups C and D received oral hydration with distilled water only (15mL/kg) for 3 days. Only pesticide was given to group A (negative control); while group E received no administration. The functions of the kidneys, liver, testicles and heart respectively were determined by measuring serum levels of urea, creatinine, K+and Na+; Alanine transaminase (ALT), Aspatate transaminase (AST), Alkaline phosphatase (ALP) And total bilirubin; testosterone; and lipid profiles (Total cholesterol (TC), Triglycerides (TG), and Low density lipoprotein cholesterol (LDL-C) and by histopathological examination of the kidneys, liver, testes and heart using standard methods. The pesticide exposure alone caused a marked toxicity to the vital organs. Vitamin C alone showed significant ameliorative effect on the organs (p<0.05). Distilled water alone indicated a non-significant difference improvement (p>0.05).  The findings of histopathology matched the biochemical findings. The combination of water hydration and ascorbic acid synergistically protected these vital organs against toxic chemicals.

Keywords: Organ toxicity, Organophosphate pesticides, Vitamin C, Water hydration 
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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