Antioxidant and Alpha Amylase Inhibitory Potential of Methanol Extract of Unripe Musa paradisiaca Fruit with Husk

*Maryam U. Ahmed1, Ushuwa Bala1, Isaac J. Umaru2
1Department of Biochemistry, Adamawa State University, Mubi, Adamawa, Nigeria
2Department of Biochemistry, Federal University, Wukari, Taraba State, Nigeria

Corresponding Author: [email protected]; Tel: +2348038279992
Recieved Date: 31 May 2022; Accepted Date: 26 August 2022; Published Date: 03 September
Citation: Ahmed MU, Bala U, Umaru IJ. Antioxidant and Alpha Amylase Inhibitory Potential of Methanol Extract of Unripe Musa paradisiaca Fruit with Dried Husk. Trop J Nat Prod Res. 2022; 6(8):1281-1285 http://www.doi.org/10.26538/tjnpr/v6i8.20
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© 2022 Ahmed et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Diabetes mellitus is a leading cause of death in adults. The study aimed at evaluating the antioxidant and alpha amylase inhibitory potential of methanol extract of unripe Musa paradisiaca   Linn fruit with husk. Ferric reducing antioxidant power (FRAP) scavenging activity, diphenyl -1- picrlhydrazyl (DPPH) free radical scavenging activity, alpha amylase inhibitory potential and the mode of inhibition of methanol extract of unripe Musa paradisiaca Linn fruit with husk were determined using standard methods. The DPPH IC50 of the methanol extract of unripe Musa paradisiaca Linn fruit with husk was comparable with the standard; ascorbic acid. The percentage inhibition of methanol extract of unripe Musa paradisiaca Linn fruit with husk at 2.50 mg/mL and 10.0 mg/mL were greater than 50% (67% and 73%). Acarbose at 1.25 mg/mL, 2.50 mg/mL and 10 mg/mL had a percentage inhibition of 61%, 67% and 70% respectively. The maximum velocity (Vmax) of the enzyme in the presence of the unripe Musa paradisiaca with dried husk extract (inhibitor) was 0.8 mM/min and it was also 0.8 mM/min in the absence of inhibitor (no plant extract). The Michealis-Menten constant (Km) of the enzyme in the presence of the inhibitor was 1.3 mg/mL, while it was 0.7 mg/mL in the absence of the inhibitor.  This study concluded that the methanol extract of unripe Musa paradisiaca Linn fruit with husk has high antioxidant capacity. It also inhibits alpha amylase in a competitive way. It can therefore be used to treat diabetes mellitus 

Keywords: Alpha amylase, Diabetes, Antioxidant, Oxidative stress, Unripe M. paradisiaca 
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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