Investigating the Effect of Flavonoid, Saponin, Alkaloids and Tannins Extracted from Combretum dolichopentalum Diels in CCl4 –Induced Hepatotoxicity

Favour N.Ujowundu*, James O. Kalu, Cosmas O. Ujowundu, Ignatius O. Onyeocha, Chinyere H. Onuoha, Raymond C. Ibeh, Uchechi K. Obasi, Oluwatosin E. Ntaji, Ifeyinwa N. Chigbu, Chioma Y. Ezirim
Federal University of Technology Owerri, Imo State Nigeria

Corresponding Author: [email protected]; Tel: +2348034741650
Recieved Date: 03 April 2022; Accepted Date: 19 August 2022; Published Date: 03 September
Citation: Ujowundu FN, Kalu JO, Ujowundu CO, Onyeocha IO, Onuoha CH, Ibeh RC, Obasi UK, Ntaji OE, Chigbu IF, Ezirim CY.Investigating the Effect of Flavonoid, Saponin, Alkaloids and Tannins Extracted from Combretum dolichopentalum Diels in CCl4 –Induced Hepatotoxicity.Trop J Nat Prod Res. 2022; 6(8):1255-1261. http://www.doi.org/10.26538/tjnpr/v6i8.16
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© 2022 Ujowunduet al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Modification of biological molecules caused by oxidative stress is the most popular harmful biological side reaction leading to pathological changes. This study investigated the impact of flavonoids, alkaloids, saponins, and tannin extracted from Combretum dolichopentalum on CCl4-induced hepatotoxicity. Sixty-five male rats were sorted into nine groups, and allowed access to water and food. Groups I and II received distilled water only, groups III and IV pre-treated with 250 and 500 mg/kg body weight (bwt) ethanol extract of C. dolichopentalum (EECD) respectively, group V pre-treated with 50 mg/kg bwt silymarin, group VI to IX pre-treated with 100 mg/kg bwt of flavonoids, saponins, alkaloids and tannin of C. dolichopentalum respectively. After seven days, all animals (except normal control) were intoxicated with 0.4 mL/kg bwt CCl4, and sacrificed 48 h after. Samples of liver and blood were used for histology and biochemical studies respectively. Results showed that CCl4 -induced massive fatty change and centrilobular necrosis in the liver, marked elevations in serum malondialdehyde (MDA), liver marker enzymes and other biochemical changes were observed. However, animals administered EECD, alkaloids, flavonoids, tannins and saponins of C. dolichopentalum presented improved liver histological feature. The biochemical parameters of treated rats approached that of normal control while rats administered alkaloid and flavonoid showed more significant decrease in MDA and total bilirubin. The results from the current study show that C. dolichopentalum, especially flavonoid and alkaloid extracts, clearly protects the liver from CCl4-induced hepatotoxicity. Furthermore, the saponin fraction of C. dolichopentalum appears to be hepatotoxic to the liver.

Keywords: Combretum dolichopentalum, Flavonoids, Saponins, Tannins, Hepatotoxicity. 
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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